Neuroprotective effect of total glycosides from paeonies against neurotoxicity induced by strychnos alkaloids related to recovering the levels of neurotransmitters and neuroendocrine hormones in rat serum and brain

Sun, Linjia; Chen, Yu; Hou, Chenzhi; Sun, Xiaoyang; Wang, Zhipeng; Li, Shujuan; Lv, Mingming; Chen, Xiaohui

doi:10.1039/c8ra05384g

Cited by 11 publications

(14 citation statements)

References 58 publications

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“…Total paeony glycosides (TPGs) are the main active constituents in CS and total ligustici phenolic acids (TLPAs) are the major active components in CX. TPGs were reported to have a good effect on both cerebral ischemia and cardiac ischemia 16,17 . Table 5.…”

Section: Discussionmentioning

confidence: 99%

The effect and mechanism of combination of total paeony glycosides and total ligustici phenolic acids against focal cerebral ischemia

Feng

Song

et al. 2020

Sci Rep

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The root of Paeonia lactiflora Pall. (Chishao, CS) and Ligusticum chuanxiong Hort. (Chuanxiong, CX) were widely used as a drug pair in Chinese Medicine, and the combination of CS and CX showed a more significant inhibition on neuronal apoptosis in our previous study. In the present study, total paeony glycosides (TPGs) from CS and total ligustici phenolic acids (TLPAs) from CX were combined to evaluate the synergistic effects against focal cerebral ischemia both in vitro and in vivo. The combination of TPGs and TLPAs at 7:3 had the best anti-oxidative stress and anti-inflammatory effect on OGD-induced HUVEC. Additionally, the infarction area proportion and neuron apoptosis of rats by TPGs:TLPAs (7:3) was significantly lower than their alone in MCAO rats. Moreover, TPGs: TLPAs of 7:3 showed a more significant effect on decreasing the expression of MMP-2 and MMP-9, and increasing the protein expression or mRNA level of TIMP-1 than other combinations. The optimal ratio of TPGs and TLPAs at 7:3 could bring more remarkable protective effects against focal cerebral ischemia in MCAO rats by alleviating oxidative stress, inflammatory and neuronal apoptosis to protect the blood-brain barrier. Overall, the present study provided benefical evidence for clinical application of CS and CX as a "drug pair". As the third highest worldwide cause of mortality 1 , stroke has been treated using antithrombotic therapy 2 , anticoagulant therapy 3,4 , neuroprotective and vascular dilated therapy. Numerous studies have demonstrated that the severity of neuronal apoptosis was associated with neurofunction, indicating a crucial role in the prognosis of ischemic stroke sufferer 5. Early anti-inflammatory and anti-oxidative stress may promote apoptosis of nerve cells. Thus attenuating these potential damage of nerve cells in ischemic area will be an effective strategy in clinical application. Chinese medicine (CM) has been used to treat stroke for a long time. Many classical prescriptions for treating cerebral diseases, such as Xiao-xu-ming decoction 6 are still being commonly used in modern clinial both in China and in other countries 7,8. Paeonia lactiflora Pall. (Chinese name: Chishao, CS) has been used worldwide for centuries and reported to exhibit various pharmacological bioactivities including anticoagulation, cognition enhancement, immunoregulatory 9 and anticance 10 , antioxidative 11 , and antidiabetic properties 12. The ischemic stroke protection of Paeoniae Radix may be related to its anti-oxidative stress and improvement of brain microcirculation. Ligusticum chuanxiong Hort. (Chinese name: Chuanxiong, CX) which belongs to the Umbelliferae family, is a valuable medicinal herb used worldwide and has been used for the treatment of atherosclerosis, hypertension, angina pectoris, and anti-inflammatory via acilitating blood circulation and dispersing blood stasis 13. Our previous study revealed the combination of CS and CX exerted more remarkable protective effects than alone by attenuating ER-stress-dependent apoptotic signaling and...

