Neuroprotective effect of sulfated polysaccharide isolated from sea cucumber Stichopus japonicus on 6-OHDA-induced death in SH-SY5Y through inhibition of MAPK and NF-κB and activation of PI3K/Akt signaling pathways

Cui, Chao; Cui, Ningshan; Wang, Peng; Song, Shuliang; Liang, Hao; Ji, Aiguo

doi:10.1016/j.bbrc.2016.01.035

Cited by 36 publications

(25 citation statements)

References 20 publications

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“…Cui et al [38] studied the protective effect of sulfated polysaccharide (such as SJP) against 6-OHDA-induced SH-SY5Y cell death and found that the Δ Ψ m of SJP pretreatment group (79%) was higher than 6-OHDA damaged group (56%), suggesting that SJP had certain protective effect on 6-OHDA-induced SH-SY5Y cell damage. Li et al [39] examined the endothelial cell death mode caused by high glucose-induced ROS and suggested that Ganoderma atrum polysaccharide (PSG-1) had protective effect on the Δ Ψ m. The Δ Ψ m (36.26%) of high glucose-treated group was obviously lower than the cell of the control group (80.44%), but the Δ Ψ m increased (60.03%) after the cells were pretreated by PSG-1.…”

Section: Resultsmentioning

confidence: 99%

Effect of Content of Sulfate Groups in Seaweed Polysaccharides on Antioxidant Activity and Repair Effect of Subcellular Organelles in Injured HK‐2 Cells

Sun

et al. 2017

Oxidative Medicine and Cellular Longevity

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This study aims to investigate the repair effect of subcellular structure injuries of the HK-2 cells of four degraded seaweed polysaccharides (DSPs), namely, the degraded Porphyra yezoensis, Gracilaria lemaneiformis, Sargassum fusiform, and Undaria pinnatifida polysaccharides. The four DSPs have similar molecular weight, but with different content of sulfate groups (i.e., 17.9%, 13.3%, 8.2%, and 5.5%, resp.). The damaged model was established using 2.8 mmol/L oxalate to injure HK-2 cells, and 60 μg/mL of various DSPs was used to repair the damaged cells. With the increase of sulfate group content in DSPs, the scavenging activity of radicals and their reducing power were all improved. Four kinds of DSPs have repair effect on the subcellular organelles of damaged HK-2 cells. After being repaired by DSPs, the release amount of lactate dehydrogenase was decreased, the integrity of cell membrane and lysosome increased, the Δψm increased, the cell of G1 phase arrest was inhibited, the proportion of S phase increased, and cell apoptotic and necrosis rates were significantly reduced. The greater the content of sulfate group is, the stronger is the repair ability of the polysaccharide. These DSPs, particularly the polysaccharide with higher sulfate group content, may be a potential drug for the prevention and cure of kidney stones.

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Section: Resultsmentioning

confidence: 99%

Effect of Content of Sulfate Groups in Seaweed Polysaccharides on Antioxidant Activity and Repair Effect of Subcellular Organelles in Injured HK‐2 Cells

Sun

et al. 2017

Oxidative Medicine and Cellular Longevity

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“…NF‐κB regulates transcription of genes involved in immune responses, inflammation, cell differentiation, proliferation, and apoptosis . NF‐κB, which has been reported to increase in dopaminergic neurons of PD, plays a prominent role in 6‐OHDA‐induced SH‐SY5Y cell death . Inflammation is a tissue response to injury, ischemia, autoimmunity, toxic metabolites, or infectious agents.…”

Section: Introductionmentioning

confidence: 99%

NF‐κB/p53‐activated inflammatory response involves in diquat‐induced mitochondrial dysfunction and apoptosis

