Neuronal and glial cell co-culture organization and impedance spectroscopy on nanocolumnar TiN films for lab-on-a-chip devices

Abend, Alice; Steele, Chelsie; Schmidt, Sabine; Frank, Ronny; Jahnke, Heinz‐Georg; Zink, Mareike

doi:10.1039/d2bm01066f

Cited by 3 publications

(1 citation statement)

References 61 publications

(100 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Combined with the use of scanning electron micrographs (SEM) to characterize the electrode morphology, it can more effectively display the impact of the material surface on cells. [183][184][185] On the other hand, the direct in vivo implantation method is to implant the material into the appropriate part of the animal. After one cycle of observation, the distribution of glial cells and neurons around the wound is displayed according to histological analysis and immunofluorescence staining, which is used to determine the histomorphology and cellular changes around the electrode, including detection of neuronal nuclei (NeuN), glial fibrillary acid protein (GFAP), microglial and macrophage markers (Iba1 and CD68/ED1), immunoglobulin gamma (IgG), nuclear chromatin markers (DAPI: 4 0 ,6-diamidino-2-phenylindole) and other indicators.…”

Section: Neural Electrodes Integrated With Microfluidic Channelsmentioning

confidence: 99%

Implantable neural electrodes: from preparation optimization to application

et al. 2023

View full text Add to dashboard Cite

show abstract

Section: Neural Electrodes Integrated With Microfluidic Channelsmentioning

confidence: 99%

Implantable neural electrodes: from preparation optimization to application

et al. 2023

View full text Add to dashboard Cite

show abstract

Challenges and Opportunities of Implantable Neural Interfaces: From Material, Electrochemical and Biological Perspectives

Zeng

Huang

2023

Adv Funct Materials

View full text Add to dashboard Cite

The desirable implantable neural interfaces can accurately record bioelectrical signals from neurons and regulate neural activities with high spatial/time resolution, facilitating the understanding of neuronal functions and dynamics. However, the electrochemical performance (impedance, charge storage/injection capacity) is limited with the miniaturization and integration of neural electrodes. The “crosstalk” caused by the uneven distribution of elctric field leads to lower electrical stimulation/recording efficiency. The mismatch between stiff electrodes and soft tissues exacerbates the inflammatory responses, thus weakening the transmission of signals. Though remarkable breakthroughs have been made through the incorporation of optimizing electrode design and functionalized nanomaterials, the chronic stability, and long‐term activity in vivo of the neural electrodes still need further development. In this review, the neural interface challenges mainly on electrochemistry and biology are discussed, followed by summarizing typical electrode optimization technologies and exploring recent advances in the application of nanomaterials, based on traditional metallic materials, emerging 2D materials, conducting polymer hydrogels, etc., for enhancing neural interfaces. The strategies for improving the durability including enhanced adhesion and minimized inflammatory response, are also summarized. The promising directions are finally presented to provide enlightenment for high‐performance neural interfaces in future, which will promote profound progress in neuroscience research.

show abstract

Organisation von Gehirnzellen auf nanostrukturierten Materialien

Abend,

Jahnke,

Zink

2024

Biospektrum

View full text Add to dashboard Cite

Brain-on-a-chip devices offer great potential to study function and disease of the brain. We employ machine-learning algorithms in combination with fluorescence imaging and adhesion studies of neuronal cells to access the biocompatibility of electrode materials. Multielectrode arrays of nanocolumnar titanium nitride comprise improved electric properties and cell-surface interaction compared to conventional electrode materials important for cell stimulation.

show abstract

Neuronal and glial cell co-culture organization and impedance spectroscopy on nanocolumnar TiN films for lab-on-a-chip devices

Abstract: Co-cultured neuronal SH-SY5Y and U-87 MG cells grown with various ratios on TiN, TiN nano, and ITO exhibit distinct cellular organization, proliferation, and electrochemical impedance results depending on cell ratio and electrode material.

Cited by 3 publications

References 61 publications

Implantable neural electrodes: from preparation optimization to application

Implantable neural electrodes: from preparation optimization to application

Challenges and Opportunities of Implantable Neural Interfaces: From Material, Electrochemical and Biological Perspectives

Organisation von Gehirnzellen auf nanostrukturierten Materialien

Contact Info

Product

Resources

About