Neuroprotective and anti-oxidant effects of caffeic acid isolated from Erigeron annuus leaf

Abstract

BackgroundSince oxidative stress has been implicated in a neurodegenerative disease such as Alzheimer's disease (AD), natural antioxidants are promising candidates of chemopreventive agents. This study examines antioxidant and neuronal cell protective effects of various fractions of the methanolic extract of Erigeron annuus leaf and identifies active compounds of the extract.MethodsAntioxidant activities of the fractions from Erigeron annuus leaf were examined with [2,2-azino-bis(3-ethylbenz thiazoline-6-sulfonic acid diammonium salt)] (ABTS) and ferric reducing antioxidant power (FRAP) assays. Neuroprotective effect of caffeic acid under oxidative stress induced by H2O2 was investigated with [3-(4,5-dimethythiazol-2-yl)-2,5-diphenyl tetrazolium bromide] (MTT) and lactate dehydrogenase (LDH) assays.ResultsThis study demonstrated that butanol fraction had the highest antioxidant activity among all solvent fractions from methanolic extract E. annuus leaf. Butanol fraction had the highest total phenolic contents (396.49 mg of GAE/g). Caffeic acid, an isolated active compound from butanol fraction, showed dose-dependent in vitro antioxidant activity. Moreover, neuronal cell protection against oxidative stress induced cytotoxicity was also demonstrated.ConclusionErigeron annuus leaf extracts containing caffeic acid as an active compound have antioxidative and neuroprotective effects on neuronal cells.

Butylated caffeic acid: An efficient novel antioxidant

“…The 1 H and 13 C chemical shifts of compound BCA agree well with its chemical structure, which is compared with the spectra data for caffeic acid reported in the literature (Jeong et al, 2011).…”

Methanol extracts from Cystoseira tamariscifolia and Cystoseira nodicaulis are able to inhibit cholinesterases and protect a human dopaminergic cell line from hydrogen peroxide-induced cytotoxicity

“…The highest hydroxycinnamic acids content in C. tamariscifolia could thus explain its higher RSA, when compared with C. nodicaulis. Additionally, there is evidence that different hydroxycinnamic acids can protect neuronal cells against oxidative damage in in vitro models (Pavlica & Gebhardt 2005;Nakajima et al 2009;Jeong et al 2011;Garrido et al 2012). Moreover, in vivo studies in rats and mice suggest that those compounds can be effective neuroprotective agents (Cheng et al 2008;Tsai et al 2011).…”

Non-toxic Salvia sclareoides Brot. extracts as a source of functional food ingredients: Phenolic profile, antioxidant activity and prion binding properties

“…The antioxidant, anti-inflammatory and immunostimulatory ferulic acid (Chao & Lin, 2011) promotes degeneration of recombinant beta-amyloid peptide, as described by Picone et al (2009). Also caffeic acid has neuroprotective effects against A beta-induced toxicity by the inhibition of calcium influx and tau phosphorylation (Sul et al, 2009), and protects neuronal cells against oxidative stress induced cytotoxicity (Jeong et al, 2011). Both caffeic and coumaric acids are capable of inducing neuroprotective effects in Parkinson's disease (Vauzour, Corona, & Spencer, 2010) to a similar extent to that seen with the flavonoids (+)-catechin and quercetin, also present in the n-butanol extract (Martin, Gonzalez-Burgos, Carretero, & Gomez-Serranillos, 2011).…”