Neuroprotective efficacy of berberine following developmental exposure to chlorpyrifos in F1 generation of Wistar rats: Apoptosis-autophagy interplay

Seth, Era; Chopra, Mani

doi:10.1016/j.scitotenv.2022.155292

Cited by 11 publications

(6 citation statements)

References 75 publications

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“…Beside pesticides, pollutants such as flame retardants (PBDE47, TOCP) also display similar effects in vivo (rats, hens) and in SH-SY5Y cells, on the activation of the apoptosis and autophagy pathways [179,187]. Moreover, the caspase-3 dependent apoptosis induced by dieldrin, CPF, TCDD, and PFOS observed with undifferentiated SH-SY5Y was confirmed in many studies performed in more complex models such as dopaminergic cells in cultures (SN4741) and rodents' brains [215][216][217][218][219]. Altogether, these studies suggest that undifferentiated SH-SY5Y cells may represent a good model for studying neurotoxicity.…”

Section: Discussionmentioning

confidence: 74%

The SH-SY5Y human neuroblastoma cell line, a relevant in vitro cell model for investigating neurotoxicology in human: Focus on organic pollutants

et al. 2022

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Section: Discussionmentioning

confidence: 74%

The SH-SY5Y human neuroblastoma cell line, a relevant in vitro cell model for investigating neurotoxicology in human: Focus on organic pollutants

et al. 2022

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“…Oxidative stress-induced mitochondrial damage can potentially activate caspases which are the primary markers of neuronal apoptosis or necrosis [ 32 – 34 ]. Therefore, we further examined the expression levels of several main apoptosis executors, including Bcl-2, Bax and caspase-3.…”

Section: Resultsmentioning

confidence: 99%

Brain-targeted nanoreactors prevent the development of organophosphate-induced delayed neurological damage

Zou,

Wang,

et al. 2023

J Nanobiotechnol

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Background Organophosphate (OP)-induced delayed neurological damage is attributed to permanent neuropathological lesions caused by irreversible OP-neurocyte interactions, without potent brain-targeted etiological antidotes to date. The development of alternative therapies to achieve intracerebral OP detoxification is urgently needed. Methods We designed a brain-targeted nanoreactor by integrating enzyme immobilization and biomimetic membrane camouflaging protocols with careful characterization, and then examined its blood–brain barrier (BBB) permeability both in vitro and in vivo. Subsequently, the oxidative stress parameters, neuroinflammatory factors, apoptotic proteins and histopathological changes were measured and neurobehavioral tests were performed. Results The well-characterized nanoreactors exerted favourable BBB penetration capability both in vitro and in vivo, significantly inhibiting OP-induced intracerebral damage. At the cellular and tissue levels, nanoreactors obviously blocked oxidative stress, cellular apoptosis, inflammatory reactions and brain histopathological damage. Furthermore, nanoreactors radically prevented the occurrence of OP-induced delayed cognitive deficits and psychiatric abnormality. Conclusion The nanoreactors significantly prevented the development of OP-induced delayed neurological damage, suggesting a potential brain-targeted etiological strategy to attenuate OP-related delayed neurological and neurobehavioral disorders. Graphical Abstract

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“…Some previous studies have also reported that BBR is effective against oxidative stress-mediated bimolecular alterations following doxorubicin [31] and chlorpyrifos exposure. [55,56] Endogenous antioxidants are cellular tools for defense against disrupted redox balance and oxidative damage to lipids and proteins.…”

Section: Discussionmentioning

confidence: 99%

“…Some previous studies have also reported that BBR is effective against oxidative stress‐mediated bimolecular alterations following doxorubicin [ 31 ] and chlorpyrifos exposure. [ 55,56 ]…”

Section: Discussionmentioning

confidence: 99%

Neuroprotective potential of berberine against acetamiprid induced toxicity in rats: Implication of oxidative stress, mitochondrial alterations, and structural changes in brain regions

Phogat

Singh

Malik

et al. 2023

J Biochem & Molecular Tox

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Acetamiprid (ACMP) is an extensively used neonicotinoid pesticide to control sucking and chewing insects and is known to cause nontarget toxicity. The present study aimed to evaluate the ameliorative potential of berberine (BBR)—a polyphenolic alkaloid‐ on ACMP‐induced oxidative stress, mitochondrial dysfunctioning, and structural changes in different rat brain regions. The male Wistar rats were divided into four groups, that is, control, BBR‐treated (150 mg/kg b.wt), ACMP‐exposed (21.7 mg/kg b.wt) and BBR + ACMP co‐treated; and were dosed intragastrically for 21 consecutive days. Results of the biochemical analysis showed that BBR significantly ameliorated ACMP‐induced oxidative stress by decreasing lipid peroxidation and protein oxidation along with a marked increase in endogenous antioxidants and lowered AChE activity in rat brain regions. Inside mitochondria, BBR significantly attenuated the toxic effects of ACMP by increasing the activity of mitochondrial complexes. Findings of polymerase chain reaction also demonstrated the modulatory effects of BBR against ACMP‐mediated downregulation of ND1, ND2, COX1, and COX4 subunits of mitochondrial complexes. The histopathological and ultrastructural examination also validated the biochemical and transcriptional alterations following toxicity of ACMP exposure and the protective potential of BBR against ACMP‐induced neurotoxicity. Thus, the present study indicates the promising ameliorative potential of BBR against ACMP‐induced neurotoxicity via its antioxidative and modulatory activities.

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Neuroprotective efficacy of berberine following developmental exposure to chlorpyrifos in F1 generation of Wistar rats: Apoptosis-autophagy interplay

Cited by 11 publications

References 75 publications

The SH-SY5Y human neuroblastoma cell line, a relevant in vitro cell model for investigating neurotoxicology in human: Focus on organic pollutants

The SH-SY5Y human neuroblastoma cell line, a relevant in vitro cell model for investigating neurotoxicology in human: Focus on organic pollutants

Brain-targeted nanoreactors prevent the development of organophosphate-induced delayed neurological damage

Neuroprotective potential of berberine against acetamiprid induced toxicity in rats: Implication of oxidative stress, mitochondrial alterations, and structural changes in brain regions

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