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Section: Discussionmentioning

confidence: 99%

The effect and mechanism of combination of total paeony glycosides and total ligustici phenolic acids against focal cerebral ischemia

Feng

Song

et al. 2020

Sci Rep

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“…In rat models, PF administration attenuated cognitive deficits induced by scopolamine [51]. In addition, supplementation of PF significantly alleviated the neurotoxic effect through regulation of neurotransmitters and hormones in rat serum and brain [52]. According to previous studies, PF was detectable immediately in the cortex and hippocampus of rats after intravenous administration of Paeoniae Radix extract containing PF [53,54].…”

Section: Discussionmentioning

confidence: 87%

Protective role of paeoniflorin from hydrogen peroxide-mediated oxidative damage in C6 glial cells

Lee

Nam

Kim

et al. 2020

JABC

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Oxidative stress is one of the pathogenic mechanisms of various neurodegenerative diseases, such as Alzheimer's disease. Neuroglia, the most abundant cells in the brain, is thought to play an important role in the antioxidant defense system and neuronal metabolic support against neurotoxicity and oxidative stress. We investigated the protective effect of paeoniflorin (PF) against oxidative stress in C6 glial cells. Exposure of C6 glial cells to hydrogen peroxide (H 2 O 2 , 500 μM) significantly decreased cell viability and increased amounts of lactate dehydrogenase (LDH) release, indicating H 2 O 2-induced cellular damage. However, treatment with PF significantly attenuated H 2 O 2-induced cell death as shown by increased cell survival and decreased LDH release. The H 2 O 2stimulated reactive oxygen species production was also suppressed, and it may be associated with improvement of superoxide dismutase activity by treatment with PF. In addition, an increase in ratio of Bcl-2/Bax protein expression was observed after treatment with PF. In particular, the downstream of the apoptotic signaling pathway was inhibited in the presence of PF, mostly by reduction of cleaved-poly ADP ribose polymerase, cleaved caspase-3, and-9 protein expression. Furthermore, H 2 O 2-induced phosphorylation of c-Jun N-terminal kinase and extracellular signal-regulated kinase 1/2 was attenuated by treatment with PF. Taken together, neuroprotective effect of PF against oxidative stress probably result from the regulation of apoptotic pathway in C6 glial cells. In conclusion, our findings suggest that PF may be a potent therapeutic agent for neurodegenerative disorders.

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“…Another study detected seven neurotransmitters with the help of microdialysis coupled with hydrophilic interaction chromatography–tandem mass spectrometry (MD-HILIC–MS/MS) [ 57 ]. In the other studies, eight neurotransmitters were studied using LC-MS [ 58 , 59 ]. Only one study reported quantification of 10 neurotransmitters and their metabolites [ 60 ].…”

Section: Introductionmentioning

confidence: 99%

“…Analytical method by Linjia Sun et al[59]:i. Chromatography technique: liquid chromatographytandem mass spectrometry (LC-MS/MS) method ii.…”

mentioning

confidence: 99%

Application of Three Ecological Assessment Tools in Examining Chromatographic Methods for the Green Analysis of a Mixture of Dopamine, Serotonin, Glutamate and GABA: A Comparative Study

et al. 2021

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The assessment of greenness of analytical protocols is of great importance now to preserve the environment. Some studies have analyzed either only the neurotransmitters, dopamine, serotonin, glutamate, and gamma-aminobutyric acid (GABA), together or with other neurotransmitters and biomarkers. However, these methods have not been investigated for their greenness and were not compared with each other to find the optimum one. Therefore, this study aims to compare seven published chromatographic methods that analyzed the four neurotransmitters and their mixtures using the National Environmental Method Index, Analytical Eco-Scale Assessment (ESA), and Green Analytical Procedure Index (GAPI). As these methods cover both qualitative and quantitative aspects, they offer better transparency. Overall, GAPI showed maximum greenness throughout the analysis. Method 6 was proven to be the method of choice for analyzing the mixture, owing to its greenness, according to NEMI, ESA, and GAPI. Additionally, method 6 has a wide scope of application (13 components can be analyzed), high sensitivity (low LOQ values), and fast analysis (low retention times, especially for glutamate and GABA).

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Neuroprotective effect of total glycosides from paeonies against neurotoxicity induced by strychnos alkaloids related to recovering the levels of neurotransmitters and neuroendocrine hormones in rat serum and brain

Abstract: Eight neurotransmitters and four neuroendocrine hormones in rat serum and brain were quantified to investigate the neuroprotective effect of total glycosides from paeony against neurotoxicity induced by strychnos alkaloids.

Cited by 11 publications

References 58 publications

The effect and mechanism of combination of total paeony glycosides and total ligustici phenolic acids against focal cerebral ischemia

The effect and mechanism of combination of total paeony glycosides and total ligustici phenolic acids against focal cerebral ischemia

Protective role of paeoniflorin from hydrogen peroxide-mediated oxidative damage in C6 glial cells

Application of Three Ecological Assessment Tools in Examining Chromatographic Methods for the Green Analysis of a Mixture of Dopamine, Serotonin, Glutamate and GABA: A Comparative Study

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