Neuronal differentiation of PC12 cells and mouse neural stem cells on carbon nanotube films

Посыпанова, Г. А.; Gayduchenko, Igor; Moskaleva, E. Yu.; Fedorov, G.

doi:10.1134/s1990519x16030111

Cited by 6 publications

(4 citation statements)

References 25 publications

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“…This also suggests that electrically conductive BC/PVAN/PANI/CNT nanocomposites are more favorable for neuronal differentiation than nonconductive BC. In fact, the microporous structure, the surface morphology, and the electrical properties, in particular those of grafted CNTs, can play a vital role in cell adhesion and neurite outgrowth. , On the basis of these findings, the electrically conductive BC/PVAN/PANI/CNT nanocomposites can potentially act as a biological interface for highly specialized biomedical devices, either as an electrical stimulator through a deliverable signal input or as a recorder …”

Section: Resultsmentioning

confidence: 99%

“…80,82 On the basis of these findings, the electrically conductive BC/PVAN/PANI/CNT nanocomposites can potentially act as a biological interface for highly specialized biomedical devices, either as an electrical stimulator through a deliverable signal input or as a recorder. 83 As schematically illustrated in Figure 14, the BC/PVAN/ PANI/CNT nanocomposite membranes, with a 3D porous nanofibrillar network, can support the cell adhesion and growth of neural stem cells with multiple focal points through the localized cell/substrate interactions. Nonsignaling cells (i.e., at resting potential) are negatively charged, with a higher concentration of positive ions in the surrounding areas than in the intracellular space.…”

Section: Thermal Stabilitymentioning

confidence: 99%

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Carbon Nanotube-Reinforced Poly(4-vinylaniline)/Polyaniline Bilayer-Grafted Bacterial Cellulose for Bioelectronic Applications

Rebelo

Liu

et al. 2019

ACS Biomater. Sci. Eng.

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Microbial cellulose paper treated with polyaniline and carbon nanotubes (PANI/CNTs) can be attractive as potential flexible capacitors in terms of further improvements to the conductivity and thermal resistance. The interactions between PANI and CNTs exhibit new electrochemical features with increased electrical conductivity and enhanced capacity. In this study, PANI/CNTs was incorporated into a flexible poly(4-vinylaniline)-grafted bacterial cellulose (BC/PVAN) nanocomposite substrate for further functionalization and processability. PANI/CNTs coatings with a nanorod-like structure can promote an efficient ion diffusion and charge transfer, with a significant enhancement of the electrical conductivity after CNTs reinforcement of 1 order of magnitude up to (1.0 ± 0.3) × 10–1 S·cm–1. An escalating improvement of the double charge capacity (∼54 mF) of the grafted BC nanocomposites was also detected through electrochemical analysis. The multilayered electrical coatings also reinforce the thermal resistance, preventing anticipated thermal degradation of the BC substrate. The cell viability and differentiation assays using neural stem cells (SVZ cells) testified to the cytocompatibility of the grafted BC nanocomposites, while inducing neuronal differentiation over 7 days of culture with a neurite that was 77 ± 24.7 μm long. This is promising for meeting the requirements in the construction of high-performance bioelectronic devices that can actively interface biologically, providing a friendly environment for cells while tuning the device performance.

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Section: Resultsmentioning

confidence: 99%

Section: Thermal Stabilitymentioning

confidence: 99%

Carbon Nanotube-Reinforced Poly(4-vinylaniline)/Polyaniline Bilayer-Grafted Bacterial Cellulose for Bioelectronic Applications

Rebelo

Liu

et al. 2019

ACS Biomater. Sci. Eng.

