Neurosteroids and their receptors in ischemic stroke: From molecular mechanisms to therapeutic opportunities

Vahidinia, Zeinab; Karimian, Mohammad; Joghataei, Mohammad Taghi

doi:10.1016/j.phrs.2020.105163

Cited by 26 publications

(16 citation statements)

References 340 publications

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“…In addition, vitamin transport proteins were also found to be enriched to a high degree. Notably, it had been previously found that vitamins, including vitamin D and vitamin A, can have neuroprotective effects, which can be mediated through various signalling pathways [ 31 ]. Interestingly, vitamins have also been related to immune responses [ 32 , 33 ].…”

Section: Discussionmentioning

confidence: 99%

“…Therefore, interesting questions were raised regarding whether ZRS has an effect on vitamin transport and thus modulate the immune system to contribute to brain damage repair. Studies have shown that activation of vitamin receptors can affect various processes, including immune modulation, inflammation and detoxification [ 31 ]. Vitamin D hormone can stimulate transforming growth factor TGFβ-1 and interleukin 4 (IL-4) production, which in turn may suppress inflammatory T cell activity [ 32 ].…”

Section: Discussionmentioning

confidence: 99%

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The proteomics analysis of the effects of Zhishi Rhubarb soup on ischaemic stroke

et al. 2021

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Background Stroke has always been a major threat worldwide but is most severe in China, with 2.5 million new stroke cases each year and 7.5 million stroke survivors, placing a heavy burden on the social and national health care systems. Zhishi Rhubarb Soup (ZRS) is a traditional Chinese medicine (TCM) that has been used clinically for many years in China. To explore the potential mechanism of ZRS in the treatment of stroke, liquid chromatography with mass spectrometry (LC–MS) was performed. Methods In this study, a quantitative proteomic method with LC–MS was used to analyse the proteomic differences between MACO samples treated with ZRS and those without ZRS treatment. Results Liquid chromatography with mass spectrometry (LC–MS) analysis led to the identification of 35,006 peptides, with 5160.0 proteins identified and 4094.0 quantified. Significantly differentially expressed proteins were identified through data analysis, and the difference was found to be more than 1.2 times (P < 0.05). The Gene Ontology (GO) analysis provided a summary of the dysregulated protein expression in the biological process (BP), cell component (CC), and molecular function (MF) categories. Proteins related to brain repair, including BDNF, IL-10, IL-6, and TGF-β, were found to change significantly, partially demonstrating the effectiveness of ZRS to attenuate tissue injury. Conclusion In this study, LC–MS/MS was performed to assess the effects of ZRS on differentially expressed proteins in rats with cerebral infarction. These promising results could help to improve the understanding of the effects of drugs on stroke.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

The proteomics analysis of the effects of Zhishi Rhubarb soup on ischaemic stroke

et al. 2021

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show abstract

“…4B). Noteworthy is that it was previously found that vitamin, including vitamin D and vitamin A, could have a neuroprotective effect, which could be mediated through various signaling pathways 31 . Interestingly, vitamins are found to be related to immune responses 32,33 .…”

Section: Discussionmentioning

confidence: 99%

“…Therefore, interesting questions could be raised whether ZRS could have some effects on the vitamin transportations and thus modulate the immune system to contribute the brain repair. Studies showed that activation of vitamin receptors could affect various processes including immune modulation, in ammation and detoxi cation 31 . Vitamin D hormone could stimulate transforming growth factor TGFβ-1 and interleukin 4 (IL-4) production, which in turn may suppress in ammatory T cell activity 32 .…”

