Neurotransmitter Switching Coupled to β-Adrenergic Signaling in Sympathetic Neurons in Prehypertensive States

Bardsley, Emma N.; Davis, Harvey; Buckler, Keith J.; Paterson, David J.

doi:10.1161/hypertensionaha.118.10844

Cited by 32 publications

(25 citation statements)

References 95 publications

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“…expression in the SHR strain at four and 16-weeks of age relative to controls. These data suggest that perturbations in AngII synthesis may occur alongside sympathetic dysfunction in this strain, where characteristics of sympathetic dysfunction include elevated intracellular Ca 2+ [53,54], increased neuronal firing frequency [55], enhanced noradrenaline release [56,57] and alterations in neurotransmitter profiles [40,58]. We identified the presence of AngII and Ang1-7 receptors on sympathetic stellate ganglia of human and rat.…”

Section: Discussionmentioning

confidence: 88%

“…Rats were anaesthetized in an induction chamber (3-5% isoflurane) and humanely killed by a Home Office approved Schedule 1 method: overdose of pentobarbital (Euthatal, 200 mg/ml) and exsanguination. Dissection was carried out as previously described [40].…”

Section: Rat Sympathetic Cardiac Ganglia Dissectionmentioning

confidence: 99%

“…For rat ganglia, the SuperScript™ III VILO™ cDNA synthesis protocol was followed according to manufacturer's instructions (ThermoFisher). For human ganglia, the SuperScript™ IV VILO™ cDNA synthesis protocol was followed according to manufacturer's instructions (ThermoFisher) as previously described [40]. Concentrations of cDNA in each sample and the 260/280 ratios were calculated (Nano-Drop Lite) to detect the presence of contaminants.…”

Section: 7mentioning

confidence: 99%

“…For FRET measurements of cytosolic cGMP, sympathetic stellate neurons from four-week-old preSHR Wistar rats were cultured into a single-cell suspension using a previously described method [40] and transduced with the FRET biosensor cGi500 (3.42 × 10 8 pfu/well, Vector BioLabs) in neuronal plating media. After 24-h, the virus-containing medium was replaced with virus-free neuronal plating medium and the neurons were incubated for a further 24-36 h (37°C, 5% CO 2 ) to obtain an appropriate level of biosensor expression for FRET imaging as previously described [40,47].…”

Section: Förster Resonance Energy Transfer (Fret)mentioning

confidence: 99%

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Angiotensin peptide synthesis and cyclic nucleotide modulation in sympathetic stellate ganglia

Bardsley

Neely

Paterson

2020

Journal of Molecular and Cellular Cardiology

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Chronically elevated angiotensin II is a widely-established contributor to hypertension and heart failure via its action on the kidneys and vasculature. It also augments the activity of peripheral sympathetic nerves through activation of presynaptic angiotensin II receptors, thus contributing to sympathetic over-activity. Although some cells can synthesise angiotensin II locally, it is not known if this machinery is present in neurons closely coupled to the heart. Using a combination of RNA sequencing and quantitative real-time polymerase chain reaction, we demonstrate evidence for a renin-angiotensin synthesis pathway within human and rat sympathetic stellate ganglia, where significant alterations were observed in the spontaneously hypertensive rat stellate ganglia compared with Wistar stellates. We also used Förster Resonance Energy Transfer to demonstrate that administration of angiotensin II and angiotensin 1-7 peptides significantly elevate cyclic guanosine monophosphate in the rat stellate ganglia. Whether the release of angiotensin peptides from the sympathetic stellate ganglia alters neurotransmission and/or exacerbates cardiac dysfunction in states associated with sympathetic over activity remains to be established.

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Section: Discussionmentioning

confidence: 88%

Section: Rat Sympathetic Cardiac Ganglia Dissectionmentioning

confidence: 99%

Section: 7mentioning

confidence: 99%

Section: Förster Resonance Energy Transfer (Fret)mentioning

confidence: 99%

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Angiotensin peptide synthesis and cyclic nucleotide modulation in sympathetic stellate ganglia

Bardsley

Neely

Paterson

2020

Journal of Molecular and Cellular Cardiology

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“…Within this framework, activation of β 1 - and β 2 -adrenoceptor subtypes has been shown to promote sympathetic stimulation ( i.e. , an increase in noradrenaline release) via a cAMP-PKA dependent pathway [ 93 ] (Table 2 ). Furthermore, β-adrenoceptors are expressed in smooth muscle cells of some vascular beds ( e.g.…”

Section: Monoaminergic Receptors Modulating the Sympathetic Perivascumentioning

confidence: 99%

Monoaminergic Receptors as Modulators of the Perivascular Sympathetic and Sensory CGRPergic Outflows

Marichal‐Cancino

González‐Hernández

Muñoz-Islas

et al. 2020

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: Blood pressure is a highly controlled cardiovascular parameter that normally guarantees an adequate blood supply to all body tissues. This parameter is mainly regulated by vascular peripheral resistance and is maintained by local mediators (i.e., autacoids), and by the nervous and endocrine systems. Regarding the nervous system, blood pressure can be modulated at central level by regulating the autonomic output. However, at peripheral level there exists a modulation by activation of prejunctional monoaminergic receptors in autonomic- or sensory-perivascular fibers. These modulatory mechanisms on resistance blood vessels exert an effect on the release of neuroactive substances from the autonomic or sensory fibers that modify blood pressure. Certainly, resistance blood vessels are innervated by perivascular: (i) autonomic sympathetic fibers (producing vasoconstriction mainly by noradrenaline release); and (ii) peptidergic sensory fibers [producing vasodilatation mainly by calcitonin gene-related peptide (CGRP) release]. In the last years, by using pithed rats, several monoaminergic mechanisms for controlling both the sympathetic and sensory perivascular outflows have been elucidated. Additionally, several studies have shown the functions of many monoaminergic auto-receptors and hetero-receptors expressed on perivascular fibers that modulate neurotransmitter release. On this basis, the present review: (i) summarizes the modulation of the peripheral vascular tone by adrenergic, serotoninergic, dopaminergic, and histaminergic receptors on perivascular autonomic (sympathetic) and sensory fibers, and (ii) highlights that these monoaminergic receptors are potential therapeutic targets for the development of novel medications to treat cardiovascular diseases (with some of them explored in clinical trials or already in clinical use).

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Stellate ganglionitis in sudden cardiac death: A case report

Duffy¹,

Garland

Ondruschka

et al. 2021

Autonomic Neuroscience

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Neurotransmitter Switching Coupled to β-Adrenergic Signaling in Sympathetic Neurons in Prehypertensive States

Abstract: Supplemental Digital Content is available in the text.

Cited by 32 publications

References 95 publications

Angiotensin peptide synthesis and cyclic nucleotide modulation in sympathetic stellate ganglia

Angiotensin peptide synthesis and cyclic nucleotide modulation in sympathetic stellate ganglia

Monoaminergic Receptors as Modulators of the Perivascular Sympathetic and Sensory CGRPergic Outflows

Stellate ganglionitis in sudden cardiac death: A case report

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