Neurotoxicity evaluation of three root canal sealers on cultured rat trigeminal ganglion neurons

Abstract

BackgroundThe aim of this study was to investigate the possible neurotoxic effects of 3 root canal sealers (RCSs) (AH Plus, GuttaFlow, iRoot SP) on cultured rat trigeminal ganglion (TG) neurons.Material and MethodsPrimary cultures of TG neurons were obtained from 1 to 2-day old rats. Freshly mixed RCSs were incubated in sterile phosphate buffered saline and cells were incubated with supernatants of the RCSs for different time intervals (1-, 3-, 6- and 24-h; 1 or 1/10 diluted) and viability/cytotoxicity was tested by counting the number of live cells. Pair of dishes with cells from the same culture incubated with only culture medium was considered as negative controls. Cell images were captured and acquired at x200 magnification using a microscope equipped with a camera using special image program. The viable cells were manually counted assigned from the images for each dose and incubation duration. Data was analysed by using 1-way analysis of variance with Tukey post hoc tests.ResultsThere was no significant change in cell viability after short duration of incubation (1- and 3-h) with the supernatant of any of RCSs, except for undiluted-AH Plus at 3-h. When AH Plus was compared with other RCSs, for diluted supernatants, there was only significant difference between iRoot SP and AH Plus at 24-h (P<0.05). Whereas undiluted-AH Plus was significantly more cytotoxic for 3-, 6- and 24-h periods as compared to respective incubation periods of undiluted other groups (P<0.05). GuttaFlow groups had similar neurotoxic effect on cells for all test periods.ConclusionsAll tested RCSs exhibited a variable degree of neurotoxicity on these primary sensory neurons of orofacial tissues, depending on their chemical compositions. GuttaFlow and iRoot SP evoked a less toxic response to TG cells than AH Plus. Key words:Neurotoxicity, trigeminal ganglia, cell culture, root canal sealer, AH Plus, GuttaFlow, iRoot SP.

Postoperative pain after root canal filling with different endodontic sealers: a randomized clinical trial

“…8,9 Therefore, it can be expected that root canal sealers may stimulate an inflammatory response and activate sensory neurons. 10,11,12 Consequently, root canal sealers may be related to postoperative pain after root canal treatment. Ruparel et al 10 suggested that root canal sealers might activate trigeminal nociceptors in vitro, and that this activation, along with the immunologic response, may trigger pain symptoms and cause flare-ups.…”

Clinical outcome of bioceramic sealer iRoot SP extrusion in root canal treatment: a retrospective analysis

“…Its favorable properties might be one of the critical reasons. iRoot SP has been reported to be less toxic [10][11][12][13][14][15][16][17] than AH Plus and MTA and to induce a relatively milder inflammatory response [18]. Furthermore, the excellent apical sealing ability of iRoot SP also accounts for the favorable outcome, which results in less apical microleakage of bacteria [19][20][21][22][23][24][25].…”

“…It attracts clinical operators' attention due to its superior properties. Specifically, iRoot SP is more biocompatible than AH Plus and MTA [10][11][12][13][14][15][16][17] and induces a mild inflammatory response in mouse subcutaneous tissue [18]. In addition, iRoot SP possesses an excellent apical sealing ability, which results in less apical microleakage [19][20][21][22][23][24][25].…”

Proses penyembuhan lesi periapikal pada radiografi periapikal menggunakan Software Image J

“…Guttaflow dan Endomethasone memiliki efek toksik yang lebih rendah dibandingkan dengan AH Plus. 10 Hasil dari penelitian yang terlihat pada tabel 3 terdapat beberapa waktu kontrol yaitu 1-2 minggu, 2-4 minggu, dan 5-8 minggu pada perawatan endodontik setelah pengisian saluran akar. Waktu kontrol setelah 1-2 minggu menunjukkan pengurangan luas lesi sebesar 10,03±5,38 mm2 dan peningkatan densitas sebesar 8,47±6,26%.…”