Section: Discussionmentioning

confidence: 99%

The Proteomics Analysis of the Effects of Zhishi Rhubarb Soup on Ischemic Stroke

ZHANG

SHAO

HUI

et al. 2021

Preprint

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Background: Stroke has always been a big threat around the world, but most severe in China with 2.5 million new stroke cases each year and 7.5 million stroke survivors, which also has laid a heavy burden on social and national healthcare system. The Zhishi Rhubarb Soup (ZRS) is a Traditional Chinese Medicine (TCM) that has been used clinically for many years in China. In order to explore the potential mechanism of ZRS as the pathogen of stroke. In this study, liquid chromatography mass spectrometry (LC-MS) was employed to uncover the mechanism underlying the effects of ZRS on stroke.Methods: In this study, the quantitative proteomics method LC-MS was used to analyze the proteomic differences of middle cerebral artery occlusion MCAO between normal and after ZSR treatment. Results: Further liquid chromatography mass spectrometry (LC-MS) analysis could observe a total of 3,5006 peptide were identified, 5160.0 proteins identified, of which 4094.0 can be quantified. Differentially expressed proteins with significance were extracted by data analysis and subject to Gene Ontology (GO) analysis showed the overviews of dysregulated proteins in the biological process (BP), cell component (CC), and molecular function (MF) categories. Proteins related to brain repair was found to change significantly, including BDNF, IL-10, IL-6, TGF-β，partly demonstrating the effects of ZRS on improving the tissue injury.Conclusion: ZRS was found to contribute to brain repair caused by ZRS treatment, and this could be partly mediated through the anti-inflammation effect by upregulating the vitamin transport. The results confirmed that ZRS presented a unique protein profile that indicated the adaptive mechanisms in acute stroke.

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“…Among them, E2 is the most abundant estrogen with the strongest activity, which has significant neuroprotective effects on different kinds of brain injury [ 42 ]. Studies have shown that E2 plays a neuroprotective role in ischemic neuron injury by inhibiting the expression and function of acid-sensitive ion channel proteins in an ischemic stroke model [ 43 ]. In addition, intranasal administration of E2-loaded gelatin nanoparticles effectively improved cerebral ischemic injury in mice and provided neuroprotection [ 44 ].…”

Section: Introductionmentioning

confidence: 99%

Neuroprotective Mechanism of Icariin on Hypoxic Ischemic Brain Damage in Neonatal Mice

Wang

Yang

Zhou

et al. 2022

Oxidative Medicine and Cellular Longevity

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Objective. Our previous results showed that icariin (ICA) could inhibit apoptosis and provide neuroprotection against hypoxic-ischemic brain damage (HIBD) in neonatal mice, but the specific mechanism of its neuroprotective effect remains unknown. This study aims at exploring whether ICA plays a neuroprotective role in apoptosis inhibition by regulating autophagy through the estrogen receptor α (ERα)/estrogen receptor β (ERβ) pathway in neonatal mice with HIBD. Methods. A neonatal mouse model of HIBD was constructed in vivo, and an oxygen and glucose deprivation (OGD) model in HT22 cells from the hippocampal neuronal system was constructed in vitro. The effects of ICA pretreatment on autophagy and the expression of ERα and ERβ were detected in vitro and in vivo, respectively. ICA pretreatment was also supplemented with the autophagy inhibitor 3-methyladenine (3-MA), ERα inhibitor methylpiperidino pyrazole (MPP), and ERβ inhibitor 4-(2-phenyl-5,7-bis (trifluoromethyl) pyrazolo [1,5-a] pyramidin-3-yl) phenol (PHTPP) to further detect whether ICA pretreatment can activate the ERα/ERβ pathway to promote autophagy and reduce HIBD-induced apoptosis to play a neuroprotective role against HIBD in neonatal mice. Results. ICA pretreatment significantly promoted autophagy in HIBD mice. Treatment with 3-MA significantly inhibited the increase in autophagy induced by ICA pretreatment, reversed the neuroprotective effect of ICA pretreatment, and promoted apoptosis. Moreover, ICA pretreatment significantly increased the expression levels of the ERα and ERβ proteins in HIBD newborn mice. Both MPP and PHTPP administration significantly inhibited the expression levels of the ERα and ERβ proteins activated by ICA pretreatment, reversed the neuroprotective effects of ICA pretreatment, inhibited the increase in autophagy induced by ICA pretreatment, and promoted apoptosis. Conclusion. ICA pretreatment may promote autophagy by activating the ERα and ERβ pathways, thus reducing the apoptosis induced by HIBD and exerting a neuroprotective effect on neonatal mice with HIBD.

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Neurosteroids and their receptors in ischemic stroke: From molecular mechanisms to therapeutic opportunities

Cited by 26 publications

References 340 publications

The proteomics analysis of the effects of Zhishi Rhubarb soup on ischaemic stroke

The proteomics analysis of the effects of Zhishi Rhubarb soup on ischaemic stroke

The Proteomics Analysis of the Effects of Zhishi Rhubarb Soup on Ischemic Stroke

Neuroprotective Mechanism of Icariin on Hypoxic Ischemic Brain Damage in Neonatal Mice

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