Neurophenotyping of Adult Zebrafish Using the Light/Dark Box Paradigm

Stewart, Adam; Maximino, Caio; Brito, Thiago Marques; Herculano, Anderson Manoel; Gouvêia, Amauri; Morato, Sílvio; Cachat, Jonathan; Gaikwad, Siddharth; Elegante, Marco; Hart, Peter C.; Kalueff, Allan V.

doi:10.1007/978-1-60761-953-6_13

Cited by 54 publications

(45 citation statements)

References 23 publications

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“…In general, the scototaxic response of adult zebrafish to the light/dark box test in this study was consistent with the behavioural response previously observed for this paradigm [18]. In mammals, highly anxious individuals often display a subordinate status [26].…”

Section: Discussionsupporting

confidence: 91%

Acute embryonic anoxia exposure favours the development of a dominant and aggressive phenotype in adult zebrafish

2017

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Eutrophication and climate change are increasing the incidence of severe hypoxia in fish nursery habitats, yet the programming effects of hypoxia on stress responsiveness in later life are poorly understood. In this study, to investigate whether early hypoxia alters the developmental trajectory of the stress response, zebrafish embryos were exposed to 4 h of anoxia at 36 h post-fertilization and reared to adults when the responses to secondary stressors were assessed. While embryonic anoxia did not affect basal cortisol levels or the cortisol response to hypoxia in later life, it had a marked effect on the responses to a social stressor. In dyadic social interactions, adults derived from embryonic anoxia initiated more chases, bit more often, entered fewer freezes and had lower cortisol levels. Adults derived from embryonic anoxia also performed more bites towards their mirror image, had lower gonadal aromatase gene expression and had higher testosterone levels. We conclude that acute embryonic anoxia has long-lasting consequences for the hormonal and behavioural responses to social interactions in zebrafish. Specifically, we demonstrate that acute embryonic anoxia favours the development of a dominant and aggressive phenotype, and that a disruption in sex steroid production may contribute to the programming effects of environmental hypoxia.

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Section: Discussionsupporting

confidence: 91%

Acute embryonic anoxia exposure favours the development of a dominant and aggressive phenotype in adult zebrafish

2017

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“…Zebrafish can display atypical behavioral phenotypes, and different strains of zebrafish may have different levels of baseline anxiety. Further, variations in response can also be seen between wild type strains, with some fish avoiding ambient light (Stewart et al, 2011b).…”

Section: Accepted Manuscriptmentioning

confidence: 96%

Pharmacological study of the light/dark preference test in zebrafish (Danio rerio): Waterborne administration

Magno

Fontes

Gonçalves

et al. 2015

Pharmacology Biochemistry and Behavior

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“…Zebrafish have been used to study the fundamental mechanisms governing the exhibition of emotional patterns [13,14], individual response to alcohol and drugs of abuse [15][16][17], and higher order brain functions, such as memory and learning [18]. Zebrafish behaviour is typically measured from locomotory patterns [19,20], which are detected in the form of changes in speed and turn rate [5,6]; these patterns are annotated via human observation [21] or classified on the basis of sequences of trajectory data obtained using vision-based tracking [22,23].…”

Section: Introductionmentioning

confidence: 99%

A jump persistent turning walker to model zebrafish locomotion

Mwaffo

Anderson

Butail

et al. 2015

J. R. Soc. Interface.

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Zebrafish are gaining momentum as a laboratory animal species for the investigation of several functional and dysfunctional biological processes. Mathematical models of zebrafish behaviour are expected to considerably aid in the design of hypothesis-driven studies by enabling preliminary in silico tests that can be used to infer possible experimental outcomes without the use of zebrafish. This study is motivated by observations of sudden, drastic changes in zebrafish locomotion in the form of large deviations in turn rate. We demonstrate that such deviations can be captured through a stochastic mean reverting jump diffusion model, a process that is commonly used in financial engineering to describe large changes in the price of an asset. The jump process-based model is validated on trajectory data of adult subjects swimming in a shallow circular tank obtained from an overhead camera. Through statistical comparison of the empirical distribution of the turn rate against theoretical predictions, we demonstrate the feasibility of describing zebrafish as a jump persistent turning walker. The critical role of the jump term is assessed through comparison with a simplified mean reversion diffusion model, which does not allow for describing the heavy-tailed distributions observed in the fish turn rate.

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Neurophenotyping of Adult Zebrafish Using the Light/Dark Box Paradigm

Cited by 54 publications

References 23 publications

Acute embryonic anoxia exposure favours the development of a dominant and aggressive phenotype in adult zebrafish

Acute embryonic anoxia exposure favours the development of a dominant and aggressive phenotype in adult zebrafish

Pharmacological study of the light/dark preference test in zebrafish (Danio rerio): Waterborne administration

A jump persistent turning walker to model zebrafish locomotion

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