Neurotoxic Effects of Gestational Administration of Low-dose Lead Acetate

Antonio, M.T.; Martínez, Sara Pérez; Leret, M.L.; Corpas, Isabel

doi:10.1002/(sici)1099-1263(199609)16:5<431::aid-jat372>3.0.co;2-h

Cited by 12 publications

(2 citation statements)

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“…Despite the use of gestational, newborn, and adult rats as valid model systems for studying the neurotoxic effects of cadmium [104][105][106][107][108][109], the possible alterations in EBA in the developing rat brain as a result of cadmium exposure have not been widely studied. Rodents, including rats and mice, have been extensively used in studies to understand the pathophysiologic mechanisms of environmentally induced CNS pathologies such as cadmium intoxication and other disorders in humans.…”

Section: Discussionmentioning

confidence: 99%

The Effect of Short-Term Exposure to Cadmium on the Expression of Vascular Endothelial Barrier Antigen in the Developing Rat Forebrain and Cerebellum: A Computerized Quantitative Immunofluorescent Study

Ibiwoye

Snyder

Lyons

et al. 2022

Cureus

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Clinical and laboratory studies have shown that environmental exposure to cadmium produces damage to several organs, including bones, lungs, and kidneys. The involvement of cadmium in central nervous system (CNS) disorders has also been widely reported, but the precise pathophysiological mechanism is not yet fully understood. Children who were exposed to cadmium during pregnancy are known to suffer from developmental delays, learning difficulties, attention deficit hyperactivity disorder (ADHD), and other cognitive and neurobehavioral deficits. Results from numerous studies suggest that dysfunction of the blood-brain barrier (BBB) structures is an important step in the neurotoxicity of cadmium. A rat-specific BBB marker protein, the endothelial barrier antigen (EBA), has been previously isolated and classified by Sternberger and others. The mouse IgG1 clone, anti-endothelial barrier antigen (anti-EBA), detects a protein triplet (23.5kDa, 25 kDa, and 30kDa) localized to the luminal surface of central and peripheral nervous system (CNS and PNS) vascular endothelial cells with selective permeability barrier functions. This marker has been widely used for characterizing BBB alterations under demyelinating, inflammatory, and other CNS pathologies. Many studies have been published using the rat model system for studying the neurotoxic effect of acute and chronic exposure to cadmium.We applied the indirect immunofluorescent techniques using the anti-EBA antibody in conjunction with the Olympus cellSens computerized image analysis to detect and quantify the surface areas of BBB-competent microvessel profiles in paraformaldehyde-fixed, paraffin-embedded brains of term-delivered young rats after intraperitoneal injection of a single dose of cadmium chloride. We detected a statistically significant reduction in EBA-positive microvessel surface areas in the forebrain (t = 5.86, df = 1789, p-value < 0.001) and cerebellum (t=73.40, df=1337, p < 0.001) of cadmium-treated rats compared to the normal controls. Thus, this study supports the hypothesis that the EBA is a sensitive and measurable indicator for quantitative assessment of the impact of cadmium exposure in the developing rat brain.

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Section: Discussionmentioning

confidence: 99%

The Effect of Short-Term Exposure to Cadmium on the Expression of Vascular Endothelial Barrier Antigen in the Developing Rat Forebrain and Cerebellum: A Computerized Quantitative Immunofluorescent Study

Ibiwoye

Snyder

Lyons

et al. 2022

Cureus

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“…The amounts of DNA and RNA in brain were measured by the method of Schneider 9 using a UV spectrometer (Pharmacia LK B-ultrospect III). 10 Proteins were measured by Lowry's method 11 and total lipid content by a constant weight method. 12 Additional encephalons from each group were dissected 13 at autopsy to separate the mesencephalon, metencephalon, diencephalon and telencephalon.…”

Section: Methodsmentioning

confidence: 99%

Gestational administration of cadmium alters the neurotransmitter levels in newborn rat brains

et al. 1998

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The effects of gestational and early lactational intoxication by cadmium (Cd) were studied in the brain of young Wistar rats. Pregnant rats were exposed to 10 mg of cadmium acetate per litre of drinking water, from initiation of pregnancy to parturition or until postnatal day 5. At birth or on postnatal day (PND) 5 the pups were weighed, sacrificed and brains were removed and frozen for later study. Protein, lipid and nucleic acid contents were measured and the brain Cd concentration was determined. Levels of dopamine (DA), 5-hydroxytryptamine (5-HT) and their respective metabolites 3,4-dihydroxyphenylacetic acid (DOPAC) and 5-hydroxyindoleacetic acid (5-HIAA) were measured in neonatal pup brain by higher performance liquid chromatography with electrochemical detection. The results from this experiment showed that Cd increased the 5-HT and 5-HIAA contents in all areas of the brain and the DA and DOPAC levels in mesencephalon, but decreased the DA and DOPAC levels in the metencephalon. On the other hand, Cd intoxication did not modify the other biochemical parameters measured, with the exception of a decrease in nucleic acids on PND 5.

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Anxiety and memory deficits induced by tannery effluent in C57BL/6J female mice

Guimarães

Ferreira

Souza

et al. 2016

Environ Sci Pollut Res

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This study aimed to evaluate the behavior of female C57Bl/6J mice exposed to tannery effluents diluted in drinking water. Female mice were divided into a control group, in which the animals received only drinking water, and experimental groups, which received raw tannery effluent in 7.5 and 15 % concentrations diluted in water (period of 60 days). In the last experimental week, the mice (in diestrus phase) were subjected to different behavioral tests: elevated plus-maze, open-field test, forced swim test, and object recognition test. Our data demonstrated that exposure to tannery effluent increased the anxiety index of animals and decreased the locomotion ratio in the central quadrants/total, indicating an increase in anxiety-like behavior. Regarding the forced swim test, we did not observe changes in the evaluated behaviors. There were no statistically significant differences in the recognition index of the novel and familiar object in the groups exposed to tannery effluent compared with the control group, indicating a possible influence of the constituents of tannery effluent on cognition. Thus, our findings support the hypothesis that effluents, containing neurotoxic substances, could cause behavioral disruptions in female C57Bl/6J mice.

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Neurotoxic Effects of Gestational Administration of Low-dose Lead Acetate

Cited by 12 publications

References 1 publication

The Effect of Short-Term Exposure to Cadmium on the Expression of Vascular Endothelial Barrier Antigen in the Developing Rat Forebrain and Cerebellum: A Computerized Quantitative Immunofluorescent Study

The Effect of Short-Term Exposure to Cadmium on the Expression of Vascular Endothelial Barrier Antigen in the Developing Rat Forebrain and Cerebellum: A Computerized Quantitative Immunofluorescent Study

Gestational administration of cadmium alters the neurotransmitter levels in newborn rat brains

Anxiety and memory deficits induced by tannery effluent in C57BL/6J female mice

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