Neuroprotective Effect of Resveratrol on Valproic Acid Induced Oxidative Stress Autism in Swiss Albino Mice

Abstract

The present research work is aimed to investigate the anti-oxidant/neuroprotective role of Resveratrol in reversing the valproic acid induced autism in postnatal swiss albino mice. Separate 13 day old/ Post natal day (PND) 13 swiss albino mice of either sex into 5 groups, each group consists of six mice of either sex, groups namely Group I - Control, Group II - Resveratrol, Group III - Negative control, Group IV and V - Resveratrol treatment groups. On PND 14 administer single dose of Valproic acid (VPA) or Sodium Valproate (400 mg/kg, subcutaneously) to III, IV and V groups to induce autism. Treatment is given in two doses 10 mg/kg, intraperitoneally (i.p) as Low dose and 40 mg/kg, i.p as High dose from the 13th day to the end of study. Assessment of autism is done by different behavioral screening methods during PND 14 to 40. Treatment with resveratrol significantly decline the autism symptoms compared with negative control. At the end of study on PND 41 all the animals were sacrificed to assess the biochemical estimations like Anticholinesterase enzyme, Total Protein, antioxidant enzyme (Catalase, Superoxide and Glutathione) activity and cerebellar histopathological examination. Treatment with Resveratrol has shown a significant beneficial difference on behavioral alterations, oxidative markers, neurotransmitters, and restoration of the altered purkinje cells of autism. This research work we conclude that resvertrol have a potent anti-oxidant, neuroprotective, anxiolytic, learning and memory enhancing agent against valproic acid induced autism.

Medroxyprogesterone acetate improves propionic acid-induced autism rat model and magnetic resonance spectroscopic correlation

“…Many neuroprotective agents have been reported in the autism model of rats and mice. These neuroprotective agents improved the social behavior, decreased the level of inflammatory cytokines, improved the morphological abnormalities of Purkinje cells, and decreased the percentage of immunopositivity of glial fibrillar acidic protein in hippocampus and cerebellum of autistic rats and mice (Al-Gholam and Ameen, 2020;Bakshi et al, 2018;Tiwari et al, 2021;Al-Amin et al, 2015;Arafat and Shabaan, 2019;Sharma et al, 2019). The improved results of medroxyprogesterone in the cerebellum and hippocampus of rats are quite similar to the results of these studies of neuroprotective agents.…”

“…In a study of valproic acid-induced autism in mice, an increased level of inflammatory cytokines, higher acti- vity of glial cells, and morphological abnormalities in Purkinje cells were described (Al-Gholam and Ameen, 2020). Abnormal behavioral activities, oxidative stress, and abnormalities in Purkinje cells have been reported in another study of autism in mice (Bakshi et al, 2018). Higher activity of inflammatory cytokines (TNF-α, interleukins), abnormal social behavior, higher level of apoptotic markers, and lower level of anti-apoptotic markers and morphological abnormalities were found in the propionic acid-induced autism model of rats (Tiwari et al, 2021).…”