Neuropeptide Y family receptors Y1 and Y2 from sea lamprey, Petromyzon marinus

Xu, Bo; Lagman, David; Sundström, Görel; Larhammar, Dan

doi:10.1016/j.ygcen.2015.08.005

Cited by 4 publications

(1 citation statement)

References 52 publications

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“…NPY and its receptors have been studied extensively in the past few decades. The NPY receptor family eventually produced seven receptor subtypes named NPY1R, NPY2R, NPY4R, NPY5R, NPY6R, NPY7R, and NPY8R through two whole-genome duplications in the early stage of vertebrate evolution (Larsson et al, 2009;Sundström et al, 2013;Xu et al, 2015). NPY receptors have different receptor gene deletions in mammals and teleost fish, in this study, the phylogenetic tree showed that medaka npy2r is homologous to human and mouse npy2r.…”

Section: Discussionmentioning

confidence: 54%

Neuropeptide Y receptor Y2 (npy2r) deficiency reduces anxiety and increases food intake in Japanese medaka (Oryzias latipes)

Lu,

Jia,

et al. 2023

Front. Cell Dev. Biol.

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Neuropeptide Y receptor Y2 (npy2r) is an important receptor gene involved in anxiety and feeding regulation in mammals. Since NPY receptors have different receptor gene deletions in mammals and teleost fish, it is not clear whether npy2r has the similar function in fish as in mammals. In this study, we used the CRISPR/Cas9 system to establish npy2r-deficient medaka (Oryzias latipes). Unexpectedly, the deletion of npy2r resulted in the npy2r+/− medaka were all-male, therefore, npy2r homozygous mutant lines could not be established. The deletion of npy2r increased the food intake in medaka, and the expression levels of appetite stimulating genes (agrp, npy) increased significantly, while the expression levels of anorexia factors (cck, pomc) decreased significantly. Moreover, the absence of npy2r significantly increased the total length and body weight of medaka. The mirror test and open field test showed that npy2r+/− medaka improved sociability and reduced anxiety-like behavior, qRT-PCR analysis showed that the expression levels of anxiety related genes (th1, th2, gr1, gr2, and mr) in npy2r+/− medaka were significantly decreased. So far, this is the first npy2r gene knockout model established in fish and demonstrates that npy2r plays an important role in the regulation of reproduction, feeding and anxiety in fish.

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Section: Discussionmentioning

confidence: 54%