Neuroprotective Role of Trolox in Hippocampus after Ischemia                     Reperfusion Injury in Mouse

Sarveazad, Arash; Babahajian, Asrin; Yari, Abazar; Goudarzi, Farjam; Soleimani, Mansoureh; Nourani, Mohammadreza

doi:10.1024/0300-9831/a000293

Cited by 7 publications

(6 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…[ 18 19 20 ] In cultured bursal cell and neuronal studies, trolox protected against cell death resulting from oxidative stress. [ 21 ] Trolox has an antioxidant role in reducing apoptosis in mouse thymocytes, rabbit myocytes, and anterior pituitary cells. [ 22 23 ]…”

Section: Introductionmentioning

confidence: 99%

Moderate Dose of Trolox Preventing the Deleterious Effects of Wi-Fi Radiation on Spermatozoa In vitro through Reduction of Oxidative Stress Damage

Ding

Sun

Zhang

et al. 2018

Chinese Medical Journal

View full text Add to dashboard Cite

Background:The worsening of semen quality, due to the application of Wi-Fi, can be ameliorated by Vitamin E. This study aimed to demonstrate whether a moderate dose of trolox, a new Vitamin E, inhibits oxidative damage on sperms in vitro after exposure to Wi-Fi radiation.Methods:Each of the twenty qualified semen, gathered from June to October 2014 in eugenics clinic, was separated into four aliquots, including sham, Wi-Fi-exposed, Wi-Fi plus 5 mmol/L trolox, and Wi-Fi plus 10 mmol/L trolox groups. At 0 min, all baseline parameters of the 20 samples were measured in sequence. Reactive oxygen species, glutathione, and superoxide dismutase were evaluated in the four aliquots at 45 and 90 min, as were sperm DNA fragments, sperm mitochondrial potential, relative amplification of sperm mitochondrial DNA, sperm vitality, and progressive and immotility sperm. The parameters were analyzed by one-way analysis of variance and Tukey's posttest.Results:Among Wi-Fi plus 5 mmol/L trolox, Wi-Fi-exposed and Wi-Fi plus 10 mmol/L trolox groups, reactive oxygen species levels (45 min: 3.80 ± 0.41 RLU·10−6·ml−1 vs. 7.50 ± 0.35 RLU·10−6·ml−1 vs. 6.70 ± 0.47 RLU·10−6·ml−1, P < 0.001; 90 min: 5.40 ± 0.21 RLU·10−6·ml−1 vs. 10.10 ± 0.31 RLU·10−6·ml−1 vs. 7.00 ± 0.42 RLU·10−6·ml−1, P < 0.001, respectively), percentages of tail DNA (45 min: 16.8 ± 2.0% vs. 31.9 ± 2.5% vs. 61.3 ± 1.6%, P < 0.001; 90 min: 19.7 ± 1.5% vs. 73.7 ± 1.3% vs. 73.1 ± 1.1%, P < 0.001, respectively), 8-hydroxy-2’-deoxyguanosine (45 min: 51.89 ± 1.46 pg/ml vs. 104.89 ± 2.19 pg/ml vs. 106.11 ± 1.81 pg/ml, P = 0.012; 90 min: 79.96 ± 1.73 pg/ml vs. 141.73 ± 2.90 pg/ml vs. 139.06 ± 2.79 pg/ml; P < 0.001), and percentages of immotility sperm (45 min: 27.7 ± 2.7% vs. 41.7 ± 2.2% vs. 41.7 ± 2.5%; 90 min: 29.9 ± 3.3% vs. 58.9 ± 4.0% vs. 63.1 ± 4.0%; all P < 0.001) were lowest, and glutathione peroxidase (45 min: 60.50 ± 1.54 U/ml vs. 37.09 ± 1.77 U/ml vs. 28.18 ± 1.06 U/ml; 90 min: 44.61 ± 1.23 U/ml vs. 16.86 ± 0.93 U/ml vs. 29.94 ± 1.56 U/ml; all P < 0.001), percentages of head DNA (45 min: 83.2 ± 2.0% vs. 68.2 ± 2.5% vs. 38.8 ± 1.6%; 90 min: 80.3 ± 1.5% vs. 26.3 ± 1.3% vs. 26.9 ± 1.1%; all P < 0.001), percentages of sperm vitality (45 min: 89.5 ± 1.6% vs. 70.7 ± 3.1% vs. 57.7 ± 2.4%; 90 min: 80.8 ± 2.2% vs. 40.4 ± 4.0% vs. 34.7 ± 3.9%; all P < 0.001), and progressive sperm (45 min: 69.3 ± 2.7% vs. 55.8 ± 2.2% vs. 55.4 ± 2.5%; 90 min: 67.2 ± 3.3% vs. 38.2 ± 4.0% vs. 33.9 ± 4.0%; all P < 0.001) were highest in Wi-Fi plus 5 mmol/L trolox group at 45 and 90 min, respectively. Other parameters were not affected, while the sham group maintained the baseline.Conclusion:This study found that 5 mmol/L trolox protected the Wi-Fi-exposed semen in vitro from the damage of electromagnetic radiation-induced oxidative stress.

