Neurospora crassa Female Development Requires the PACC and Other Signal Transduction Pathways, Transcription Factors, Chromatin Remodeling, Cell-To-Cell Fusion, and Autophagy

Chinnici, Jennifer; Fu, Ci; Caccamise, Lauren M.; Arnold, Jason W.; Free, Stephen J.

doi:10.1371/journal.pone.0110603

Cited by 38 publications

(44 citation statements)

References 127 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The PACC pathway was reported to be involved in glycosylation of Pi-repressible acid phosphatase [24], the transcription of the hsp70 gene [25] and the requirement for female development [26] in N . crassa .…”

Section: Introductionmentioning

confidence: 99%

Molecular Components of the Neurospora crassa pH Signaling Pathway and Their Regulation by pH and the PAC-3 Transcription Factor

et al. 2016

View full text Add to dashboard Cite

Environmental pH induces a stress response triggering a signaling pathway whose components have been identified and characterized in several fungi. Neurospora crassa shares all six components of the Aspergillus nidulans pH signaling pathway, and we investigate here their regulation during an alkaline pH stress response. We show that the N. crassa pal mutant strains, with the exception of Δpal-9, which is the A. nidulans palI homolog, exhibit low conidiation and are unable to grow at alkaline pH. Moreover, they accumulate the pigment melanin, most likely via regulation of the tyrosinase gene by the pH signaling components. The PAC-3 transcription factor binds to the tyrosinase promoter and negatively regulates its gene expression. PAC-3 also binds to all pal gene promoters, regulating their expression at normal growth pH and/or alkaline pH, which indicates a feedback regulation of PAC-3 in the pal gene expression. In addition, PAC-3 binds to the pac-3 promoter only at alkaline pH, most likely influencing the pac-3 expression at this pH suggesting that the activation of PAC-3 in N. crassa results from proteolytic processing and gene expression regulation by the pH signaling components. In N. crassa, PAC-3 is proteolytically processed in a single cleavage step predominately at alkaline pH; however, low levels of the processed protein can be observed at normal growth pH. We also demonstrate that PAC-3 preferentially localizes in the nucleus at alkaline pH stress and that the translocation may require the N. crassa importin-α since the PAC-3 nuclear localization signal (NLS) has a strong in vitro affinity with importin-α. The data presented here show that the pH signaling pathway in N. crassa shares all the components with the A. nidulans and S. cerevisiae pathways; however, it exhibits some properties not previously described in either organism.

show abstract

Section: Introductionmentioning

confidence: 99%

Molecular Components of the Neurospora crassa pH Signaling Pathway and Their Regulation by pH and the PAC-3 Transcription Factor

et al. 2016

View full text Add to dashboard Cite

show abstract

“…The fungal pH responsive regulatory domain encompasses a very large number of genes including those involved in nutrient acquisition, ion homeostasis, alkali metal and pH tolerance, cell wall metabolism, exported metabolite production, female development, sporulation, dimorphic shift, tissue penetration and invasive growth (Lamb et al, 2001;Lamb and Mitchell, 2003;Bensen et al, 2004;Eisendle et al, 2004;Baek et al, 2006;Ruiz and Ariño, 2007;Nobile et al, 2008;Alkan et al, 2013;Trushina et al, 2013;Bertuzzi et al, 2014;Chinnici et al, 2014;O'Meara et al, 2014). As many of these activities or attributes are crucial in a host environment, pH regulation is an important virulence determinant of fungal pathogenicity of animals, including humans, plants and fungi them-selves (Davis et al, 2000a;Davis, 2003;Bignell et al, 2005;Moreno-Mateos et al, 2007;Nobile et al, 2008;Zou et al, 2010;Alkan et al, 2013;Trushina et al, 2013;Bertuzzi et al, 2014;O'Meara et al, 2014) and reviewed in Peñalva et al (2008), Davis (2009), Selvig and Alspaugh (2011) and Cornet and Gaillardin (2014).…”

Section: Introductionmentioning

confidence: 99%

Refining the pH response in Aspergillus nidulans: a modulatory triad involving PacX, a novel zinc binuclear cluster protein

