Neuronal correlates of 5-hydroxytryptophan-induced sustained swimming in Aplysia fasciata

Kabotyanski, E. A.; Milošević, Ivan; Sakharov, D. A.

doi:10.1016/0742-8413(90)90080-s

Cited by 8 publications

(8 citation statements)

References 13 publications

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“…These results were obtained (and have been published in part as conference proceedings [26]) by building on our previously accumulated experience of mediator manipulation of the mollusk locomotor generators [2,7,15,21,22].…”

supporting

confidence: 55%

“…Synchronization of paired pedal locomotion generators has previously been demonstrated in the sea angel (Clione) [4]. The paired 5-HT-dependent generators controlling locomotion in Aplysia are located similarly [15]. Despite the external differences in the locomotor organs, all are derivatives of the same foot and body wall muscles.…”

Section: Discussionmentioning

confidence: 95%

“…High doses of 5-HT induced hyperlocomotion in these mollusks, i.e., the most intense method of locomotion (rapid hunting-type swimming in Clione limacina which initially swam in a "cruising" manner; prolonged--for several hours--continuous swimming in initially gliding Aplysia fasciata [2,7,15]). Freshwater snails--the pond snail and the flateye snail--are exceptions in this regard: after injection of 5-HTP, we observed then rather poorly understood rhythmic movements of the shell [16].…”

Section: Discussionmentioning

confidence: 99%

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Transmitter-dependent switching of respiratory interneurons to the locomotor rhythm in the pulmonate molluskLymnaea

Sakharov

Tsyganov

2000

Neurosci Behav Physiol

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Cell RPeD1 of the pond snail Lymnaea stagnalis is a dopaminergic neuron known to be involved in generating the respiratory rhythm. This report describes conditions in which RPeD1 follows the rhythm of another central generator, identified as the generator of muscular locomotion. This rhythmic activity of RPeD1 developed in isolated CNS preparations treated with 0.05 mM serotonin (5-hydroxytryptamine, 5-HT) or 0.1 mM 5-hydroxytryptophan (5-HTP). It was coordinated by cyclic synaptic currents with the activities of other pedal neurons and with trains of spikes in the motor (pedal and columellar) nerves. Only the pedal ganglia (both of them) retained the ability to generate 5-HT-dependent cyclic activity after complete isolation, indicating a pedal localization and paired nature for the central generator. In intact animals, injection of 100 microg/g of 5-HTP induced coordinated cyclic movements of the foot and shell which had similar periods and resembled movements during terrestrial (non-ciliary) locomotion. These results, along with the known role of dopamine in generating the respiratory pattern, show that switching of RPeD1 from one central rhythm to another is a transmitter-dependent phenomenon.

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supporting

confidence: 55%

Section: Discussionmentioning

confidence: 95%

Section: Discussionmentioning

confidence: 99%

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Transmitter-dependent switching of respiratory interneurons to the locomotor rhythm in the pulmonate molluskLymnaea

Sakharov

Tsyganov

2000

Neurosci Behav Physiol

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“…Exogenously applied 5-HT and 5-HTP modulate GPG-generated rhythmic activity, including locomotion, swimming, respiration, and chewing, in many invertebrates and vertebrates (Willard, 1981;Hashemzadeh-Gargari and Friesen, 1989;Kabotyanski et al, 1990;Kabotyanskii and Sakharov, 1991;Satterlie and Norekian, 1995;Norekian and Satterlie, 1996). The effects of 5-HT on the leech swim-initiating interneuron, cell 204, is of special interest because 5-HT increases the ability of cell 204 to elicit swimming in a manner similar to the effect of 5-HTP treatment on DSI potency.…”

Section: -Ht Augmentation and Central Pattern Generationmentioning

confidence: 99%

Paradoxical Actions of the Serotonin Precursor 5-hydroxytryptophan on the Activity of Identified Serotonergic Neurons in a Simple Motor Circuit

