Neuronal damage is much delayed and microgliosis is more severe in the aged hippocampus induced by transient cerebral ischemia compared to the adult hippocampus

Lee, Choong Hyun; Yoo, Ki‐Yeon; Choi, Jung Hoon; Park, Ok Kyu; Hwang, In Koo; Kim, Sung Koo; Kang, Il‐Jun; Kim, Young‐Myeong; Won, Moo‐Ho

doi:10.1016/j.jns.2010.04.014

Cited by 47 publications

(46 citation statements)

References 29 publications

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“…It has also been reported that transition of microglia from normal resting state to activated state are observed in various neurological diseases conditions [26,49]. It was reported that Iba-1 immunoreactivity in the hippocampus of the aged-control-group was increased compared to that in the adult-control-group [7,49]. This previous study is consistent with our present result.…”

Section: Discussionsupporting

confidence: 93%

“…In addition, recent reports have reported that GR expresses in microglia in experiments in vitro and in vivo [23,48]. It has also been reported that transition of microglia from normal resting state to activated state are observed in various neurological diseases conditions [26,49]. It was reported that Iba-1 immunoreactivity in the hippocampus of the aged-control-group was increased compared to that in the adult-control-group [7,49].…”

Section: Discussionmentioning

confidence: 95%

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Comparison of Glucocorticoid Receptor and Ionized Calcium-Binding Adapter Molecule 1 Immunoreactivity in the Adult and Aged Gerbil Hippocampus Following Repeated Restraint Stress

et al. 2011

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Stress leads to changes in homeostasis and internal balance of the body and is known to be one of important factors in the development of several diseases. In the present study, we investigated changes in glucocorticoid receptor (GR) and ionized calcium-binding adapter molecule 1 (Iba-1) immunoreactivity in the adult and aged gerbil hippocampus after chronic restraint stress. Serum corticosterone level was much higher in both the stress-groups than the control groups. No neuronal death was found in all hippocampal subregions of the adult and aged gerbil after chronic restraint stress. GR immunoreactivity was decreased in both the adult and aged groups after repeated restraint stress; however, GR immunoreactivity in the adult-stress-group was decreased much more than that in the aged-stress-group. Iba-1 immunoreactive microglia were hypertrophied and activated in the adult group after repeated restraint stress; in the aged-stress-group, there was no any significant change in Iba-1 immunoreactive microglia. In brief, level of GR, not Iba-1, in the hippocampus was much decreased in the adult gerbil compared to the aged gerbil following chronic restraint stress.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionmentioning

confidence: 95%

Comparison of Glucocorticoid Receptor and Ionized Calcium-Binding Adapter Molecule 1 Immunoreactivity in the Adult and Aged Gerbil Hippocampus Following Repeated Restraint Stress

et al. 2011

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“…Though the exact reason why the ratio of POCD in the old was further higher than in the young has not been expound, we concluded several main viewpoints as following: 1) pathophysiological changes in the central nervous system of the elderly themselves; 2) cerebral microthrombosis and vasospasm induced by atherosclerosis in the elderly population (Evered et al, 2010); 3) the elderly were more susceptible to the adverse impact of ischemic injury (Lee et al, 2010); and 4) the elderly had poor tolerance to stress response. The CPB has been used in the majority of the cardiac surgeries, which was also an interrupting factor for POCD.…”

Section: Discussionmentioning

confidence: 86%

The effects of electroacupuncture treatment on the postoperative cognitive function in aged rats with acute myocardial ischemia–reperfusion

Yuan

Zhang

et al. 2014

Brain Research

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“…To confirm the delayed neuronal death in the hippocampal CA1 region between the adult and aged gerbils following transient cerebral ischemia, NeuN immunohistochemistry was performed, according to the methods of the previous studies (27,28). In brief, for NeuN immunohistochemistry, the sections were sequentially treated with 0.3% hydrogen peroxide (H 2 O 2 ; Sigma-Aldrich) in PBS for 30 min and 10% normal goat serum (Vector Laboratories, Inc., Burlingame, CA, USA) in 0.05 M PBS for 30 min.…”

Section: Methodsmentioning

confidence: 99%

Differences in the protein expression levels of Trx2 and Prx3 in the hippocampal CA1 region between adult and aged gerbils following transient global cerebral ischemia

Lee

Park

Cho

et al. 2015

Molecular Medicine Reports

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The thioredoxin (Trx) and peroxiredoxin (Prx) redox system is associated with neuronal damage and neuroprotective effects via the regulation of oxidative stress in brain ischemia. In the present study, ischemia-induced changes in the protein expression levels of Trx2 and Prx3 in the stratum pyramidale (SP) of the hippocampal CA1 region were investigated in adult and aged gerbils, subjected to 5 min of transient global cerebral ischemia, using immunohistochemistry and western blot analysis. In the adult ischemia-group, minimal Trx2 immunoreactivity was detected in the SP 2 days after ischemia-reperfusion. In the aged animals, the Trx2 immunoreactivity in the sham-group was marginally lower compared with that in the adult sham-group. In the aged ischemia-group, Trx2 immunoreactivity in the SP was significantly higher 1, 2 and 4 days post-ischemia, compared with that in the adult ischemia-group and, in the 5 days post-ischemia group, Trx2 immunoreactivity was significantly decreased in the SP. Prx3 immunoreactivity in the SP of the adult ischemia-group was significantly decreased from 4 days after ischemia-reperfusion. In the aged animals, Prx3 immunoreactivity in the sham-group was also marginally lower compared with that in the adult sham-group. Prx3 immunoreactivity in the aged ischemia-group was also significantly higher 1, 2 and 4 days post-ischemia, compared with the adult ischemia-group; however, the Prx3 immunoreactivity was significantly decreased 5 days post-ischemia. The western blot analyses revealed that the pattern of changes in the protein levels of Trx2 and Prx3 in the adult and aged hippocampal CA1 region following ischemic damage were similar to the results obtained in the immunohistochemical data. These findings indicated that cerebral ischemia lead to different protein expression levels of Trx2 and Prx3 in the hippocampal CA1 region between adult and aged gerbils, and these differences may be associated with more delayed neuronal death in the aged gerbil hippocampus following transient global cerebral ischemia.

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Neuronal damage is much delayed and microgliosis is more severe in the aged hippocampus induced by transient cerebral ischemia compared to the adult hippocampus

Cited by 47 publications

References 29 publications

Comparison of Glucocorticoid Receptor and Ionized Calcium-Binding Adapter Molecule 1 Immunoreactivity in the Adult and Aged Gerbil Hippocampus Following Repeated Restraint Stress

Comparison of Glucocorticoid Receptor and Ionized Calcium-Binding Adapter Molecule 1 Immunoreactivity in the Adult and Aged Gerbil Hippocampus Following Repeated Restraint Stress

The effects of electroacupuncture treatment on the postoperative cognitive function in aged rats with acute myocardial ischemia–reperfusion

Differences in the protein expression levels of Trx2 and Prx3 in the hippocampal CA1 region between adult and aged gerbils following transient global cerebral ischemia

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