Neuroprotective effects of Peltophorum pterocarpum leaf extract against hydrogen peroxide induced oxidative stress and cytotoxicity

Abstract

Background: Peltophorum pterocarpum is a plant from the family of Leguminosae and a tropical tree found in South East Asia region. The leaves of the plant is rich in anti-oxidant polyphenols. Traditionally this plant was used to cure intestinal disorders and as a relief for sprain, bruise, muscular pain and sores and for pain during child birth. Since oxidative stress is a major contributing factor towards neurodegenerative diseases the leaves of this plant was explored for its neuroprotective properties. Method: In this study, we report the neuroprotective properties of the ethanolic extract of Peltophorum pterocarpum leaf against oxidative stress induced cell death by H 2 O 2 , in an in vitro model of neuronally differentiated IMR32 neuroblastoma cells. Hydrogen peroxide was used as an inducer for oxidative stress. Cell viability was determined using MTT assay, apoptosis studies were carried out using Annexin V-FITC and Caspase 3 assays, mitochondrial membrane potential was examined using JC1 staining. The oxidative stress induced by H 2 O 2 was determined by quantifying the intracellular reactive oxygen species production using DCF-DA staining. Mitogen activated protein kinase signaling pathway of the neuroprotective effect of PTE was also elucidated since oxidative stress activates this pathway signaling.

Neurotoxic Agent-Induced Injury in Neurodegenerative Disease Model: Focus on Involvement of Glutamate Receptors

“…Cell lines, including BV-2 microglia, PC12 cells, and SH-SY5Y cells, are ideal for studying H 2 O 2 -induced neurotoxicity. In experimental models, H 2 O 2 -induced neurotoxicity causes mitochondrial dysfunction, oxidative stress, cytotoxicity, apoptosis, and neuronal cell death ( Han et al, 2014 ; Morelli et al, 2014 ; Ismail et al, 2015 ; Mesbah-Ardakani et al, 2016 ; Zhong et al, 2016 ; Masilamani et al, 2017 ; Oh et al, 2017 ) while it also modulates glutamate receptors. Exposure to H 2 O 2 can activate normally silent NMDARs, potentially via the inhibition of redox-sensitive glutamate uptake.…”

Catalytic degradation of methylene blue dye and antibacterial activity of biosynthesized silver nanoparticles using Peltophorum pterocarpum (DC.) leaves

“…[13][14][15][16] Other chemical constituents of PPLE have also been identied as phenoxychromones, naringenins, ophioglonins, kaempferol, luteolin, isohamnetin, chrysoeriol, quercetins, and pachypodol, 11,[17][18][19] which are generally polyphenols with strong antioxidant activities hence they account for the health benets associated with the use of PPLE. 20 The chemical constituents of PPLE make it a potential source of reducing and stabilizing agents for the green synthesis of nanoparticles (NPs). 21,22 NPs are currently included in many commercial products and as such, the use of nature-based reducing and stabilizing agents is essential in their production to ensure a reduction in toxicity and environmental hazard associated with the use of conventional synthetic chemicals in the production.…”