Neuropharmacological Characterization of Dioclea altissima Seed Lectin (DAL) in Mice: Evidence of Anxiolytic-like Effect Mediated by Serotonergic, GABAergic Receptors and NO Pathway

Araújo, João Ronielly Campêlo; Campos, Adriana Rolim; Damasceno, Marina de Barros Mamede Vidal; Santos, Sacha Aubrey Alves Rodrigues; Ferreira, Maria Kueirislene Amâncio; Moreira, Renato de Azevedo; Monteiro-Moreira, Ana Cristina de Oliveira

doi:10.2174/1381612826666200331093207

Cited by 7 publications

(3 citation statements)

References 43 publications

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“…Animal head-dipping behavior remains closely correlated with their psychological response. Gamma amino-butyric acid (GABA) can modulate the central nervous system through the voltage-gated Ca 2+ channel or chlorine conductance [40][41][42][43][44][45][46][47][48][49][50]. It was revealed that the animals' head-dipping behavior is clearly relevant to their mental state, and this test is used to investigate anxiety-related activities.…”

Section: Discussionmentioning

confidence: 99%

Citrus limon L. (lemon) seed extract shows neuro-modulatory activity in an in vivo thiopental-sodium sleep model by reducing the sleep onset and enhancing the sleep duration

Rahman¹,

Islam²,

Parvez³

et al. 2022

J. Integr. Neurosci.

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Citrus limon L. is an ingenious alternative medication and has a broad scope in managing several health conditions as part of natural remedies. Recently, medicinal plants have witnessed incredible consideration worldwide in the field of neuroscience for remedial intervention. The present work has investigated the phytochemical compounds and neuropharmacological potential of the seed extract of Citrus limon as a step to partially validate its formulations as nutraceuticals using an in vivo model. Diverse phytochemical groups such as alkaloids, glycosides, lavonoids, tannins, gums, saponins, steroids were qualitatively identified through colorimetric methods utilizing standard compounds. The neuropharmacological properties were studied in Swiss albino mice with the sleep time induced by thiopental sodium taken as an end-point, in standard hole cross, hole board, and open-field experiments at varying doses of 50 and 100 mg/kg body weight. Phytochemical screening showed that alkaloids, lavonoids, saponins, tannins, steroids, and glycosides are present in the aqueous extract of the seed. The extracts demonstrated a significant reduction in sleep onset and enhanced the sleep duration in a dose-dependent manner in thiopental sodium-induced sleeping time, along with a marked decrease in unconstrained locomotors and explorative properties in both hole cross and open field tests. Moreover, in the hole board study, the extracts minimized the count of head dips observed in the treated mice. The results shown in this study demonstrate that Citrus limon extracts have neuropharmacological properties that can be further examined for their potential role as an adjuvant with conventional medications or nutraceuticals.

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Section: Discussionmentioning

confidence: 99%

Citrus limon L. (lemon) seed extract shows neuro-modulatory activity in an in vivo thiopental-sodium sleep model by reducing the sleep onset and enhancing the sleep duration

Rahman¹,

Islam²,

Parvez³

et al. 2022

J. Integr. Neurosci.

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“…A large amount of flavonoids in M. oleifera leaves can interact with GABA receptors like a benzodiazepine molecule [53,64]. Additionally, evidence has suggested the possible binding of plant lectins to GABAergic receptors [65]. Together, both constituents of MoLE may be responsible for improving the inhibitory signaling of GABA receptors.…”

Section: Discussionmentioning

confidence: 99%

Saline Extract from Moringa oleifera Leaves Has Antidepressant and Anxiolytic Effects in Mouse Models

