Neuroprotective effect of Hydrocotyle sibthorpioides against monosodium glutamate-induced excitotoxicity

Abstract

We aimed to evaluate the neuroprotective effect of H. sibthorpioides against monosodiumglutamate (MSG) induced excitoneurotoxicity in rats. Animals were randomly divided into 11 groups (n=8) and subjected to high-doses of MSG (2g/kg body weight) along with the test-dose (1 week). The test-chemicals were H. sibthorpioides extracts of petroleum ether, chloroform, methanol, and water. Dizocilpine-hydrogen-maleate was used as a standard.Cognitive property was assessed using Morris-water-maze and elevated-plus-maze. On the 9 th -day, the animals were sacrificed and evaluated for their biochemical parameters. We found chloroform and methanolic extracts significantly enhanced the cognitive behavior of rats vs. control. Biochemical analysis suggested that there was a high level of antioxidants and lower levels of glutamate and proinflammatory cytokines in the cortex and hippocampus.We concluded that chloroform and methanolic extracts of H. sibthorpioides enhanced the level of antioxidants, decreased proinflammatory-cytokines and glutamate in brain and thus prevented the monosodium-glutamate-induced-excite-neurotoxicity.

Sub-Acute Toxicity Study of Pegagan Embun (Hydrocotyle Sibthorpioides Lam.) Extract on the SGPT and Sgot Level of Wistar White Male Rats

“…According to biochemical research, the cortex and hippocampus had higher amounts of antioxidants and lower levels of glutamate and proinflammatory cytokines. The researcher came to the conclusion that H. sibthorpioides extracts in chloroform and methanol increased the amount of antioxidants in the brain, lowered pro-inflammatory cytokines and glutamate, and so avoided the monosodiumglutamate-induced-excite-neurotoxicity [28]. Quanfang et al also examined the effect of Hydrocotyle sibthorpioides-derived genistein on Chronic Alcohol-Induced Hepatic Fibrosis.…”

Нейрохімічний Механізм Формування Поведінкових Реакцій За Умов Емоційного Навантаженняв Ранньому Онтогенезі

“…Не менш відома концепція участі глутамату і ГАМК у формуванні деперсивноподібних станів у тварин. Як нестача глутамату, так і надлишок вміcту ГАМК в мозку можуть призводити до розвитку депресії і психозів [16,20]. Водночас, накопичення глутамату, особливо в гіпокампі, який є ведучою структурою ЦНС в організації просторової пам'яті у щурів, супроводжувалось нейротоксичним ефектом та, як наслідок, погіршенням когнітивної функції.…”