Neuronal plasticity induced by neonatal monocular (and binocular) enucleation

Toldi, József; Fehér, O.; Wolff, Joachim R.

doi:10.1016/0301-0082(95)00038-0

Cited by 50 publications

(48 citation statements)

References 194 publications

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“…A remarkable number of publications have described changes in the organization of neuronal connections following neonatal CNS injury. In the visual system, retinal deafferentation at birth results in severe alterations of the afferent systems that project to SC superficial layers [19]. For example, removal of SC input in neonatal rodents results in an aberrant ipsilateral retinotectal projection [20][21][22][23][24], whereas retinal deafferentation in adults has no such effect [25][26][27].…”

Section: Differential Lesion Responses Of Neonatal and Adult Visual Cmentioning

confidence: 99%

The Experimental Manipulation of Visual Cortex Efferents

Gerrikagoitia¹,

Rienda²,

Martínez‐Millán³

2012

Visual Cortex - Current Status and Perspectives

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Section: Differential Lesion Responses Of Neonatal and Adult Visual Cmentioning

confidence: 99%

The Experimental Manipulation of Visual Cortex Efferents

Gerrikagoitia¹,

Rienda²,

Martínez‐Millán³

2012

Visual Cortex - Current Status and Perspectives

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“…It provides a valuable tool to study the different aspects of visual, cross-modal and developmental plasticity along the mammalian visual system [1][2][3][4] .…”

Section: Introductionmentioning

confidence: 99%

“…The enucleation model in the juvenile and adult visual system has proven to be valuable in understanding the development, plasticity and function of different visual centers [1][2][3][4] . The molecular, cellular and physiological consequences of this sensory deprivation can provide insights into how normal development is regulated and how established cortical circuits cope and change their structure and function in response to such an extensive alteration in experience.…”

Section: Introductionmentioning

confidence: 99%

“…Together, optic deafferentation results in different subcortical responses at the glial, structural and molecular level. Despite these subcortical effects, it does not necessarily implicate effects at the cortical level (BE) 1,17 enucleation.…”

Section: Introductionmentioning

confidence: 99%

“…It provides a valuable tool to study the different aspects of visual, cross-modal and developmental plasticity along the mammalian visual system [1][2][3][4] .Here, we demonstrate an elegant and straightforward technique for the removal of one or both eyes in the mouse, which is validated in mice of 20 days old up to adults. Briefly, a disinfected curved forceps is used to clamp the optic nerve behind the eye.…”

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confidence: 99%

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A Highly Reproducible and Straightforward Method to Perform <em>In Vivo</em> Ocular Enucleation in the Mouse after Eye Opening

Aerts

Nys

Arckens

2014

JoVE

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Enucleation or the surgical removal of an eye can generally be considered as a model for nerve deafferentation. It provides a valuable tool to study the different aspects of visual, cross-modal and developmental plasticity along the mammalian visual system [1][2][3][4] .Here, we demonstrate an elegant and straightforward technique for the removal of one or both eyes in the mouse, which is validated in mice of 20 days old up to adults. Briefly, a disinfected curved forceps is used to clamp the optic nerve behind the eye. Subsequently, circular movements are performed to constrict the optic nerve and remove the eyeball. The advantages of this technique are high reproducibility, minimal to no bleeding, rapid post-operative recovery and a very low learning threshold for the experimenter. Hence, a large amount of animals can be manipulated and processed with minimal amount of effort. The nature of the technique may induce slight damage to the retina during the procedure. This side effect makes this method less suitable as compared to Mahajan et al. (2011) 5 if the goal is to collect and analyze retinal tissue. Also, our method is limited to post-eye opening ages (mouse: P10 -13 onwards) since the eyeball needs to be displaced from the socket without removing the eyelids. The in vivo enucleation technique described in this manuscript has recently been successfully applied with minor modifications in rats and appears useful to study the afferent visual pathway of rodents in general.

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Manganese‐enhanced magnetic resonance imaging combined with electrophysiology in the evaluation of visual pathway in experimental rat models with monocular blindness

et al. 2017

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PurposeOur study aimed to explore the feasibility of manganese‐enhanced magnetic resonance imaging (MEMRI) combined with visual evoked potentials (VEP) and auditory evoked visual cortex responses (AVR) in evaluating for the establishment of visual/auditory compensatory pathways after monocular blindness.Materials and MethodsA total of 14 healthy neonatal male Sprague‐Dawley rats were randomly divided into two groups (n = 7 for Groups A and B). Right optic nerve (ON) transection was performed on the 7 rats of Group A to obtain a monocularly blind model, and the 7 rats of Group B were chosen as the control group. Four months later, 400 mmol MnCl2 was injected into the left eye in both groups via intravitreal injection. The changes in the visual pathways projected from the blind eye and the remaining eye in Group A and the normal eyes in Group B were compared to determine if new visual compensatory pathways were established. Additionally, VEP tests were performed to determine complete blindness, and AVR examinations were performed to help identify the generation of auditory compensatory function.ResultsThe VEP test indicated complete visual loss after ON transection. In the monocularly blind rats, the contrast‐to‐noise ratio (CNR) of ON, optic tract (OT), lateral geniculate nucleus (LGN), superior colliculus (SC), optic radiation (OR) and visual cortex (VC) of visual pathway projected from the left eye was significantly higher than that of the right pathway (p < .001). Moreover, the CNR of ON, OT, LGN, SC, OR and VC in the visual pathway projected from the left eye of monocularly blind rats was significantly lower than those of normal rats (p < .05). The AVR results revealed that the corresponding bilateral visual cortex in monocularly blind rats did not respond to the auditory stimulus or showed dissimilation with the low frequency.Conclusion MEMRI combined with electrophysiology, including VEP and AVR, may be potentially helpful in the evaluation of the possible generation of new visual/auditory compensatory pathways after monocular blindness.

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Neuronal plasticity induced by neonatal monocular (and binocular) enucleation

Cited by 50 publications

References 194 publications

The Experimental Manipulation of Visual Cortex Efferents

The Experimental Manipulation of Visual Cortex Efferents

A Highly Reproducible and Straightforward Method to Perform <em>In Vivo</em> Ocular Enucleation in the Mouse after Eye Opening

Manganese‐enhanced magnetic resonance imaging combined with electrophysiology in the evaluation of visual pathway in experimental rat models with monocular blindness

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