Neuroprotective potential of terpenoid-rich extracts from orange juice by-products obtained by pressurized liquid extraction

Sánchez-Martínez, José David; Álvarez-Rivera, Gerardo; Gallego, Rocío; Fagundes, Mariane Bittencourt; Valdés, Alberto; Mendiola, J. A.

doi:10.1016/j.fochx.2022.100242

Cited by 17 publications

(20 citation statements)

References 39 publications

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“…These results were slightly worse than for the positive control (quercetin, IC 50 of 17.2 µg/mL), so the IC 50 values of the FD, HAD90 and ND extracts can be considered as moderate (25–100 µg/mL [ 33 ]). These results were similar to those obtained from orange juice by-product extracts [ 16 , 23 ], which have shown promising neuroprotective potential. Other studies have also demonstrated the anti-inflammatory properties of different natural products extracts, suggesting that the phenolic compounds are responsible for these effects [ 33 ].…”

Section: Resultssupporting

confidence: 87%

“…The PLE conditions were optimized using a CCD, as described in Section 2.4 , and by selecting the TPC, AChE and LOX inhibitory activity levels and ROS scavenging capacity as response variables. Since previous studies had demonstrated that the extraction yield is not necessarily connected to the neuroprotective potential of different natural extracts [ 22 , 23 ], this response variable was not considered in the PLE optimization model. Moreover, and considering that phenolic compounds can prevent the development of the AD pathology [ 24 , 25 , 26 ], the TPC was fostered in the optimization process.…”

Section: Resultsmentioning

confidence: 99%

“…On the other hand, the lowest activity was found in the ND extracts. Previous studies have shown that PLE extracts obtained from different natural sources have high antioxidant capacity based on their phenolic composition [ 22 , 23 ]. Phenolic compounds are characterized by possessing phenolic hydroxyl groups that are prone to donate a hydrogen atom or an electron to a free radical, and they have an extended conjugated aromatic system that is used to delocalize an unpaired ROS or RNS electron [ 38 ].…”

Section: Resultsmentioning

confidence: 99%

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Neuroprotective Potential of Thinned Peaches Extracts Obtained by Pressurized Liquid Extraction after Different Drying Processes

Guo

Valdés

Sánchez-Martínez

et al. 2022

Foods

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Genetic, environmental and nutritional factors are suggested as primary factors of Alzheimer’s disease (AD), and secondary metabolites such as polyphenols present in thinned peaches are considered as good candidates for AD prevention. Thinned peaches are usually dried to avoid putrefaction, but the effects of the drying method and the extraction process on the polyphenol composition and the neuroprotective potential have never been addressed. In this work, a pressurized liquid extraction (PLE) method was optimized and applied to thinned peaches dried under different conditions, and their neuroprotective potential was evaluated in vitro. In addition, the PLE extracts were characterized via HPLC-Q-TOF-MS/MS, and a permeability assay was performed to evaluate the ability of the identified metabolites to cross the blood–brain barrier (BBB). The PLE extracts obtained from freeze-dried (FD) samples with 50% ethanol in water at 180 °C showed the best neuroprotective potential. Finally, among the 81 metabolites identified, isoferulic acid, 4-methyldaphnetin, coniferyl aldehyde and 3,4-dihydroxyacetophenone were found at higher concentrations in FD extracts. These metabolites are able to cross the BBB and are positively correlated with the neuroprotective potential, suggesting FD together with PLE extraction as the best combination to exploit the neuroprotective capacity of thinned peaches.

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Section: Resultssupporting

confidence: 87%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

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Neuroprotective Potential of Thinned Peaches Extracts Obtained by Pressurized Liquid Extraction after Different Drying Processes

Guo

Valdés

Sánchez-Martínez

et al. 2022

Foods

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“…Nonetheless, other components of EDAC may also play an important role, and two of them, methyl hexadecanoate and γ-sitosterol, have relevant pharmacological potential in the CNS. For example, methyl hexadecanoate has been shown to improve neuronal survival in the CA1 region of the hippocampus, as well as recovery of learning and memory, in a rat model of global cerebral ischemia [34], and γ-sitosterol was identi ed in neuroprotective terpenoid-rich extracts from orange juice [35]. Further studies are required to unravel the relative contributions of EDAC constituents to cytoprotection in the CNS, and although it is likely that coumarin is of major importance in the effects observed in the present study, an exciting possibility is that the multiple compounds in EDAC have combinatorial or even synergistic effects.…”

Section: Discussionmentioning

confidence: 99%

Amburana cearensis seed extract stimulates astrocyte glutamate homeostatic mechanisms in hippocampal brain slices and protects oligodendrocytes against ischemia

