Neuroprotective Effect of Lutein in Scopolamine-Induced Alzheimer’s Disease in Mice and Zebrafish

Patel, Chirag; Patel, Priyanshi; Dipta, Sarkar; Bulsara, Jeshica; Soni, Arun; Isapure, Kiran; Acharya, Sanjeev

doi:10.1007/s43450-021-00202-0

Cited by 7 publications

(4 citation statements)

References 31 publications

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“…The selected scopolamine-induced zebrafish model for Alzheimer’s disease is established and widely used to investigate the potential effect of natural, synthetic, and semisynthetic compounds. Furthermore, the T-maze, open field test (OFT), and light–dark chamber tests (LDT) have been extensively documented in zebrafish AD models for evaluating learning and memory impairments, motor abnormalities, and anxiety-like behaviors as key hallmarks of the disease. − …”

Section: Biological Resultsmentioning

confidence: 99%

In Vitro and In Vivo Investigations of Chromone Derivatives as Potential Multitarget-Directed Ligands: Cognitive Amelioration Utilizing a Scopolamine-Induced Zebrafish Model

Kumar,

Jangid,

Kumar

et al. 2024

ACS Chem. Neurosci.

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Alzheimer’s disease is a complex neurological disorder linked with multiple pathological hallmarks. The interrelation of therapeutic targets assists in the enhancement of cognitive decline through interference with overall neuronal transmission. We have synthesized and screened various chromone derivatives as potential multitarget-directed ligands for the effective treatment of Alzheimer’s disease. The synthesized compounds exhibited multipotent activity against AChE, BuChE, MAO-B, and amyloid β aggregation. Three potent compounds, i.e., VN-3, VN-14, and VN-19 were identified that displayed remarkable activities against different targets. These compounds displayed IC50 values of 80 nM, 2.52 μM, and 140 nM against the AChE enzyme, respectively, and IC50 values of 2.07 μM, 70 nM, and 450 nM against the MAO-B isoform, respectively. VN-3 displayed potent activity against self-induced Aβ1–42 aggregation with inhibition of 58.3%. In the ROS inhibition studies, the most potent compounds reduced the intracellular ROS levels up to 80% in SH-SY5Y cells at 25 μM concentration. The compounds were found to be neuroprotective and noncytotoxic even at a concentration of 25 μM against SH-SY5Y cells. In silico studies showed that the compounds were nicely accommodated in the active sites of the receptors along with thermodynamically stable orientations. Compound VN-19 exhibited a balanced multitargeting profile against AChE, BuChE, MAO-B, and Aβ1–42 enzymes and was further evaluated for in vivo activities on the scopolamine-induced zebrafish model. VN-19 was found to ameliorate the cognitive decline in zebrafish brains by protecting them against scopolamine-induced neurodegeneration. Thus, VN-3, VN-14, and VN-19 were identified as potent multitarget-directed ligands with a balanced activity profile against different targets and can be developed as therapeutics for AD.

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Section: Biological Resultsmentioning

confidence: 99%

In Vitro and In Vivo Investigations of Chromone Derivatives as Potential Multitarget-Directed Ligands: Cognitive Amelioration Utilizing a Scopolamine-Induced Zebrafish Model

Kumar,

Jangid,

Kumar

et al. 2024

ACS Chem. Neurosci.

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“…Another study has shown that Aβ (1–42)-treated mice had less interest in entering different arms of the Y-maze, which was later neutralized with the administration of quinovic acid and, in turn, enhanced the memory of mice ( Saeed et al, 2020 ). The neurotherapeutic efficacy of lutein on scopolamine-induced memory loss in mice and zebrafish has also been documented ( Patel et al, 2021 ).…”

Section: Discussionmentioning

confidence: 99%

Ranuncoside’s attenuation of scopolamine-induced memory impairment in mice via Nrf2 and NF-ĸB signaling

Sara Salahuddin,

Attaullah,

Ali Shah

et al. 2023

Saudi Pharmaceutical Journal

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“…The median lethal dose (LD 50 ) of SRRE was determined as per the organization for economic cooperation and development (OECD)-425 (2008) guidelines ( 15 ). For the acute toxicity study of the extracts, one rat received SRRE (175 mg/kg) as a single oral dose.…”

Section: Methodsmentioning

confidence: 99%

Effect of Shorea robusta resin extract in 3-nitropropionic acid-induced Huntington’s disease symptoms in Sprague-Dawley rats

Patel¹,

Boddu²,

Thakur³

et al. 2023

Research in Pharmaceutical Sciences

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Background and purpose: Huntington’s disease (HD) is a neurodegenerative disease characterized by neuronal death in the striatum. Asiatic acid is an active component of Shorea robusta (Dipterocarpaceae) plants with neuroprotective activity and is considered an acceptable therapeutic candidate for different neurodegenerative diseases. In the present study, the beneficial pharmacological action of Shorea robusta resin extract (SRRE) was assessed in 3-nitropropionic acid (3-NP)-induced HD in rats. Experimental approach: The neuroprotective effect of SRRE (285.7 and 666.7 mg/kg, p.o., 14 days) was studied in 3-NP (10 mg/kg)-induced rats by measuring body weight, behavioral parameters including neurological scoring, motor coordination, spatial memory, and depression-like behavior, neuro-biochemical parameters (gamma-aminobutyric acid and acetylcholinesterase), and oxidative stress parameter in the brain. Histopathology of the rat’s brain was also studied. Findings/Results: SRRE treatment (285.7 mg/kg and 666.7 mg/kg) substantially restored body weight, motor coordination, and mitochondrial enzyme complex I function and improved memory impairment as compared to 3-NP-treated rats. Furthermore, SRRE treatment significantly restored the antioxidant enzyme activity in brain tissue and ameliorated the histopathological changes induced by 3-NP. Conclusion and implications: The neuroprotective effect of SRRE on 3-NP-induced HD in rats was mediated by a reduction in oxidative stress which may favor the usefulness of Shorea robusta in HD.

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Neuroprotective Effect of Lutein in Scopolamine-Induced Alzheimer’s Disease in Mice and Zebrafish

Cited by 7 publications

References 31 publications

In Vitro and In Vivo Investigations of Chromone Derivatives as Potential Multitarget-Directed Ligands: Cognitive Amelioration Utilizing a Scopolamine-Induced Zebrafish Model

In Vitro and In Vivo Investigations of Chromone Derivatives as Potential Multitarget-Directed Ligands: Cognitive Amelioration Utilizing a Scopolamine-Induced Zebrafish Model

Ranuncoside’s attenuation of scopolamine-induced memory impairment in mice via Nrf2 and NF-ĸB signaling

Effect of Shorea robusta resin extract in 3-nitropropionic acid-induced Huntington’s disease symptoms in Sprague-Dawley rats

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