Neurotrophic factors loaded TGN-modified chitosan nanoparticles ameliorate symptoms of MS through GATA3/FOXP3 and targeting Th1/2 cells pathways

Khodakarimi, Sina; Zarebkohan, Amir; Mohaddes, Gisou; Shiri-Shahsavari, Mohammad Reza; Omrani, Mohammad Hassan; Sepasi, Tina; Beyrampour-Basmenj, Hanieh; Ebrahimi-Kalan, Abbas

doi:10.1016/j.jddst.2023.104570

Cited by 1 publication

(2 citation statements)

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“…BMSCs were identified by flow cytometry 30 . For this purpose, the cells were separated from the bottom of the dish using trypsin in the third passage, then rinsed with PBS and permeabilized using a 1% Triton solution.…”

Section: Methodsmentioning

confidence: 99%

“…BMSCs were identified by flow cytometry. 30 For this purpose, the cells were separated from the bottom of the dish using trypsin in the third passage, then rinsed with PBS and permeabilized using a 1% Triton solution. Following multiple washes with PBS, the cells were subjected to the primary antibody under dark conditions at…”

Section: Flow Cytometry To Identify Bmscsmentioning

confidence: 99%

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Mesenchymal stem cell‐derived exosomes improve neurogenesis and cognitive function of mice with methamphetamine addiction: A novel treatment approach

Fallahi,

Zangbar,

Farajdokht

et al. 2024

CNS Neurosci Ther

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BackgroundMethamphetamine (METH) is a psychostimulant substance with highly addictive and neurotoxic effects, but no ideal treatment option exists to improve METH‐induced neurocognitive deficits. Recently, mesenchymal stem cells (MSCs)‐derived exosomes have raised many hopes for treating neurodegenerative sequela of brain disorders. This study aimed to determine the therapeutic potential of MSCs‐derived exosomes on cognitive function and neurogenesis of METH‐addicted rodents.MethodsMale BALB/c mice were subjected to chronic METH addiction, followed by intravenous administration of bone marrow MSCs‐derived exosomes. Then, the spatial memory and recognition memory of animals were assessed by the Barnes maze and the novel object recognition test (NORT). The neurogenesis‐related factors, including NeuN and DCX, and the expression of Iba‐1, a microglial activation marker, were assessed in the hippocampus by immunofluorescence staining. Also, the expression of inflammatory cytokines, including TNF‐α and NF‐κB, were evaluated by western blotting.ResultsThe results showed that BMSCs‐exosomes improved the time spent in the target quadrant and correct‐to‐wrong relative time in the Barnes maze. Also, NORT's discrimination index (DI) and recognition index (RI) were improved following exosome therapy. Additionally, exosome therapy significantly increased the expression of NeuN and DCX in the hippocampus while decreasing the expression of inflammatory cytokines, including TNF‐α and NF‐κB. Besides, BMSC‐exosomes down‐regulated the expression of Iba‐1.ConclusionOur findings indicate that BMSC‐exosomes mitigated METH‐caused cognitive dysfunction by improving neurogenesis and inhibiting neuroinflammation in the hippocampus.

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Section: Methodsmentioning

confidence: 99%

Section: Flow Cytometry To Identify Bmscsmentioning

confidence: 99%