Ferreira

Ribeiro

Silva

et al. 2022

Preprint

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Background. Stroke is a leading cause of death and disability worldwide. A major factor in brain damage following ischemia is excitotoxicity caused by elevated levels of the neurotransmitter glutamate. In the brain, glutamate homeostasis is a primary function of astrocytes. Amburana cearensis has long been used in folk medicine and seed extract obtained with dichloromethane (EDAC) have previously been shown to exhibit cytoprotective activity in vitro. The aim of the present study was to analyse the activity of EDAC in hippocampal brain slices. Methods. We prepared a dichloromethane extract (EDAC) from A. cearensis seeds and characterized the chemical constituents by 1H and 13C-NMR. Hippocampal slices from P6-8 or P90 Wistar rats were used for cell viability assay or glutamate uptake test. Hippocampal slices from P10-12 transgenic mice SOX10-EGFP and GFAP-EGFP and immunofluorescence for GS, GLAST and GLT1 were used to study oligodendrocytes and astrocytes. Results. Astrocytes play a critical role in glutamate homeostasis and we provide immunohistochemical evidence that in excitotoxicity EDAC increased expression of glutamate transporters and glutamine synthetase, which is essential for detoxifying glutamate. Next, we directly examined astrocytes using transgenic mice in which glial fibrillary acidic protein (GFAP) drives expression of enhanced green fluorescence protein (EGFP) and show that glutamate excitotoxicity caused a decrease in GFAP-EGFP and that EDAC protected against this loss. This was examined further in the oxygen-glucose deprivation (OGD) model of ischemia, where EDAC caused an increase in astrocytic process branching, resulting in an increase in GFAP-EGFP. Using SOX10-EGFP reporter mice, we show that the acute response of oligodendrocytes to OGD in hippocampal slices is a marked loss of their processes and EDAC protected oligodendrocytes against this damage. Conclusion. This study provides evidence that EDAC is cytoprotective against ischemia and glutamate excitotoxicity by modulating astrocyte responses and stimulating their glutamate homeostatic mechanisms.

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“…However, the extraction efficiency may be improved when it is used coupled to other technologies such as the microwave, enzyme, or ultrasound, being that it is necessary to adjust the conditions of both methodologies to obtain targeted compounds [ 35 , 37 , 86 ]. Due to the fact that PLE is performed in equipment that usually sets the working pressure at 100–110 bars [ 11 , 75 ], temperature and solvent composition are the factors that exert a major effect on the evaluated responses [ 76 ]. However, the number of extraction cycles or the static extraction time are also usually evaluated [ 74 , 100 ].…”

Section: Doe: From Concept To Product Developmentmentioning

confidence: 99%

Application of Response Surface Methodologies to Optimize High-Added Value Products Developments: Cosmetic Formulations as an Example

et al. 2022

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In recent years, green and advanced extraction technologies have gained great interest to revalue several food by-products. This by-product revaluation is currently allowing the development of high value-added products, such as functional foods, nutraceuticals, or cosmeceuticals. Among the high valued-added products, cosmeceuticals are innovative cosmetic formulations which have incorporated bioactive natural ingredients providing multiple benefits on skin health. In this context, the extraction techniques are an important step during the elaboration of cosmetic ingredients since they represent the beginning of the formulation process and have a great influence on the quality of the final product. Indeed, these technologies are claimed as efficient methods to retrieve bioactive compounds from natural sources in terms of resource utilization, environmental impact, and costs. This review offers a summary of the most-used green and advanced methodologies to obtain cosmetic ingredients with the maximum performance of these extraction techniques. Response surface methodologies may be applied to enhance the optimization processes, providing a simple way to understand the extraction process as well as to reach the optimum conditions to increase the extraction efficiency. The combination of both assumes an economic improvement to attain high value products that may be applied to develop functional ingredients for cosmetics purposes.

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Neuroprotective potential of terpenoid-rich extracts from orange juice by-products obtained by pressurized liquid extraction

Cited by 17 publications

References 39 publications

Neuroprotective Potential of Thinned Peaches Extracts Obtained by Pressurized Liquid Extraction after Different Drying Processes

Neuroprotective Potential of Thinned Peaches Extracts Obtained by Pressurized Liquid Extraction after Different Drying Processes

Amburana cearensis seed extract stimulates astrocyte glutamate homeostatic mechanisms in hippocampal brain slices and protects oligodendrocytes against ischemia

Application of Response Surface Methodologies to Optimize High-Added Value Products Developments: Cosmetic Formulations as an Example

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