Neuropilin 1 Mediates Keratinocyte Growth Factor Signaling in Adipose-Derived Stem Cells: Potential Involvement in Adipogenesis

Ceccarelli, Simona; Nodale, Cristina; Vescarelli, Enrica; Pontecorvi, Paola; Manganelli, Valeria; Casella, Giovanni; Onesti, Maria Giuseppina; Sorice, Maurizio; Romano, Ferdinando; Angeloni, Antonio; Marchese, Cinzia

doi:10.1155/2018/1075156

Cited by 25 publications

(24 citation statements)

References 54 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In mice, we showed that Sema3s acting via Nrp2 direct the development of Pomc projections from the arcuate to the paraventricular nucleus of the hypothalamus. A role for Nrp1-mediated signaling in pre-adipocyte differentiation has been demonstrated ( Ceccarelli et al., 2018 ). In zebrafish, plxnd1 has been shown to regulate adipose tissue growth by affecting the formation of the extracellular matrix ( Minchin et al., 2015 ), indicating that Semaphorin 3 signaling may affect non-neural mechanisms that contribute to adiposity.…”

Section: Discussionmentioning

confidence: 99%

Human Semaphorin 3 Variants Link Melanocortin Circuit Development and Energy Balance

Klaauw

Croizier

Oliveira

et al. 2019

Cell

106

View full text Add to dashboard Cite

SummaryHypothalamic melanocortin neurons play a pivotal role in weight regulation. Here, we examined the contribution of Semaphorin 3 (SEMA3) signaling to the development of these circuits. In genetic studies, we found 40 rare variants in SEMA3A-G and their receptors (PLXNA1-4; NRP1-2) in 573 severely obese individuals; variants disrupted secretion and/or signaling through multiple molecular mechanisms. Rare variants in this set of genes were significantly enriched in 982 severely obese cases compared to 4,449 controls. In a zebrafish mutagenesis screen, deletion of 7 genes in this pathway led to increased somatic growth and/or adiposity demonstrating that disruption of Semaphorin 3 signaling perturbs energy homeostasis. In mice, deletion of the Neuropilin-2 receptor in Pro-opiomelanocortin neurons disrupted their projections from the arcuate to the paraventricular nucleus, reduced energy expenditure, and caused weight gain. Cumulatively, these studies demonstrate that SEMA3-mediated signaling drives the development of hypothalamic melanocortin circuits involved in energy homeostasis.

show abstract

Section: Discussionmentioning

confidence: 99%

Human Semaphorin 3 Variants Link Melanocortin Circuit Development and Energy Balance

Klaauw

Croizier

Oliveira

et al. 2019

Cell

106

View full text Add to dashboard Cite

show abstract

“…As for the external stimuli, it has been demonstrated that the presence of growth factors in the culture medium could enhance ASCs differentiation (Ceccarelli et al, 2018). Likewise, preconditioning of ASCs with cytokines or other bioactive molecules represents one of the main approaches to boost ASCs immune regulatory functions before their therapeutic administration.…”

Section: Approaches For Optimization Of Ascs Immunomodulatory Efficacymentioning

confidence: 99%

Immunomodulatory Effect of Adipose-Derived Stem Cells: The Cutting Edge of Clinical Application

Ceccarelli

Pontecorvi

Anastasiadou

et al. 2020

Front. Cell Dev. Biol.

Self Cite

148

102

View full text Add to dashboard Cite

Adipose-derived stem cells (ASCs) represent a promising tool for soft tissue engineering as well as for clinical treatment of inflammatory and autoimmune pathologies. The well-characterized multi-differentiation potential and self-renewal properties of ASCs are coupled with their immunomodulatory ability in providing therapeutic efficacy. Yet, their impact in immune or inflammatory disorders might rely both on cell contactdependent mechanisms and paracrine effects, resulting in the release of various soluble factors that regulate immune cells functions. Despite the widespread use of ASCs in clinical trials addressing several pathologies, the pathophysiological mechanisms at the basis of their clinical use have been not yet fully investigated. In particular, a thorough analysis of ASC immunomodulatory potential is mandatory. Here we explore such molecular mechanisms involved in ASC immunomodulatory properties, emphasizing the relevance of the milieu composition. We review the potential clinical use of ASC secretome as a mediator for immunomodulation, with a focus on in vitro and in vivo environmental conditions affecting clinical outcome. We describe some potential strategies for optimization of ASCs immunomodulatory capacity in clinical settings, which act either on adult stem cells gene expression and local microenvironment. Finally, we discuss the limitations of both allogeneic and autologous ASC use, highlighting the issues to be fixed in order to significantly improve the efficacy of ASC-based cell therapy.

show abstract

“…Grun and colleagues showed that VEGF-A/NRP1 signalling enhances epidermal cancer stem cell spheroid formation, angiogenic potential, invasion, and migration by activating MAPK signalling [29] . In addition, Ceccarelli et al reported that NRP1 is responsible for keratinocyte growth factordependent ERK and p38 MAPK pathway activation in human adipogenesis [30] . Therefore, we hypothesized that NRP1 may have a significant impact on MAPK pathway activation in BC cells.…”

Section: Discussionmentioning

confidence: 99%

The NRP1 gene regulates proliferation, apoptosis, migration, and invasion in T24 and 5637 bladder cancer cells

Han

Dong

Hao

et al. 2020

Preprint

View full text Add to dashboard Cite

Background Bladder urothelial carcinoma (BC) is a fatal invasive malignancy and the most common malignancy of the urinary system. In the current study, we investigate the function and mechanisms of Neuropilin-1 (NRP1), the co-receptor for vascular endothelial growth factor, in BC pathogenesis and progression. Methods The expression of NRP1 was assessed in several BC cell lines. Additionally, the biological function of NRP1 in proliferation, apoptosis, angiogenesis, migration, and invasion of BC were validated in vitro by silencing NRP1. Moreover, gene expression profiling chip analysis was conducted, and the related signalling pathways were confirmed by Western blot to reveal the potential molecular mechanisms by which NRP1 promotes the malignant progression of BC. Results Overexpression of NRP1 was observed in several human BC cell lines. NRP1 knockdown inhibited cell proliferation, promoted apoptosis, and decreased angiogenesis, migration, and invasion in T24 and 5637 human BC cells. Microarray analysis results indicated that the expression of NRP1 was correlated with the levels of cyclin dependent kinase (CDK) 4, baculoviral IAP repeat containing 3, Cyclin E 2, CDK2, and AP-1 transcription factor subunit in BC. We also demonstrated that the biological function of NRP1was associated with activation of the mitogen-activated protein kinase (MAPK) signalling pathway. Conclusions Our findings provide evidence that NRP1, as a potential tumour promoter, contributes to the metastasis and invasion of BC, which is associated with the activation of the MAPK pathway. Targeting NRP1 has the potential to become a new therapeutic strategy to benefit more patients with BC or other cancers.

show abstract

Neuropilin 1 Mediates Keratinocyte Growth Factor Signaling in Adipose-Derived Stem Cells: Potential Involvement in Adipogenesis

Cited by 25 publications

References 54 publications

Human Semaphorin 3 Variants Link Melanocortin Circuit Development and Energy Balance

Human Semaphorin 3 Variants Link Melanocortin Circuit Development and Energy Balance

Immunomodulatory Effect of Adipose-Derived Stem Cells: The Cutting Edge of Clinical Application

The NRP1 gene regulates proliferation, apoptosis, migration, and invasion in T24 and 5637 bladder cancer cells

Contact Info

Product

Resources

About