Neuropeptide B promotes differentiation of rodent white preadipocytes into mature adipocytes

The Effects of Neuropeptide B on Proliferation and Differentiation of Porcine White Preadipocytes into Mature Adipocytes

“…We identified NPB as well as both of its receptors in porcine preadipocytes. This observation is consistent with our previous study, in which we detected the expression of NPB as well as NPBWR1 in rodent white fat precursor cells (rat primary preadipocytes and 3T3-L1 cells) [ 27 ]. Surprisingly, in our current study, we found that protein production, but not the mRNA expression of NPBWR1 and NPBWR2, was downregulated during the differentiation process.…”

“…Our results show that NPB stimulated the proliferation of preadipocytes. Interestingly, in our previous study, NPB failed to affect the proliferation of rat preadipocytes [ 27 ]. It cannot be excluded that these discrepancies resulted from the different species (rat vs. pig).…”

“…Nevertheless, it is important to note that the effects of NPB on the expression of these above-mentioned genes were observed in cells differentiated for one day only. Notably, in our previous study in rat preadipocytes, NPB was more effective in stimulating the differentiation process in cells differentiated for one or three days, in comparison with cells differentiated for one week [ 27 ]. Therefore, these data suggest that NPB may mainly be involved in early stages of the differentiation process.…”

“…Recently, we found that NPB is able to promote ERK1/2 phosphorylation in insulin-producing INS-1E cells [ 10 ]. Furthermore, we reported that NPB induces the phosphorylation of p38 kinase in rat white and brown primary preadipocytes [ 14 , 27 ]. As indicated in the introduction, both protein kinases, p-38 and ERK1/2, are involved in controlling adipogenesis [ 42 , 43 , 44 ], so we therefore studied whether NPB is able to modulate the phosphorylation of these protein kinases in porcine preadipocytes.…”

“…MAPKs encompass three main pathways: ERK1/2-, JNK- and p38-dependent [ 26 ]. We previously demonstrated that NPB can potentiate the differentiation of white rodent preadipocytes into adipocytes via the p38-dependent mechanism [ 27 ]. The p38 kinases are involved in processing cellular responses to nearly all stress factors [ 28 ].…”