Neuroprotective Effects ofCentella asiaticaagainst Intracerebroventricular Colchicine-Induced Cognitive Impairment and Oxidative Stress

Abstract

Oxidative stress appears to be an early event involved in the pathogenesis of Alzheimer's disease. The present study was designed to investigate the neuroprotective effects of Centella asiatica against colchicine-induced memory impairment and oxidative damage in rats. Colchicine (15 μg/5 μL) was administered intracerebroventricularly in the lateral ventricle of male wistar rats. Morris water maze and plus-maze performance tests were used to assess memory performance tasks. Various biochemical parameters such as lipid peroxidation, nitrite, reduced glutathione, glutathione-S-transferase, superoxide dismutase, acetylcholinesterase were also assessed. ICV colchicine resulted marked memory impairment and oxidative damage. Chronic treatment with Centella asiatica extract (150 and 300 mg/kg, p.o.) for a period of 25 days, beginning 4 days prior to colchicine administration, significantly attenuated colchicine-induced memory impairment and oxidative damage. Besides, Centella asiatica significantly reversed colchicines administered increase in acetylcholinesterase activity. Thus, present study indicates protective effect of Centella asiatica against colchicine-induced cognitive impairment and associated oxidative damage.

Preparation and Evaluation of Phospholipid-Based Complex of Standardized Centella Extract (SCE) for the Enhanced Delivery of Phytoconstituents

“…The Bonferroni multiple comparison test revealed that aged animals treated with vehicle did not recognize the target quadrant, and therefore spent less time in that quadrant compared to the vehicletreated young animals (p<0.05). However, oral treatments of piracetam (p<0.01), or CN (p<0.05) significantly increased the time spent in the target quadrant compared to the vehicle-treated aged animals, thus confirming the potential effect of Centella extract on memory as reported in earlier studies (37,38). Centella extract is known to improve the morphology and arborization of hippocampal neuronal dendrites (52,53).…”

“…The doses used in the present study were reported effective in the animals (37,38). All animals received their respective treatments orally, each day, 1 h before the experiment.…”

“…Centella extract is known to improve the morphology and arborization of hippocampal neuronal dendrites (52,53). Moreover, its negative effect on reactive oxygen species and superoxide formation along with decrease in glutathione and activation of glutathione-Stransferase, have reportedly the key mechanisms involved in its nootropic action (37).…”

Ethanol Extracts of Centella asiatica (L.) Urb. Leaf Increase Superoxide Dismutase-2 (SOD-2) Expression on Rat Cerebellar Purkinje Cells After Chronic Stress

“…14 Phenolic compounds of C. asiatica (tocopherols, flavonoids, cinnamic acid, and phosphatidic) are an effective hydrogen donating radical scavengers by scavenging lipid alkoxyl andperoxyl radical. 14 Previous studies have reported thatphenolic compound of C. asiatica also exhibit another antioxidant activity, such ascatalase, glutathione peroxidase, vitamin C and vitamin E. 15,16 Although the mechanism were still unclear, we have added biochemical evidence that C. asiatica has a role as a neuroprotective agent in the brain, mainly in cerebellum.The consumption of C. asiatica was useful to protect the cells from oxidative damage, to destroy excess free radicals, and keep the oxidative stress state in balance. 2 These effects may be attributed to the anti oxidant compounds present in C. asiatica, that are known to be potent anti oxidants.…”

Effectiveness of Brahmi in Various Illnesses: Review Paper

“…It has been used as a memory enhancing, strength promoting, immune booster, anti-anxiety, antiepilepsy and anti-stress substance since ancient times [18,19]. Clinically, Centella asiatica has been effective in treatment of mentally retarded children and anxiety neurosis [20].…”