Choi

Park

Koh³

2018

Environmental Toxicology

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Inflammation generated by environmental toxicants including pesticides could be one of the factors underlying neuronal cell damage in neurodegenerative diseases. In this study, we investigated the mechanisms by which inflammatory responses contribute to apoptosis in PC12 cells treated with diquat. We found that diquat induced apoptosis, as demonstrated by the activation of caspases and nuclear condensation, inhibition of mitochondrial complex I activity, and decreased ATP level in PC12 cells. Diquat also reduced the dopamine level, indicating that cell death induced by diquat is due to cytotoxicity of dopaminergic neuronal components in these cells. Exposure of PC12 cells to diquat led to the production of reactive oxygen species (ROS), and the antioxidant N-acetyl-cystein attenuated the cytotoxicity of caspase-3 pathways. These results demonstrate that diquat-induced apoptosis is involved in mitochondrial dysfunction through production of ROS. Furthermore, diquat increased expression of cyclooxygenase-2 (COX-2) and tumor necrosis factor-α (TNF-α) via inflammatory stimulation. Diquat induced nuclear accumulation of NF-κB and p53 proteins. Importantly, an inhibitor of NF-κB nuclear translocation blocked the increase of p53. Both NF-κB and p53 inhibitors also blocked the diquat-induced inflammatory response. Pretreatment of cells with meloxicam, a COX-2 inhibitor, also blocked apoptosis and mitochondrial dysfunction. These results represent a unique molecular characterization of diquat-induced cytotoxicity in PC12 cells. Our results demonstrate that diquat induces cell damage in part through inflammatory responses via NF-κB-mediated p53 signaling. This suggests the potential to generate mitochondrial damage via inflammatory responses and inflammatory stimulation-related neurodegenerative disease.

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“…Nevertheless, other stress-response signalling pathways may also be involved. For example, a sulfated polysaccharide isolated from sea cucumber Stichopus japonicus prevents 6-OHDA cytotoxicity through activation of PI3K/Akt signalling pathway in human neuroblastoma SH-SY5Y cells [45]. However, the exact signalling mechanisms underlying the neuroprotective effect of astragalan need further investigation.…”

Section: Resultsmentioning

confidence: 99%

Astragalus Polysaccharide Suppresses 6‐Hydroxydopamine‐Induced Neurotoxicity in Caenorhabditis elegans

Shi

Ding

et al. 2016

Oxidative Medicine and Cellular Longevity

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Astragalus membranaceus is a medicinal plant traditionally used in China for a variety of conditions, including inflammatory and neural diseases. Astragalus polysaccharides are shown to reduce the adverse effect of levodopa which is used to treat Parkinson's disease (PD). However, the neuroprotective effect of Astragalus polysaccharides per se in PD is lacking. Using Caenorhabditis elegans models, we investigated the protective effect of astragalan, an acidic polysaccharide isolated from A. membranaceus, against the neurotoxicity of 6-hydroxydopamine (6-OHDA), a neurotoxin that can induce parkinsonism. We show that 6-OHDA is able to degenerate dopaminergic neurons and lead to the deficiency of food-sensing behavior and a shorter lifespan in C. elegans. Interestingly, these degenerative symptoms can be attenuated by astragalan treatment. Astragalan is also shown to alleviate oxidative stress through reducing reactive oxygen species level and malondialdehyde content and increasing superoxide dismutase and glutathione peroxidase activities and reduce the expression of proapoptotic gene egl-1 in 6-OHDA-intoxicated nematodes. Further studies reveal that astragalan is capable of elevating the decreased acetylcholinesterase activity induced by 6-OHDA. Together, our results demonstrate that the protective effect of astragalan against 6-OHDA neurotoxicity is likely due to the alleviation of oxidative stress and regulation of apoptosis pathway and cholinergic system and thus provide an important insight into the therapeutic potential of Astragalus polysaccharide in neurodegeneration.

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Neuroprotective effect of sulfated polysaccharide isolated from sea cucumber Stichopus japonicus on 6-OHDA-induced death in SH-SY5Y through inhibition of MAPK and NF-κB and activation of PI3K/Akt signaling pathways

Cited by 36 publications

References 20 publications

Effect of Content of Sulfate Groups in Seaweed Polysaccharides on Antioxidant Activity and Repair Effect of Subcellular Organelles in Injured HK‐2 Cells

Effect of Content of Sulfate Groups in Seaweed Polysaccharides on Antioxidant Activity and Repair Effect of Subcellular Organelles in Injured HK‐2 Cells

NF‐κB/p53‐activated inflammatory response involves in diquat‐induced mitochondrial dysfunction and apoptosis

Astragalus Polysaccharide Suppresses 6‐Hydroxydopamine‐Induced Neurotoxicity in Caenorhabditis elegans

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