Bussink

Bignell

Múnera-Huertas

et al. 2015

Molecular Microbiology

View full text Add to dashboard Cite

SummaryThe A spergillus nidulans PacC transcription factor mediates gene regulation in response to alkaline ambient pH which, signalled by the Pal pathway, results in the processing of PacC72 to PacC27 via PacC53. Here we investigate two levels at which the pH regulatory system is transcriptionally moderated by pH and identify and characterise a new component of the pH regulatory machinery, PacX. Transcript level analysis and overexpression studies demonstrate that repression of acid‐expressed pal F, specifying the Pal pathway arrestin, probably by PacC27 and/or PacC53, prevents an escalating alkaline pH response. Transcript analyses using a reporter and constitutively expressed pac C trans‐alleles show that pac C preferential alkaline‐expression results from derepression by depletion of the acid‐prevalent PacC72 form. We additionally show that pac C repression requires PacX. pac X mutations suppress PacC processing recalcitrant mutations, in part, through derepressed PacC levels resulting in traces of PacC27 formed by pH‐independent proteolysis. pac X was cloned by impala transposon mutagenesis. PacX, with homologues within the Leotiomyceta, has an unusual structure with an amino‐terminal coiled‐coil and a carboxy‐terminal zinc binuclear cluster. pacX mutations indicate the importance of these regions. One mutation, an unprecedented finding in A . nidulans genetics, resulted from an insertion of an endogenous Fot1‐like transposon.

show abstract

“…Besides the effect of light on carotenoid pigmentation, light also affects pigment production in sexual reproductive structures via melanin biosynthesis pathways. Sev- eral genes and enzymes required for melanization of sexual structure have been reported (50,51). Based on initial screening of transcriptome results, we identified the genes encoding enzymes involved in the melanin biosynthesis, including scytalone dehydratase (SF_NCU07823), Sfper-1 (per-1) (polyketide synthase; SF_NCU03584), tetrahydroxynaphthalene reductase (SF_NCU06905/SF_NCU09390), and T gene (tyrosinase; SF_NCU00776) (see Table S1).…”

Section: Resultsmentioning

confidence: 99%

The Blue-Light Photoreceptor Sfwc-1 Gene Regulates the Phototropic Response and Fruiting-Body Development in the Homothallic Ascomycete Sordaria fimicola

Krobanan

Liang²,

Chiu³

et al. 2019

Appl Environ Microbiol

View full text Add to dashboard Cite

Sordaria fimicola, a coprophilous ascomycete, is a homothallic fungus that can undergo sexual differentiation with cellular and morphological changes followed by multicellular tissue development to complete its sexual cycle. In this study, we identified and characterized the blue-light photoreceptor gene in S. fimicola. The S. fimicola white collar-1 photoreceptor (SfWC-1) contains light-oxygen-voltage-sensing (LOV), Per-Arnt-Sim (PAS), and other conserved domains and is homologous to the WC-1 blue-light photoreceptor of Neurospora crassa. The LOV domain of Sfwc-1 was deleted by homologous recombination using Agrobacterium-mediated protoplast transformation. The Sfwc-1(Δlov) mutant showed normal vegetative growth but produced less carotenoid pigment under illumination. The mutant showed delayed and less-pronounced fruiting-body formation, was defective in phototropism of the perithecial beaks, and lacked the fruiting-body zonation pattern compared with the wild type under the illumination condition. Gene expression analyses supported the light-induced functions of the Sfwc-1 gene in the physiology and developmental process of perithecial formation in S. fimicola. Moreover, green fluorescent protein (GFP)-tagged SfWC-1 fluorescence signals were transiently strong upon light induction and prominently located inside the nuclei of living hyphae. Our studies focused on the putative blue-light photoreceptor in a model ascomycete and contribute to a better understanding of the photoregulatory functions and networks mediated by the evolutionarily conserved blue-light photoreceptors across diverse fungal phyla. IMPORTANCE Sordaria sp. has been a model for study of fruiting-body differentiation in fungi. Several environmental factors, including light, affect cellular and morphological changes during multicellular tissue development. Here, we created a light-oxygen-voltage-sensing (LOV) domain-deleted Sfwc-1 mutant to study blue-light photoresponses in Sordaria fimicola. Phototropism and rhythmic zonation of perithecia were defective in the Sfwc-1(Δlov) mutant. Moreover, fruiting-body development in the mutant was reduced and also significantly delayed. Gene expression analysis and subcellular localization study further revealed the light-induced differential gene expression and cellular responses upon light stimulation in S. fimicola.

show abstract

Neurospora crassa Female Development Requires the PACC and Other Signal Transduction Pathways, Transcription Factors, Chromatin Remodeling, Cell-To-Cell Fusion, and Autophagy

Cited by 38 publications

References 127 publications

Molecular Components of the Neurospora crassa pH Signaling Pathway and Their Regulation by pH and the PAC-3 Transcription Factor

Molecular Components of the Neurospora crassa pH Signaling Pathway and Their Regulation by pH and the PAC-3 Transcription Factor

Refining the pH response in Aspergillus nidulans: a modulatory triad involving PacX, a novel zinc binuclear cluster protein

The Blue-Light Photoreceptor Sfwc-1 Gene Regulates the Phototropic Response and Fruiting-Body Development in the Homothallic Ascomycete Sordaria fimicola

Contact Info

Product

Resources

About