Neuronal protein GAP-43 is a member of novel group of brain acid-soluble proteins (BASPs)

Mosevitsky, Mark I.; Novitskaya, Vera; Plekhanov, A. Yu.; Skladchikova, Galina

doi:10.1016/0168-0102(94)90146-5

Cited by 26 publications

(19 citation statements)

References 36 publications

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“…Although these proteins do not share noticeable sequence homologies, they have a number of properties in common [47,65,[103][104][105]. All three proteins are characterized by high hydrophilicity, and all are subjected to N-terminal fatty acylation resulting in linkage to the plasma membrane preferentially in lipid rafts [8,97,106,107].…”

Section: Ncam and Growth-associated Proteins Basp1 And Marcksmentioning

confidence: 99%

Role of the Growth-Associated Protein GAP-43 in NCAM-Mediated Neurite Outgrowth

Korshunova

Mosevitsky

2009

Advances in Experimental Medicine and Biology

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During development, the neural cell adhesion molecule (NCAM) and growth-associated protein-43 (GAP-43) play key roles in neurite outgrowth and growth cone navigation [1][2][3]. These processes are vital for axonal growth and formation of highly organized neuronal nets in the brain. In adult organisms, NCAM and GAP-43 participate in the reorganization of synaptic contacts and learning and memory formation [4][5][6]. Enhanced synthesis of these proteins occurs during development and axonal regeneration. They are localized in detergent-resistant plasma membrane domains (rafts) [7][8][9][10][11]. NCAM was shown to deliver external signals to GAP-43 in growth cones, stimulating both GAP-43 phosphorylation on S41 and neurite outgrowth [1]. Therefore, numerous data suggest functional interdependence between NCAM and GAP-43. In this chapter, we discuss the interactions between GAP-43 and NCAM isoforms in the process of neurite outgrowth.

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Section: Ncam and Growth-associated Proteins Basp1 And Marcksmentioning

confidence: 99%

Role of the Growth-Associated Protein GAP-43 in NCAM-Mediated Neurite Outgrowth

Korshunova

Mosevitsky

2009

Advances in Experimental Medicine and Biology

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“…After 24 hr incubation at 37°C, 5% CO 2 , neurons were visualized using immunohistochemical staining for GAP-43 (Burry et al, 1991). Briefly, the cells were fixed for 30 min in 4% w/v paraformaldehyde in PBS, and then incubated in rabbit anti-GAP-43 antibodies (Mosevitsky et al, 1994) diluted 1:100 in PBS, 1% FCS, 0.1% BSA, 50 mM glycine, 0.02% NaN 3 , 0.2% saponin at 4°C overnight. After washing, biotinylated swine-anti-rabbit immunoglobulins (DAKO, Copenhagen, Denmark) diluted 1:100 in PBS, 1% BSA were added for 1 hr at room temperature.…”

Section: Co-culture Of Rat Hippocampal Neurons and Mouse Fibroblastoimentioning

confidence: 99%

Extracellular adenosine triphosphate affects neural cell adhesion molecule (NCAM)-mediated cell adhesion and neurite outgrowth

et al. 1999

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The neural cell adhesion molecule (NCAM) plays an important role in synaptic plasticity in embryonic and adult brain. Recently, it has been demonstrated that NCAM is capable of binding and hydrolyzing extracellular ATP. The purpose of the present study was to evaluate the role of extracellular ATP in NCAM-mediated cellular adhesion and neurite outgrowth. We here show that extracellularly added adenosine triphosphate (ATP) and its structural analogues, adenosine-5'-O-(3-thiothiophosphate), beta, gamma-methylenadenosine-5'-triphosphate, beta, gamma-imidoadenosine-5-triphosphate, and UTP, in varying degrees inhibited aggregation of hippocampal neurons. Rat glial BT4Cn cells are unable to aggregate when grown on agar but acquire this capacity when transfected with NCAM. However, addition of extracellular ATP to NCAM-transfected BT4Cn cells inhibited aggregation. Furthermore, neurite outgrowth from hippocampal neurons in cultures allowing NCAM-homophilic interactions was inhibited by addition of extracellular nucleotides. These findings indicate that NCAM-mediated adhesion may be modulated by extracellular ATP. Moreover, extracellularly added ATP stimulated neurite outgrowth from hippocampal neurons under conditions non-permissive for NCAM-homophilic interactions, and neurite outgrowth stimulated by extracellular ATP could be inhibited by a synthetic peptide corresponding to the so-called cell adhesion molecule homology domain (CHD) of the fibroblast growth factor receptor (FGFR) and by FGFR antibodies binding to this domain. Antibodies against the fibronectin type-III homology modules of NCAM, in which a putative site for ATP binding and hydrolysis is located, also abolished the neurite outgrowth-promoting effect of ATP. The non-hydrolyzable analogues of ATP all strongly inhibited neurite outgrowth. Our results indicate that extracellular ATP may be involved in synaptic plasticity through a modulation of NCAM-mediated adhesion and neurite outgrowth.

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“…The immunocy tochemical approach helps to reveal previously unknown proteins. In particular, BASP1 and MARCKS proteins first revealed in mammalian brain and involved in cytoskeletal structural reorganizations in axon growing cones were considered as specific only for nerve tissues (Mosevitsky et al, 1994;Mosevitsky, 2005;Zakharov and Mosevitsky, 2010). However, later it was recognized that they are of wider occurrence.…”

Section: The Role Of Chromatoid Bodies and Cytoskeleton Inmentioning

confidence: 97%

The role of chromatoid bodies and cytoskeleton in differentiation of rat spermatozoids

2012

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An ultrastructural and immunocytochemical study of rat male germ cells at different stages of development has been carried out. Investigation of morphological changes of spermatogenic cells showed the presence of close associations between chromatoid bodies (CBs) and other cell organelles, particularly with the nucleus and Golgi apparatus. In addition, a connection of manchette noncentosomal microtubules (MTs) with spermatid perinuclear ring plasma membrane (PM) in the zone of adhesion intercellular contact, zonula adhaerens (ZA), was revealed. These results, as well as the available literary data, make it possible to analyze expected pathways of noncentrosomal MT nucleation in the late spermatids. It is possible to suggest that noncentorosomal MT are nucleated on the sites of perinuclear ring ZA. The immunocytochemical anal ysis revealed two novel proteins for these cells: BASP1 and MARCKS. It was shown that these proteins were present in CBs in early spermatids. During spermatozoid differentiation, these proteins are located along the outer dense fibers (ODFs) of the sperm tail. BASP1 and MARCKS are believed to be involved in the processes of calcium accumulation in CBs and ODFs. Calcium ions seem to play a significant role in RNA processing and protein synthesis in spermatids. Calcium is also necessary for sperm mobility defined mainly by ODFs.

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Neuronal protein GAP-43 is a member of novel group of brain acid-soluble proteins (BASPs)

Cited by 26 publications

References 36 publications

Role of the Growth-Associated Protein GAP-43 in NCAM-Mediated Neurite Outgrowth

Role of the Growth-Associated Protein GAP-43 in NCAM-Mediated Neurite Outgrowth

Extracellular adenosine triphosphate affects neural cell adhesion molecule (NCAM)-mediated cell adhesion and neurite outgrowth

The role of chromatoid bodies and cytoskeleton in differentiation of rat spermatozoids

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