Neuroprotective action of aspirin on Paraquat intoxication in on Drosophila melanogaster

Abstract

Acetylsalicylic acid or aspirin is the most widely used drug globally for its anti-inflammatory characteristics, although little is known about its actions on the central nervous system (CNS). We investigated aspirin's potential neuroprotective effects against paraquat-induced neurotoxicity (PQ) in the present study. Adult male wild-type flies were exposed to a diet containing PQ (3 mM) and/or aspirin (1 μM; 5 μM; 10 μM). Flies fed with PQ reduced locomotion and increased mortality. PQ-induced neurotoxicity has also been associated with a marked decrease in acetylcholinesterase (AChE) activity and lipid peroxidation. Co-exposure to aspirin (5 μM) increased survival, improved motor performance, increased AChE activity, and decreased lipid peroxidation. Our results suggest aspirin's neuroprotective effects, probably due to its lysosomal action and antioxidant characteristics. Thus, we demonstrate that the Drosophila melanogaster model can elucidate basic aspirin mechanisms to assist the evaluations carried out in higher animals to minimize the neurodegenerative effects caused by diseases such as Parkinson's and Alzheimer's.

Piranhea trifoliata extracts ameliorate muscular decline in Drosophila melanogaster exposed to Paraquat

“…Our results showed PQ exposition increased the activity of AChE, improved the acetylcholine hydrolysis, and reduced muscular contraction in the muscle cell membranes. Diet supplemented with the P. trifoliata extracts showed a protective effect in AChE activity against damage caused by PQ intoxication, as reported by Carmo et al (2021).…”

“…In D. melanogaster , the exposition of aspirin (Carmo et al, 2021), omega‐3 (de Oliveira Souza et al, 2017; Souza et al, 2019), saffron methanolic extract (Rao et al, 2016), and Decalepis hamiltonii extracts (Jahromi et al, 2013) showed a protective effect against potential neuronal or muscular damage.…”

“…Studies have shown that 5‐days PQ ingestion has significant toxic effects on locomotor ability (climbing test), suggesting toxicity in the thoracic musculature of D. melanogaster (Carmo et al, 2021; de Oliveira Souza et al, 2017). In this work, D. melanogaster (wild type, Canton Special) was exposed to PQ for 10 days, affecting the dopaminergic and cholinergic systems (Lovejoy & Fiumera, 2019).…”

Protective effect of Amazonian Himatanthus sucuuba extracts in Drosophila melanogaster exposed to Paraquat

“…Research, Society and Development, v. 11, n. 17, e148111738931, 2022 (CC BY 4.0) | ISSN 2525-3409 | DOI: http://dx.doi.org/10.33448/rsd-v11i17.38931 12 Additionality, our results showed PQ exposition increased the activity of acetylcholinesterase (AChE), improved the acetylcholine hydrolysis, and reduced muscular contraction in the muscle cell membranes. Diet supplemented with H. sucuuba demonstrated a protective effect in AChE activity against damage caused by PQ intoxication, as reported by Carmo et al (2021).…”