Neuroprotective Effect of Defatted Sesame Seeds Extract againstin vitroandin vivoIschemic Neuronal Damage

Abstract

Sesame (Sesamum indicum L.) is an important oilseed crop that possesses a wide spectrum of pharmacological activities. Many studies have been conducted to investigate its health-promoting effects. Compared to other plant oils, sesame seed oil is highly stable to oxidation and has been demonstrated to have protective effects against ischemia-reperfusion injury in the rat brain. However; the effects of defatted sesame seeds extract (DSE) have not been studied yet. The purpose of this study was to evaluate the protective effect of DSE against ischemia models. For in vitro ischemia, oxygen-glucose deprivation followed by reoxygenation (OGD-R, 4 h OGD followed by 24 h reoxygenation) in HT22 cells was used to investigate the protective effects on cell death and the inhibitory effects on lipid peroxidation. For in vivo ischemia, the middle cerebral artery occlusion (MCAo, 2 h of MCAo followed by 22 h of reperfusion) rat model was used. Twenty-two h after occlusion the rats were assessed for neurobehavioral deficit and infarct volume. DSE (0.1-10 microg/mL) significantly reduced the cell death and inhibited lipid peroxidation induced by OGD-R. DSE (30, 100 and 300 mg/kg, p.o.) given twice at 0 h and 2 h after onset of ischemia reduced brain infarct volume dose-dependently and improved sensory-motor function. The therapeutic time window of DSE (300 mg/kg, p.o.) was 2 h after MCAo in rats. In conclusion, our results show that DSE may be effective in ischemia models by an antioxidative mechanism.

Sesamin ameliorates oxidative stress and mortality in kainic acid-induced status epilepticus by inhibition of MAPK and COX-2 activation

“…It is also consistent with the sesamin antioxidant effect that protects hypoxia-or H 2 O 2 -induced PC12 cell injury [22]. A study with defatted sesame seeds extract (30, 100 and 300 mg/kg) given twice orally at 0 h and 2 h after onset of ischemia shows the reduction of brain infarct volume dose-dependently and improves sensory-motor function [34]. Thus, suppression of KA-induced ROS and Ca 2+ release by sesamin was consistent with these notions.…”

Protective effect of a sesamin derivative, 3-bis (3-methoxybenzyl) butane-1, 4-diol on ischemic and hypoxic neuronal injury

“…Studies have shown that antioxidant compounds inhibit JNK MAPK activation in microglia represent potential anti-inflammatory effects and protect neurons damage [5,20,21]. In addition, antioxidant compounds inhibit JNK MAPK activation in neuron and cardiomyocyte cells represent potential protective effects from hypoxic damage [5,22-24]. Sesamin can regulate microglial activities by inhibition of the intracerebral hemorrhage-induced p44/42 MAPK pathway and protect neuronal cells by inhibition of hypoxia-induced ERK, JNK, p38 MAPK [5,25].…”

Defatted Sesame Seed Extract Reduces Brain Oedema by Regulating Aquaporin 4 Expression in Acute Phase of Transient Focal Cerebral Ischaemia in Rat

“…With regard to brain diseases, sesaminol glucoside, one of the components of the extract, was reported to have protective effects in Alzheimer disease models (Um et al , ). In a previous study, the neuroprotective effects of defatted sesame extract (DSE) and the mechanisms of action in ischaemia models were also reported (Jamarkattel‐Pandit et al , ).…”