Fickbohm

Katz

2000

J. Neurosci.

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Neurotransmitter synthesis is regulated by a variety of factors, yet the effect of altering transmitter content on the operation of neuronal circuits has been relatively unexplored. We used electrophysiological, electrochemical, and immunohistochemical techniques to investigate the effects of augmenting the serotonin (5-HT) content of identified serotonergic neurons embedded in a simple motor circuit. The dorsal swim interneurons (DSIs) are serotonergic neurons intrinsic to the central pattern generator (CPG) for swimming in the mollusc Tritonia diomedea. As expected, treatment with the serotonin precursor 5-hydroxytryptophan (5-HTP) increased the intensity of serotonin immunolabeling and enhanced the potency of synaptic and modulatory actions elicited by the DSIs. It also greatly enhanced the ability of the DSIs to evoke rhythmic CPG activity. After 5-HTP treatment, microvoltammetric measurements indicated an increase in a putative 5-HT electrochemical signal during swim CPG activation. Paradoxically, the spiking activity of the serotonergic neurons decreased to a single burst at the onset of the rhythmic motor program, whereas the overall duration of the episode remained about the same. 5-HTP treatment gradually reduced the rhythmicity of the CPG output. Thus, more serotonin did not result in a more robust swim motor program, suggesting that serotonin synthesis must be kept within certain limits for the circuit to function correctly and indicating that altering neurotransmitter synthesis can have serious consequences for the output of neural networks.

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“…These cells are known to deliver serotonin to various locomotor organs of Lymnaea stagnalis, specifically the ciliated epithelium of the sole and locomotor muscles (Syed and Winlow, 1989). Studies on neurons homologous to PeA neurons in the swimming marine gastropods Clione limacina and Aplysia fasciata have shown that strong, longlasting excitation of these pedal serotonergic neurons is correlated with locomotor arousal (Kabotyanski et al, 1990;Kabotyanski and Sakharov, 1991;Satterlie, 1995). Locomotor arousal is associated with moderate hunger and food searching behaviour in many animals, including Lymnaea (Dyakonova and Sakharov, 1995).…”

Section: Storage Of the Behavioural State In Completely Isolated Neuronsmentioning

confidence: 99%

The Activity of Isolated Neurons and the Modulatory State of an Isolated Nervous System Represent a Recent Behavioural State

Dyakonova

Hernádi

Ito

et al. 2015

Journal of Experimental Biology

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Behavioural/motivational state is known to influence nearly all aspects of physiology and behaviour. The cellular basis of behavioural state control is only partially understood. Our investigation, performed on the pond snail Lymnaea stagnalis whose nervous system is useful for work on completely isolated neurons, provided several results related to this problem. First, we demonstrated that the behavioural state can produce long-term changes in individual neurons that persist even after neuron isolation from the nervous system. Specifically, we found that pedal serotonergic neurons that control locomotion show higher activity and lower membrane potential after being isolated from the nervous systems of hungry animals. Second, we showed that the modulatory state (the chemical neuroactive microenvironment of the central ganglia) changes in accordance with the nutritional state of an animal and produces predicted changes in single isolated locomotor neurons. Third, we report that observed hunger-induced effects can be explained by the increased synthesis of serotonin in pedal serotonergic neurons, which has an impact on the electrical activity of isolated serotonergic neurons and the intensity of extrasynaptic serotonin release from the pedal ganglia.

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Neuronal correlates of 5-hydroxytryptophan-induced sustained swimming in Aplysia fasciata

Cited by 8 publications

References 13 publications

Transmitter-dependent switching of respiratory interneurons to the locomotor rhythm in the pulmonate molluskLymnaea

Transmitter-dependent switching of respiratory interneurons to the locomotor rhythm in the pulmonate molluskLymnaea

Paradoxical Actions of the Serotonin Precursor 5-hydroxytryptophan on the Activity of Identified Serotonergic Neurons in a Simple Motor Circuit

The Activity of Isolated Neurons and the Modulatory State of an Isolated Nervous System Represent a Recent Behavioural State

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