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“…These materials can be assembled in the form of wrapped graphite sheets, and therefore called carbon nanotubes (CNTs), or in the form of graphene foams. Both cases were proven to be biocompatible and conductive scaffolds not only for culturing primary neurons, but also for neural stem cell differentiation (49,50). Interestingly, primary hippocampal neurons plated on 3D graphene showed a more frequent and synchronized spontaneous activity comparing to both 2D graphene and traditional 2D glass substrates (51).…”

Section: D Neuronal Cultures -Methods Applications and Spontaneous Activitymentioning

confidence: 99%

Evaluation of in vitro neuronal networks for the study of spontaneous activity

Pozzi

2020

STEMedicine

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In the absence of external stimuli, the nervous system exhibits a spontaneous electrical activity whose functions are not fully understood, and that represents the background noise of brain operations. Spontaneous activity has been proven to arise not only in vivo, but in in vitro neuronal networks as well, following some stereotypical patterns that reproduce the time course of development of the mammalian nervous system. This review provides an overview of in vitro models for the study of spontaneous network activity, discussing their ability to reproduce in vivo - like dynamics and the main findings obtained with each particular model. While explanted brain slices are able to reproduce the neuronal oscillations typically observed in anaesthetized animals, dissociated cultures allow the use of patient-derived neurons and limit the number of animals used for sample preparation.

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“…The linear alignment of CNT structure gives a proper medium for the cells to contact with each other through synapse. It improves the adhesion and interaction of cells on CNT due to its affinity toward different proteins, such as laminin and fibronectin [124]. It also increases the expression of microtubule-associated protein (MAP2) [125].…”

Section: Effect On Differentiation Of Neural Cellsmentioning

confidence: 99%

Electrical Stimulation-Mediated Differentiation of Neural Cells on Conductive Carbon Nanofiller-Based Scaffold

Kaushik

Khatua

Ghosh

et al. 2022

Biomedical Materials & Devices

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An important strategy in neural tissue engineering involves imparting electrical properties to the regenerative template to encourage cell proliferation and differentiation. Several clinical studies have confirmed that direct or indirect electrical stimulation therapy greatly impacts the treatment of peripheral and central nerve injury. Nerve regeneration can be accelerated by the application of electrical stimulations of different methods and with varying parameters. For a long period, electrical stimulation along with conventional conductive polymers have played an important role in nerve tissue regeneration, due to their conductivity. However, the low biocompatibility of these materials has brought attention toward the need for the alternative of the conductive polymers. Carbon nanofillers (graphene, nanotubes, and their derivatives) have been showing promising results in bioimaging, biosensing, and composites, simultaneously, proving themselves as a prospective biomaterial in neural tissue repair and regeneration due to their excellent electrical and mechanical properties, alongside, biocompatibility. Therefore, carbon nanofiller-based scaffolds synchronized with electrical stimulation may lead to a breakthrough in the treatment of nerve injury. This review article focuses on the influence of the electrical properties of carbon-based material on nerve tissue engineering. In this article, we explicitly focus on the different methods to deliver electrical stimulation and the pathways involved in the differentiation of neuronal cells. Emphasis is given to the analysis of the suitable fabrication strategies of carbon-based neural scaffold and the way its interfaces interact with neurons for promoting neuronal differentiation. Furthermore, this review summarizes the ongoing advancements to augment the conductivity and biological activities (cell adhesion, proliferation, neural differentiation, neurite outgrowth), as well as to reduce the potential toxicity in conductive carbon nanofiller-based scaffolds.

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Neuronal differentiation of PC12 cells and mouse neural stem cells on carbon nanotube films

Cited by 6 publications

References 25 publications

Carbon Nanotube-Reinforced Poly(4-vinylaniline)/Polyaniline Bilayer-Grafted Bacterial Cellulose for Bioelectronic Applications

Carbon Nanotube-Reinforced Poly(4-vinylaniline)/Polyaniline Bilayer-Grafted Bacterial Cellulose for Bioelectronic Applications

Evaluation of in vitro neuronal networks for the study of spontaneous activity

Electrical Stimulation-Mediated Differentiation of Neural Cells on Conductive Carbon Nanofiller-Based Scaffold

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