Neuroprotective effects of Liriope platyphylla extract against hydrogen peroxide-induced cytotoxicity in human neuroblastoma SH-SY5Y cells

Park, Hee Ra; Lee, Hee-Eun; Park, Hwayong; Jeon, Jong Wook; Cho, Won Kyung; Yeul, Jin

doi:10.1186/s12906-015-0679-3

Cited by 63 publications

(52 citation statements)

References 54 publications

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“…2). The data from the present study suggest that H 2 O 2 -induced p38MAPK activation facilitates neuron SH-SY5Y cell apoptosis, as previously considered (26). There is a limited data regarding the antioxidant role of SB203580; however, in the present study the inhibitor exhibited an antioxidant capacity by effectively reversing the H 2 O 2 -mediated increase of MDA (P<0.05; Fig.…”

Section: Resultssupporting

confidence: 82%

“…1D), as well as increased CAT and GPx activities significantly compared with the PC group ( Fig. 3C and E (26). In the present study, SH-SY5Y cells exposed to H 2 O 2 exhibited a 2.2-fold increase in p38MAPK phosphorylation at the sites of Thr180 and Tyr182 (P<0.05, Fig.…”

Section: Resultssupporting

confidence: 53%

“…Park et al (26) demonstrated that the phosphorylation level of p38MAPK is markedly increased in SH-SY5Y cells following H 2 O 2 stimulation, but those of extracellular signal regulated kinase and c-Jun N-terminal were not affected. Furthermore, increased p38MAPK phosphorylation is associated with an increase in the rate of SH-SY5Y cell apoptosis, which was eradicated following treatment with SB203580, a p38MAPK specific inhibitor (26). A similar outcome was observed in the present study and it was observed that OS-led neuronal apoptosis relies on p38MAPK phosphorylation at two sites (Thr180 and Tyr182), rather than at Thr322.…”

Section: Discussionmentioning

confidence: 99%

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α- and β-Naphthoflavone synergistically attenuate H2O2-induced neuron SH-SY5Y cell damage

Zhu

et al. 2017

Experimental and Therapeutic Medicine

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Abstract. Previous studies have demonstrated an association between neurological diseases and oxidative stress (OS).Naphthoflavone is a synthetic derivative of naturally occurring flavonoids that serves an important role in the treatment and prevention of OS-related diseases. The current study was designed to apply α-and β-Naphthoflavone individually and in combination to counteract the detrimental effects of OS on neurons in vitro. Neuronal SH-SY5Y cells were subjected to 20 µM H 2 O 2 , followed by exposure to 20 µM α-Naphthoflavone and/or 10 µM β-Naphthoflavone. Results indicated that α-and β-Naphthoflavone effectively antagonized the apoptosis-promoting effect of H 2 O 2 on neuronal SH-SY5Y cells, and that β-Naphthoflavone significantly (P<0.05) reversed H 2 O 2 -inhibited cell viability. Notably, co-treatment of α-and β-Naphthoflavone reversed the H 2 O 2 -induced apoptosis rate elevation and cell viability reduction. Further analysis demonstrated that H 2 O 2 inhibited the activities of antioxidant enzymes including catalase, superoxide dismutase and glutathione peroxidase, but this was reversed by the co-treatment with α-and β-Naphthoflavone and selectively enhanced by the treatment with α-or β-Naphthoflavone. H 2 O 2 -stimulated p38 mitogen-activated protein kinase activation was repressed following treatment with α-and/or β-Naphthoflavone, along with a decreased expression of the apoptosis-related factors and inhibited caspase-3 activation. In conclusion, co-treatment with α-and β-Naphthoflavone minimized H 2 O 2 -led neuron damage compared with treatment with α-or β-Naphthoflavone, suggesting a synergetic effect between α-and β-Naphthoflavone. This indicates that utilizing α-and β-Naphthoflavone together in the clinical setting may provide a novel therapeutic for neurological disease.

