“…Moreover, to discriminate the species of herbal medicine, Holotrichia was used in this study. We amplified and sequenced COI DNA barcode regions, an universal DNA barcode used for identifying animal species, from randomly selected five larvae and compared the sequence identity with other COI sequences registered in the GenBank using BLAST (Basic Local Alignment Search Tool) analysis (Che et al., 2012 ; Lee et al., 2021 ). To confirm the sequence identity and species of our samples, furthermore, comparative sequence analysis was carried out between the COI sequences of five samples and other closely related insect species registered in the GenBank including Protaetia brevitarsis (KC775706), P. affinis (KM286290), P. speciosissima (KM286124 and KJ908751), P. lunubris (KM286218, KU908751, and KU916955), P. marmorata (KJ964464), P. cuprea (DQ295301), P. aurichalcea (KM033437), and P. morio (KY827323).…”