Neuroprotective and free radical scavenging activities of phenolic compounds fromHovenia dulcis

Abstract

The EtOAc-soluble fraction from a methanolic extract of Hovenia dulcis Thunb. exhibited neuroprotective activity against glutamate-induced neurotoxicity in mouse hippocampal HT22 cells. The neuroprotective activity-guided isolation resulted in 8 phenolic compounds (1-8), such as vanillic acid (1), ferulic acid (2), 3,5-dihydroxystilbene (3), (+)-aromadendrin (4), methyl vanillate (5), (-)-catechin (6), 2,3,4-trihydrobenzoic acid (7), and (+)-afzelechin (8). Among these, compounds 6 and 8 had a neuroprotective effect on the glutamate-induced neurotoxicity in HT22 cells. Furthermore, compound 6 had a DPPH free radical scavenging effect with an IC50 value of 57.7 microM, and a superoxide anion radical scavenging effect with an IC50 value of 8.0 microM. Both compounds 6 and 8 had ABTS cation radical scavenging effects with IC50 values of 7.8 microM and 23.7 microM, respectively. These results suggest that compounds 6 and 8 could be neuroprotectants owing to their free radical scavenging activities.

In vitro evaluation of the cytotoxic and trypanocidal activities of Ampelozizyphus amazonicus (Rhamnaceae)

“…In addition, triterpenes, compounds such as phenols, saponins, peptide alkaloids, and others have been identified in Rhamnaceae species (5,(25)(26)(27)(28).…”

Semen Hoveniae extract protects against acute alcohol-induced liver injury in mice

“…Semen Hoveniae, the seed of the Hovenia dulcis Thunb. (Rhamnaceae), was found to show neuroprotective (Li et al, 2005) and hepatoprotective (Hase et al, 1997) effects. However, the protective activity of Semen Hoveniae against acute alcohol-induced liver injury has not been studied, as far as we know.…”

Elaboration and characterization of Japanese Raisin Tree (Hovenia dulcis Thumb.) pseudofruits fermented alcoholic beverage

“…The presence of vanillic acid is typical in wines (Xiao et al, 2015) and a constituent of vanilla flavor (Rao & Ravishankar, 2000). The vanillic acid has also been described in H. dulcis pseudofruits (Li et al, 2005).…”