Neurotoxins in the venom gland of Calommata signata, a burrowing spider

Han, Qianqian; Huang, Lixin; Li, Jingjing; Wang, Zhaoying; Gao, Haoli; Yang, Zhiming; Zhou, Zhangjin; Liu, Zewen

doi:10.1016/j.cbd.2021.100871

Cited by 3 publications

(2 citation statements)

References 67 publications

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“…Total extracted RNA was then quality-checked and quantified. cDNA libraries were constructed and sequenced following procedures described by Han et al and Liu et al [15-16]. The transcriptome datasets are available in the NCBI database under the accession PRJNA757917.…”

Section: Methodsmentioning

confidence: 99%

Chitosan oligosaccharide induces plant resistance gene expression inPinus massoniana

Yin

Guo

Fang

et al. 2023

Preprint

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Chitosan oligosaccharides are the main degradation products from chitosan or chitin and have been reported to induce resistance to diseases in herbaceous plants like cucumber and Arabidopsis. Concomitantly, pine wilt disease is a devastating disease of conifer tree species. Here, we hypothesized that chitosan oligosaccharides induce plant resistance gene (PRG) expression in the woody plant Masson pine, Pinus massoniana. Chitosan oligosaccharides were inoculated into P. massoniana seedlings and the BGISEQ-500 platform was used to generate transcriptomes from chitosan oligosaccharide-treated P. massoniana and control seedlings. A total of 501 differentially expressed genes (DEGs) were identified by comparing the treatment and control groups. A total of 251 (50.1%) DEGs were up-regulated in the treatment relative to the control seedlings and 250 (49.9%) were down-regulated. Inoculation of chitosan oligosaccharide induced the expression of 31 PRGs in P. massoniana seedlings and the relative expression levels of six of the PRGs were verified by RT-qPCR. This is the first study to demonstrate that chitosan oligosaccharide induces the expression of PRGs in a tree species. These results provide important insights into the function of chitosan oligosaccharides and further the prospects of developing a chitosan oligosaccharide-based immune inducer for controlling pine wilt disease.

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Section: Methodsmentioning

confidence: 99%

Chitosan oligosaccharide induces plant resistance gene expression inPinus massoniana

Yin

Guo

Fang

et al. 2023

Preprint

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“…We previously reported the neurotoxins from the venom gland of a burrowing spider Calommata signata. 19 In the present study, we obtained the recombinant neurotoxin U1-Atypitoxin-Cs1a from the same spider, characterized its molecular targets and evaluated its insecticidal activity against three insect pests of agricultural importance: the rice planthopper Nilaparvata lugens, the fall armyworm Spodoptera frugiperda and the diamondback moth Plutella xylostella.…”

Section: Introductionmentioning

confidence: 99%

Insecticidal toxicity of ω‐Atypitoxin‐Cs1a and its inhibitory effects on insect voltage‐gated calcium channels

Yan

Han

et al. 2023

Pest Management Science

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BACKGROUNDExcessive use of chemical insecticides raises concerns about insecticide resistance, urging the development of novel insecticides. Peptide neurotoxins from spider venom are an incredibly rich source of ion channel modulators with potent insecticidal activity. A neurotoxin U1‐Atypitoxin‐Cs1a from the spider Calommata signata was annotated previously. It was of interest to investigate its insecticidal activity and potential molecular targets.RESULTSCs1a was heterologously expressed, purified and pharmacologically characterized here. The recombinant neurotoxin inhibited high‐voltage‐activated calcium channel currents with an median inhibitory concentration (IC50) value of 0.182 ± 0.026 μm on cockroach DUM neurons and thus was designated as ω‐Atypitoxin‐Cs1a. The recombinant Cs1a was toxic to three insect pests of agricultural importance, Nilaparvata lugens, Spodoptera frugiperda and Plutella xylostella with median lethal concentration (LD50) values of 0.121, 0.172 and 0.356 nmol g−1, respectively, at 24 h postinjection. Cs1a was equivalently toxic to both insecticide‐susceptible and ‐resistant insects. Cs1a exhibited low toxicity to Danio rerio with an LD50 of 2.316 nmol g−1.CONCLUSIONOur results suggest that ω‐Atypitoxin‐Cs1a is a potent CaV channel inhibitor and an attractive candidate reagent for pest control and resistance management. © 2023 Society of Chemical Industry.

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Unveiling Diversity and Function: Venom-Associated Microbes in Two Spiders, Heteropoda venatoria and Chilobrachys guangxiensis

Zhao,

Zhang,

et al. 2024

Microb Ecol

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Neurotoxins in the venom gland of Calommata signata, a burrowing spider

Cited by 3 publications

References 67 publications

Chitosan oligosaccharide induces plant resistance gene expression inPinus massoniana

Chitosan oligosaccharide induces plant resistance gene expression inPinus massoniana

Insecticidal toxicity of ω‐Atypitoxin‐Cs1a and its inhibitory effects on insect voltage‐gated calcium channels

Unveiling Diversity and Function: Venom-Associated Microbes in Two Spiders, Heteropoda venatoria and Chilobrachys guangxiensis

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