show abstract

Section: Introductionmentioning

confidence: 99%

Moderate Dose of Trolox Preventing the Deleterious Effects of Wi-Fi Radiation on Spermatozoa In vitro through Reduction of Oxidative Stress Damage

Ding

Sun

Zhang

et al. 2018

Chinese Medical Journal

View full text Add to dashboard Cite

show abstract

“…Previous studies demonstrated that trolox can scavenge excess ROS and act as an antioxidant therapy in myocardial injury and diabetic retinopathy (Diaz et al, 2005). Trolox also exhibits a preventive effect on oxidative stress-induced cell death (Sarveazad et al, 2016) and apoptosis in thymocytes, cardiomyocytes, and anterior pituitary cells (Poliandri et al, 2003). This study focused on the anti-aging effect of trolox in MSCs culture.…”

Section: Discussionmentioning

confidence: 99%

The antioxidant trolox inhibits aging and enhances prostaglandin E-2 secretion in mesenchymal stem cells

ZHANG¹,

Zhang²,

DU³

et al. 2023

Biocell

View full text Add to dashboard Cite

Mesenchymal stem cells (MSCs) have been widely used in regenerative medicine and clinical therapy due to their capabilities of proliferation, differentiation, and immune regulation. However, during in vitro expansion, MSCs are prone to aging, which largely limits their application. Prostaglandin E-2 (PGE-2) is a key effector secreted by MSCs to exert immunomodulatory effects. By screening the compound library for PGE-2 secretion, the antioxidant trolox was verified as a stimulator of MSCs to secrete PGE-2. The effect of antioxidant trolox on biological characteristics of MSCS, including aging, proliferation, and gene expression, was examined. The results demonstrated that trolox can resist aging, promote proliferation, and enhance PGE-2 secretion of MSCs without affecting their surface marker expression. Furthermore, trolox treatment up-regulates miR-17-92 clusters in MSCs and may contribute to its anti-aging effects. Thus, trolox addition might be beneficial for MSCs expansion and their application.

show abstract

“…The Injection speed was 0.25 µlit/min. Mice successfully modeled were randomly assigned (Sarveazad, Babahajian et al 2017) in equal numbers to ve groups (n = 10 per group) as follows:…”

Section: Transplantation Of Dps Cells Into Hippocampusmentioning

confidence: 99%

The neuroprotective effects of dental pulp stem cells and erythropoietin in mice hippocampus after ischemia-reperfusion

Poor

Moghadam

Rezayof

et al. 2022

Preprint

Self Cite

View full text Add to dashboard Cite

Background Brain strokes are the cause of death in many people, among survivors; it can cause problems such as motor and cognitive impairment. The role of the hippocampus and its damage in ischemia has been assessed by researchers. One of the treatments commonly used today by researchers in cell therapy. Therefore, this study aimed to evaluate the use of dental pulp stem cells and erythropoietin in mice hippocampus after ischemia-reperfusion. Methods In this study, NMRI male mice were divided into six groups. Except for the sham group, all groups group experienced ischemic hippocampus. A group received erythropoietin or dental pulp stem cells and the other group received a combination exposer of erythropoietin and DPSC, while the vehicle group received DPSC solvent and erythropoietin solvent. After eight weeks, they were subjected to a test of learning and memory by Morris water maze. Then, their brains were examined for histological assessment, and immunohistochemistry (DCX and NeuN for neurogenesis). Furthermore, VEGF was applied for angiogenesis and GFAP for gliosis examination. Results The behavioral function of the group receiving erythropoietin and the combined group (DPSC and erythropoietin) was better than other groups. The mean number of healthy cells in EPO, DPSC, and EPO + DPSC groups was significantly different from that of the vehicle group (P < 0.05). Besides, DPSC, EPO, and EPO + DPSC groups showed a significant increase in green density in comparison with the ischemia and vehicle groups (P < 0.05), but no difference was found between the ischemia and sham groups. Conclusion DPSC and erythropoietin were capable of increased neuronal function but behavioral studies revealed that outcomes of erythropoietin therapy are better than DPSC

show abstract

Neuroprotective Role of Trolox in Hippocampus after Ischemia Reperfusion Injury in Mouse

Cited by 7 publications

References 0 publications

Moderate Dose of Trolox Preventing the Deleterious Effects of Wi-Fi Radiation on Spermatozoa In vitro through Reduction of Oxidative Stress Damage

Moderate Dose of Trolox Preventing the Deleterious Effects of Wi-Fi Radiation on Spermatozoa In vitro through Reduction of Oxidative Stress Damage

The antioxidant trolox inhibits aging and enhances prostaglandin E-2 secretion in mesenchymal stem cells

The neuroprotective effects of dental pulp stem cells and erythropoietin in mice hippocampus after ischemia-reperfusion

Contact Info

Product

Resources

About