Neuroprotective investigation of tanshinone in the cerebral infarction model in the Keap1-Nrf2/ARE pathway

Lee, Seonjoo; He, Liyu; Gao, Wei; Zhao, Zhiyuan

doi:10.1080/15384101.2022.2119687

Cited by 7 publications

(2 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In TLR2-mediated macrophage immune responses, TRIM13 uses K29linked polyubiquitin chains to ubiquitinate TRAF6, promoting NF-κB activity and enhancing the activation of macrophage immune responses [12]. In addition, the literature reports the inhibitory effect of TRIM13 on the proliferation of lung cancer cells [24]. TRIM13 contributes to the development of kidney diseases.…”

Section: Discussionmentioning

confidence: 99%

Inhibition of ALKBH5 Inhibits Inflammation and Excessive Proliferation by Alleviating TRIM13 m6A Modifications in Glomerular Mesangial Cells

Hu,

Liu,

Zhuang

et al. 2023

Preprint

View full text Add to dashboard Cite

Objectives; Chronic glomerulonephritis (CGN) refers to the inflammation of glomeruli in the kidneys. Glomerular mesangial cells (GMCs) play a pivotal role in the development of CGN. In the present study, we investigated the impact of ALKBH5, a m6A demethylase, on inflammation and hyperproliferation in mouse glomerular mesangial cells (MMCs) and elucidated the molecular mechanisms contributing to CGN. Materials and methods; Western blotting and reverse transcriptase-polymerase chain reaction (RT-qPCR) were employed to evaluate the expression of relevant genes, including ALKBH5 and TRIM13. In addition, enzyme-linked immunosorbent assay (ELISA) was used to measure the levels of inflammatory factors (interleukin [IL]-1β, tumor necrosis factor [TNF]-α, and IL-10) in the lipopolysaccharide (LPS)-induced MMCs supernatant. Methylated RNA immunoprecipitation (MeRIP) was performed to investigate the effect of ALKBH5 on the levels of TRIM13-m6A mRNA. The stability of TRIM13 mRNA was evaluated using an actinomycin D assay. Results; Significantly elevated expression of ALKBH5 was found in LPS-induced MMCs. Interference with ALKBH5 expression inhibited inflammation and excessive proliferation in LPS-induced MMCs. Moreover, interfering with ALKBH5 expression significantly reduced the levels of TRIM13-m6A modification. The overexpression of TRIM13 in MMCs reversed the inflammation and proliferation induced by ALKBH5 interference. In addition, interference with TRIM13 expression inhibited the activation of the nuclear factor (NF)-κB pathway and suppressed inflammation and proliferation in MMCs. Conclusion; Inhibiting ALKBH5 hinders inflammation and hyperproliferation by improving TRIM13-m6A modification in glomerular MCs. We believe these findings will further provide insights into the molecular mechanisms and potential therapeutic targets for CGN.

show abstract

Section: Discussionmentioning

confidence: 99%

Inhibition of ALKBH5 Inhibits Inflammation and Excessive Proliferation by Alleviating TRIM13 m6A Modifications in Glomerular Mesangial Cells

Hu,

Liu,

Zhuang

et al. 2023

Preprint

View full text Add to dashboard Cite

show abstract

“…Nrf2 is a vital inducer of nuclear transcription [61] that controls the transcription of many antioxidant genes [62] . In this way, Nrf2 is the most important endogenous antioxidant stress pathway [63][64] . Recent reports have illustrated a relationship between Nrf2 and cataracts [37] .…”

Section: Potential Mechanism For the Effect Of Aluas Rna On Mgo-cause...mentioning

confidence: 99%

Alu antisense RNA ameliorates methylglyoxal-induced human lens epithelial cell apoptosis by enhancing antioxidant defense

Wu¹,

Wu²,

Wang³

et al. 2023

Int J Ophthalmol

View full text Add to dashboard Cite

AIM: To determine whether an antisense RNA corresponding to the human Alu transposable element (Aluas RNA) can protect human lens epithelial cells (HLECs) from methylglyoxal-induced apoptosis. METHODS: Cell counting kit-8 (CCK-8) and 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assays were used to assess HLEC viability. HLEC viability/death was detected using a Calcein-AM/PI double staining kit; the annexin V-FITC method was used to detect HLEC apoptosis. The cytosolic reactive oxygen species (ROS) levels in HLECs were determined using a reactive species assay kit. The levels of malondialdehyde (MDA) and the antioxidant activities of total-superoxide dismutase (T-SOD) and glutathione peroxidase (GSH-Px) were assessed in HLECs using their respective kits. RT-qPCR and Western blotting were used to measure mRNA and protein expression levels of the genes. RESULTS: Aluas RNA rescued methylglyoxal-induced apoptosis in HLECs and ameliorated both the methylglyoxal-induced decrease in Bcl-2 mRNA and the methylglyoxal-induced increase in Bax mRNA. In addition, Aluas RNA inhibited the methylglyoxal-induced increase in Alu sense RNA expression. Aluas RNA inhibited the production of ROS induced by methylglyoxal, restored T-SOD and GSH-Px activity, and moderated the increase in MDA content after treatment with methylglyoxal. Aluas RNA significantly restored the methylglyoxal-induced down-regulation of Nrf2 gene and antioxidant defense genes, including glutathione peroxidase, heme oxygenase 1, γ-glutamylcysteine synthetase and quinone oxidoreductase 1. Aluas RNA ameliorated methylglyoxal-induced increases of the mRNA and protein expression of Keap1 that is the negative regulator of Nrf2. CONCLUSION: Aluas RNA reduces apoptosis induced by methylglyoxal by enhancing antioxidant defense.

show abstract

Piceatannol Protects Sperm from Cryopreservation Damage by Modulating the Keap1-Nrf2/ARE Signaling Pathway

Fu,

Wang,

et al. 2024

Reprod. Sci.

View full text Add to dashboard Cite

Neuroprotective investigation of tanshinone in the cerebral infarction model in the Keap1-Nrf2/ARE pathway

Cited by 7 publications

References 32 publications

Inhibition of ALKBH5 Inhibits Inflammation and Excessive Proliferation by Alleviating TRIM13 m6A Modifications in Glomerular Mesangial Cells

Inhibition of ALKBH5 Inhibits Inflammation and Excessive Proliferation by Alleviating TRIM13 m6A Modifications in Glomerular Mesangial Cells

Alu antisense RNA ameliorates methylglyoxal-induced human lens epithelial cell apoptosis by enhancing antioxidant defense

Piceatannol Protects Sperm from Cryopreservation Damage by Modulating the Keap1-Nrf2/ARE Signaling Pathway

Contact Info

Product

Resources

About