Fidelis,

Alves,

Patriota

et al. 2024

Nutraceuticals

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Plant extracts can be therapeutic alternatives for depression and anxiety. However, some plant-derived preparations can also be toxic. Moringa oleifera leaves are used in human nutrition due to their high nutritional value and antioxidant activity. This study investigated a saline extract from M. oleifera leaves (MoLE) for secondary metabolites, proteins, cytotoxicity, hemolytic activity, in vivo acute oral toxicity, and neurobehavioral effects. MoLE contains flavonoids (rutin and vitexin), lectin, and a trypsin inhibitor. It is neither cytotoxic nor hemolytic for human cells and did not present acute oral toxicity (2000 mg/kg) to mice. The elevated plus maze test showed that MoLE (500, 1000, and 2000 mg/kg, p.o.) significantly increased the number of entries as well as the time spent by mice in open arms, while it decreased the number of entries and the time spent in closed arms when compared to the control. MoLE (500, 1000, and 2000 mg/kg, p.o.) reduced immobility time of mice in the tail suspension and forced swimming tests, compared to the control. The anxiolytic-like effect of MoLE is possibly mediated by a GABA mimetic action once it is prevented by pre-treatment with flumazenil. The present study demonstrated that MoLE has antidepressant and anxiolytic effects in mice and is a promising herbal medicine.

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“…in Swiss mice. Its effect was mediated by the serotonergic and GABAergic systems as well as NO pathway [73].…”

Section: Ftl (Frutalin) α-D-galactose-binding Lectin Frommentioning

confidence: 99%

Can Plant Lectins be Alternatives to Treat Anxiety and Depressive Disorders?

Lima

Patriota

Albuquerque

et al. 2020

AIR

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Depression and anxiety disorder are the most common mental disorders worldwide and their treatments are combinations of pharmacological and psychotherapeutic approaches. Depression treatment depends largely on a pharmacotherapy that improves the transmission of monoamines in the brain. However, the drugs available have adverse reactions and do not contemplate positively all patients, which stimulates scientific research that seeks new molecules, including from natural sources. Lectins are proteins capable of binding reversibly and non-covalently to specific sugars. For example, it has been reported the antimicrobial, antitumor, antiparasitic, anti-inflammatory and antinociceptive activities of lectins. The ability of some lectins in modulating nociception and inflammation stimulated studies on the possible effects on processes that share some pathways and molecular agents, like depression and anxiety. Lectins isolated from plants showed antidepressant effects, which were demonstrated to be linked to activation of serotonergic, adrenergic, and dopaminergic systems as well as to inhibition of the glutamatergic system and L-arginine–NO–cGMP pathway. In view of their immunomodulatory properties, it is also suggested that lectins can ameliorate the inflammatory framework associated with depression. Anxiolytic effects were also reported and associated with modulation of GABAergic mechanisms, serotonergic system, and NO pathway. It should be taken in account that some lectins induced depressive-like behavior, associated with an neuroinflammatory action, as well anxiogenic action. Thus, it is important to use combinations of batteries for testing anxiety, depression, despair, and anhedonia behaviors in the studies with lectins. The mechanisms by which lectins exactly modulate depression or anxiety frameworks are still unclear but important windows had already been open by researchers and preclinical studies with lectins have indicated these proteins as candidates for alternative or complementary agents in therapies of depression and anxiety disorder.

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Neuropharmacological Characterization of Dioclea altissima Seed Lectin (DAL) in Mice: Evidence of Anxiolytic-like Effect Mediated by Serotonergic, GABAergic Receptors and NO Pathway

Cited by 7 publications

References 43 publications

Citrus limon L. (lemon) seed extract shows neuro-modulatory activity in an in vivo thiopental-sodium sleep model by reducing the sleep onset and enhancing the sleep duration

Citrus limon L. (lemon) seed extract shows neuro-modulatory activity in an in vivo thiopental-sodium sleep model by reducing the sleep onset and enhancing the sleep duration

Saline Extract from Moringa oleifera Leaves Has Antidepressant and Anxiolytic Effects in Mouse Models

Can Plant Lectins be Alternatives to Treat Anxiety and Depressive Disorders?

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