Neuroprotective effects of lycopene in spinal cord injury in rats via antioxidative and anti-apoptotic pathway

Hu, Wei; Wang, Hongbo; Liu, Zhongfan; Liu, Yanlu; Wang, Rong; Luo, Xiao; Huang, Yifei

doi:10.1016/j.neulet.2017.02.004

Cited by 45 publications

(27 citation statements)

References 31 publications

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“…Other studies also demonstrated that SCI can induce cell apoptosis [39,40]. In addition, lycopene treatment was reported to protect against SCI by decreasing neuron apoptosis [38]. In our study, the SCI group showed increased neuron apoptosis, decreased Bcl2 expression, and increased expression of Bax and cleaved caspase3.…”

Section: Discussionsupporting

confidence: 69%

“…In the present study, BML-111 improved SCI by effectively reversing the increased levels of TAS and TOS in serum. Hu et al [38] found that SCI-induced oxidative injury can lead to neuron apoptosis, which subsequently induced changes in expression of apoptosis-related proteins. Other studies also demonstrated that SCI can induce cell apoptosis [39,40].…”

Section: Discussionmentioning

confidence: 99%

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The Lipoxin A4 Receptor Agonist BML-111 Alleviates Inflammatory Injury and Oxidative Stress in Spinal Cord Injury

Liu

Peng

2020

Med Sci Monit

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Departmental sources Background: Spinal cord injury (SCI) has a high incidence and causes serious harm. Lipoxin A4 (LXA4) receptor agonist BML-111 was reported to regulate inflammation and oxidative stress. The goal of this study was to assess whether BML-111 could protect against SCI by suppressing inflammation and oxidative stress. Material/Methods: We developed a rat SCI model, then BML-111 was intraperitoneally injected into SCI rats to observe the BML-111 function. The pathological changes of SCI were observed with hematoxylin and eosin (HE) staining. Motor function of rats were assessed by the modified Tarlov's scale. ELISA was used to assess the changes in levels of TNF-a, IL-1b, and IL-6. Western blot analysis was performed to assess the expressions of TNF-a, IL-1b, IL-6, Bcl2, Bax, and cleaved caspase3 in spinal cord tissue. TOS and TAS in rat serum were detected by xylenol orange method and ABTS method, respectively. The apoptotic cells in spinal cord tissue were observed with TUNEL assay. Results: The results indicated that BML-111 effectively improved the SCI and motor function of rats. BML-111 treatment decreased the levels of TNF-a, IL-1b, and IL-6 in serum and spinal cord tissue, as well as decreasing the levels of TOS and TAS and cell apoptosis. Conclusions: BML-111 alleviated inflammation and oxidative stress in SCI rats.

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Section: Discussionsupporting

confidence: 69%

Section: Discussionmentioning

confidence: 99%

The Lipoxin A4 Receptor Agonist BML-111 Alleviates Inflammatory Injury and Oxidative Stress in Spinal Cord Injury

Liu

Peng

2020

Med Sci Monit

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“…Several studies have demonstrated the neuroprotective, hepatoprotective, cardioprotective, antifungal, anti-inflammatory, and anticancer activities of lycopene (Bandeira et al, 2017;Choi & Lee, 2015;Hu et al, 2017;Mariani et al, 2014;Ojha et al, 2013;Pinto, Rodríguez-Galdón, Cestero, & Macías, 2013;Shen et al, 2014;Vasconcelos et al, 2017). Lycopene pretreatment at various doses significantly delayed tumor formation and growth, thereby reducing skin carcinogenesis in female ICR mice (Shen et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Lycopene-rich extract from red guava ( Psidium guajava L.) displays cytotoxic effect against human breast adenocarcinoma cell line MCF-7 via an apoptotic-like pathway