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Section: Resultssupporting

confidence: 82%

Section: Resultssupporting

confidence: 53%

Section: Discussionmentioning

confidence: 99%

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α- and β-Naphthoflavone synergistically attenuate H2O2-induced neuron SH-SY5Y cell damage

Zhu

et al. 2017

Experimental and Therapeutic Medicine

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“…Ethanol extract of LR attenuated the H 2 O 2 -induced increases of intracellular oxidative stress and mitochondrial dysfunction. It also blocked the PARP and caspase-3 cleavage by modulating p38 activation in SH-SY5Y cells [55]. The 70 % ethanol extract of LR treatments significantly increased the latency time with increased brain-derived neurotrophic factor (BDNF) immunopositive cells and increased NGF [56].…”

Section: Neuroprotective Activitymentioning

confidence: 97%

Phytochemical and pharmacological overview on <i>Liriopes radix</i>

Mahesh¹,

Kim²

2016

Trop. J. Pharm Res

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Liriopogons (Liriope and Opiopogon) species are used as the main medicinal ingredient in traditional medicine in several Asian countries. The roots or tubers of Liriope plants (Liriopes radix; LR) have traditionally been used for hundreds of years to treat cough, insomnia, constipation, asthma, and inflammation. The present review discusses extensively the available knowledge on its phytochemical and pharmacological activities in vitro and in vivo. The review does not include other parts of these plants. Literature evidence has been analyzed to identify responsible phytochemicals and their wide range of pharmacological activities. Further studies are needed for the isolation of purified compounds in order to understand their mechanisms of action and for clinical application.

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“…Nevertheless, H2O2 is intrinsically stable, but at higher sustained levels it generates highly reactive OH ·-Several animal studies show convincing protective effects of antioxidants against ischemia-induced damage including the cell/brain permeanttocoferol analogue, Trolox [11] and the SODmimetic (Mn(III)tetrakis(1-methyl-4-pyridyl)porphyrinpentachloride) reduced lipid peroxidation and DNA fragmentation [12]. radicals via the Fenton reaction which oxidizes almost any cell constituent leading to cellular dysfunction [9,10].…”

Section: Introductionmentioning

confidence: 99%

Neuroprotective Effect of Artocarpus Lakoocha Extract and Oxyresveratrol Against Hydrogen Peroxide-Induced Toxicity in Sh-Sy5y Cells

Hasriadi

Wongon

Lapphanichayakool

et al. 2017

Int J Pharm Pharm Sci

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Objective: Artocarpuslakoocha Roxb. Is a traditional medical plant native to Southeast Asia and used as a dried aqueous extract so-called puaghaad. Its role (and its major ingredient, oxyresveratrol) as an antioxidant neuroprotectant were explored.Methods: Differentiated SH-SY5Y neuroblastoma cells in 96-well plates were challenged with 200 µM H2O2 for 4h and either Trolox (100 µM), oxyresveratrol (5-100 µM), or puaghaad (1.2-25 µg/ml) applied 2h before H2O2 or for 20 h after H2O2Results: Continuous presence of both H washout. Cell viability, mitochondrial function, intracellular ROS, and lipid peroxidation were assessed.2O 2 and antioxidant reduced mitochondrial function by ~50% but only by 30% with antioxidant. Sustained 24 h H2O2 showed no recoveries with antioxidants. Cell viability was modestly restored when antioxidants accompanied H2O2 for 4 h and both washed for another 20 h, but little recovery of mitochondrial function even though antioxidants removed ROS and prevent lipid peroxidation. Antioxidants added for 20 h after H2O2 Conclusion:These results show that mitochondrial protection was illusive, yet both tested compounds, puaghaad and oxyresveratrol, improved cell viability and especially ROS levels and lipid peroxidation. The potency oxyresveratrol on theredox-sensitive expression of antioxidant enzymes and its pharmacokinetics suggests that oral puaghaad could provide effective protection in transient neurodegenerative disease. marginally improve mitochondria and modestly restore cell viability, but lipid peroxidation was completely reversed.

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Neuroprotective effects of Liriope platyphylla extract against hydrogen peroxide-induced cytotoxicity in human neuroblastoma SH-SY5Y cells

Cited by 63 publications

References 54 publications

α- and β-Naphthoflavone synergistically attenuate H2O2-induced neuron SH-SY5Y cell damage

α- and β-Naphthoflavone synergistically attenuate H2O2-induced neuron SH-SY5Y cell damage

Phytochemical and pharmacological overview on <i>Liriopes radix</i>

Neuroprotective Effect of Artocarpus Lakoocha Extract and Oxyresveratrol Against Hydrogen Peroxide-Induced Toxicity in Sh-Sy5y Cells

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