Neuropeptide reporter assay for serum, capillary blood and blood cards

Schreiber, Ulrich; Engl, Christian; Bayer, Malte; König, Simone

doi:10.1016/j.mex.2020.100985

Cited by 6 publications

(2 citation statements)

References 5 publications

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“…This question can only be answered by dedicated clinical studies. They have potential, because the BK assay is a low-cost/low-tech procedure based on thin-layer chromatography [4] that can be performed in any laboratory and only requires small samples [39]. If its results truly indicate Long COVID candidates, it might be a valuable tool to speed up patient diagnosis and therapy.…”

Section: Discussionmentioning

confidence: 99%

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The Renin–Angiotensin System in COVID-19: Can Long COVID Be Predicted?

König

Tepasse

2023

Life

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(1) Background: Co-morbidities such as hypertension and cardiovascular disease are major risk factors for severe COVID-19. The renin–angiotensin system (RAS) is critically involved in their pathophysiology and is counter-balanced by both angiotensin-converting enzyme 2 (ACE2), the functional receptor of SARS-CoV-2, and the kallikrein–kinin system (KKS). Considerable research interest with respect to COVID-19 treatment is currently being directed towards the components of these systems. In earlier studies, we noticed significantly reduced carboxypeptidase N (CPN, KKS member) activity and excessive angiotensin-converting enzyme (ACE, RAS member) activity in the sera of both hospitalized COVID-19 patients and a subgroup of convalescent patients. The data had been obtained using labeled bradykinin (BK) as a reporter peptide, which is a target of both CPN and ACE. The data were supplemented with mass-spectrometry-based serum proteomic analysis. Here, we hypothesize that the degree of BK serum degradation could be indicative of Long COVID. (2) Review and Discussion: The recent literature is briefly reviewed. The fact that the levels of the BK serum degradation products did not reach normal concentrations in almost half of the patients during convalescences could have been partially due to a dysregulated RAS. (3) Conclusions: Standard tests for routine patient care in Long COVID come often back normal. We suggest that the measurement of selected members of the RAS such as ACE and angiotensin II or the use of our BK degradation assay could identify Long COVID candidates. Clinical studies are required to test this hypothesis.

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Section: Discussionmentioning

confidence: 99%

“…The fact that ACE and CPN activity remains imbalanced in some individuals may assist research into Long COVID pathophysiology. Our low-tech assay can be of assistance in identifying these persons as it only requires a drop of capillary blood or serum [39].…”

Section: Is Reduced Bk Serum Degradation In Convalescent Patients Ind...mentioning

confidence: 99%