Santos

Ombredane

Souza

et al. 2018

Food Research International

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This study investigated a lycopene-rich extract from red guava (LEG) for its chemical composition using spectrophotometry, mass spectrometry, attenuated total reflectance-fourier transform infrared spectroscopy (ATR-FTIR), and computational studies. The cytotoxic activity of LEG and the underlying mechanism was studied in human breast adenocarcinoma cells (MCF-7), murine fibroblast cells (NIH-3T3), BALB/c murine peritoneal macrophages, and sheep blood erythrocytes by evaluating the cell viability with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) method and flow cytometry. Spectrophotometry analysis showed that LEG contained 20% of lycopene per extract dry weight. Experimental and theoretical ATR-FTIR suggests the presence of lycopene, whereas MS/MS spectra obtained after fragmentation of the molecular ion [M] of 536.4364 show fragment ions at m/z 269.2259, 375.3034, 444.3788, and 467.3658, corroborating the presence of lycopene mostly related to all-trans configuration. Treatment with LEG (1600 to 6.25μg/mL) for 24 and 72h significantly affected the viability of MCF-7 cells (mean half maximal inhibitory concentration [IC]=29.85 and 5.964μg/mL, respectively) but not NIH-3T3 cells (IC=1579 and 911.5μg/mL, respectively). Furthermore LEG at concentrations from 800 to 6.25μg/mL presented low cytotoxicity against BALB/c peritoneal macrophages (IC≥800μg/mL) and no hemolytic activity. LEG (400 and 800μg/mL) caused reduction in the cell proliferation and induced cell cycle arrest, DNA fragmentation, modifications in the mitochondrial membrane potential, and morphologic changes related to granularity and size in MCF-7 cells; however, it failed to cause any significant damage to the cell membrane or display necrosis or traditional apoptosis. In conclusion, LEG was able to induce cytostatic and cytotoxic effects on breast cancer cells probably via induction of an apoptotic-like pathway.

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“…The results of Aydin and Celik similarly showed that lycopene with an antioxidant-based effect could play an important role in inhibiting the appearance of diabetic complications [47]. The study of neuroprotective effects of lycopene in spinal cord injury in rats via antioxidative and antiapoptotic pathway which has been done by Hu and colleagues indicates that lycopene administration could improve the total antioxidant status and might have neuroprotective effects on SCI [48]. On the one hand, a research by Kuhad and colleagues on lycopene attenuates diabetes-associated cognitive decline in rats get to the result that the involvement of oxidative-nitrosative stress and peripheral inflammation in the development of cognitive impairment in diabetic animals and point towards the therapeutic potential of lycopene in diabetes-induced learning and memory impairment [21].…”

Section: Discussionmentioning

confidence: 99%

The Effects of Lycopene and Insulin on Histological Changes and the Expression Level of Bcl-2 Family Genes in the Hippocampus of Streptozotocin-Induced Diabetic Rats

Soleymaninejad

Joursaraei

Feizi

et al. 2017

Journal of Diabetes Research

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The aim of this study was to evaluate the effects of antioxidants lycopene and insulin on histological changes and expression of Bcl-2 family genes in the hippocampus of streptozotocin-induced type 1 diabetic rats. Forty-eight Wistar rats were divided into six groups of control (C), control treated with lycopene (CL), diabetic (D), diabetic treated with insulin (DI), diabetic treated with lycopene (DL), and diabetic treated with insulin and lycopene (DIL). Diabetes was induced by an injection of streptozotocin (60 mg/kg, IP), lycopene (4 mg/kg/day) was given to the lycopene treated groups as gavages, and insulin (Sc, 1-2 U/kg/day) was injected to the groups treated with insulin. The number of hippocampus neurons undergoing cell death in group D had significant differences with groups C and DIL (p < 0.001). Furthermore, insulin and lycopene alone or together reduced the expression of Bax, but increased Bcl-2 and Bcl-xL levels in DI, DL, and DIL rats, especially when compared to group D (p < 0.001). The ratios of Bax/Bcl-2 and Bax/Bcl-xL in DI, DL, and DIL rats were also reduced (p < 0.001). Our results indicate that treatment with insulin and/or lycopene contribute to the prevention of cell death by reducing the expression of proapoptotic genes and increasing the expression of antiapoptotic genes in the hippocampus.

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Neuroprotective effects of lycopene in spinal cord injury in rats via antioxidative and anti-apoptotic pathway

Cited by 45 publications

References 31 publications

The Lipoxin A4 Receptor Agonist BML-111 Alleviates Inflammatory Injury and Oxidative Stress in Spinal Cord Injury

The Lipoxin A4 Receptor Agonist BML-111 Alleviates Inflammatory Injury and Oxidative Stress in Spinal Cord Injury

Lycopene-rich extract from red guava ( Psidium guajava L.) displays cytotoxic effect against human breast adenocarcinoma cell line MCF-7 via an apoptotic-like pathway

The Effects of Lycopene and Insulin on Histological Changes and the Expression Level of Bcl-2 Family Genes in the Hippocampus of Streptozotocin-Induced Diabetic Rats

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