Neurotrophin gene expression in rat brain under the action of Semax, an analogue of ACTH4–10

Agapova, T. Yu.; Agniullin, Ya. V.; Шадрина, М. И.; Shram, S. I.; Slominsky, Petr; Lymborska, S.A.; Myasoedov, N. F.

doi:10.1016/j.neulet.2007.02.042

Cited by 15 publications

(9 citation statements)

References 21 publications

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“…Previously, Dolotov et al (2006) did not reveal any influence of Semax on Bdnf transcription in the cortex of intact rats, and the decrease in Ngf transcription was registered by Agapova et al (2007). Indeed, despite the different type of infusion and time points used in our study, similar features were observed as a result of the influence of Semax: in the cortex of rats without surgery Semax did not show any effect on the expression of Bdnf and Ngf and their receptors and inhibited the transcription of Nt-3.…”

Section: Neurotrophin Receptor Mrna Expression In the Cortex Of Rats mentioning

confidence: 93%

“…It has been suggested that the neuroprotective effects of Semax are related to neurotrophin gene expression: the rapid induction of Bdnf and Ngf mRNAs in rat glial cell cultures has been observed after Semax treatment (Shadrina et al 2001). Subsequently, it was demonstrated in vivo that Semax application increases both protein and mRNA levels of Bdnf, and TrkB, and mRNA level of Ngf in rat hippocampus (Dolotov et al 2006;Agapova et al 2007). At the same time, the studies of Semax degradation in rat serum and its entry to the rat brain (Potaman et al 1991(Potaman et al , 1993Dolotov et al 2004) and the studies of self-maintained effects of PGP and other proline-containing peptides (Lyapina et al 2000;Samonina et al 2001;Zhuikova et al 2003;Storozhevykh et al 2007) raise the question of whether C-terminal PGP tripeptide contributes to the effects of Semax.…”

Section: Introductionmentioning

confidence: 99%

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Semax and Pro-Gly-Pro Activate the Transcription of Neurotrophins and Their Receptor Genes after Cerebral Ischemia

Дмитриева

Povarova²,

Skvortsova³

et al. 2009

Cell Mol Neurobiol

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Consisting of a fragment of ACTH(4-7) and C-terminal PGP tripeptide, the polypeptide Semax is successfully used for acute stroke therapy. Previous experiments showed rapid induction of Bdnf, Ngf, and TrkB expression in intact rat hippocampus following Semax treatment. To investigate the mRNA expression of neurotrophins and their receptors after treatment with either Semax or PGP, the rat brains were analyzed at three time points following a permanent middle cerebral artery occlusion (pMCAO). We have shown for the first time that both Semax and PGP activate the transcription of neurotrophins and their receptors in the cortex of rats subjected to pMCAO. The profiles of transcription alteration under PGP and Semax treatment were partially overlapped. Semax enhanced the transcription of Bdnf, TrkC, and TrkA 3 h after occlusion, Nt-3 and Ngf 24 h after occlusion, and Ngf 72 h after occlusion. PGP enhanced the transcription of Bdnf and TrkC 3 h after pMCAO and Ngf, TrkB, TrkC, and TrkA 24 h after pMCAO. The analysis of the transcription alterations under PGP and Semax treatment in the cortex of rats without surgery, sham-operated rats and rats subjected to pMCAO revealed that Semax selectively affected the transcription of neurotrophins and their receptors in the ischemic rat cortex, whereas the influence of PGP was mainly unspecific.

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Section: Neurotrophin Receptor Mrna Expression In the Cortex Of Rats mentioning

confidence: 93%

Section: Introductionmentioning

confidence: 99%

Semax and Pro-Gly-Pro Activate the Transcription of Neurotrophins and Their Receptor Genes after Cerebral Ischemia

Дмитриева

Povarova²,

Skvortsova³

et al. 2009

Cell Mol Neurobiol

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“…Our recent findings showed that a single intranasal application of Semax increased the rat hippocampal expression of BDNF and NGF within 1 h and upregulated the expression of the BDNF gene in the brainstem and cerebellum. In contrast, NGF gene expression decreased in the rat frontal cortex (Agapova et al 2007). In connection with all these findings, the aim of the present work was to analyze the temporary dynamics of NGF and BDNF expression under Semax action in rat hippocampus, frontal cortex, and retina.…”

Section: Introductionmentioning

confidence: 94%

“…The relative levels of NGF, BDNF, and Rpl3 cDNAs were analyzed by SYBR Green real-time polymerase chain reaction (PCR) using primers described previously (Agapova et al 2007). The reactions were carried out using a SYBR Green I Real-Time PCR Kit (Syntol, Russia).…”

Section: Real-time Quantitative Reverse Transcription-pcrmentioning

confidence: 99%

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Comparison of the Temporary Dynamics of NGF and BDNF Gene Expression in Rat Hippocampus, Frontal Cortex, and Retina Under Semax Action

et al. 2009

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Neurotrophins are a family of structurally related proteins that regulate the survival, differentiation, and maintenance of function of different neuron populations. Some peptides are able to affect the production and activity of neurotrophins. One of these synthetic peptides is heptapeptide Semax, an analog of the N-terminal adrenocorticotropic hormone fragment 4-10. It is known that Semax has effects on learning and memory formation and exerts some neuroprotective effects in rodents and humans. Male Wistar rats were treated for 20 min, 40 min, 90 min, 3 h, 8 h, and 24 h with Semax. Nerve growth factor (NGF) and brain-derived neurotrophic factor (BDNF) gene expression in rat brain and retina was analyzed by real-time polymerase chain reaction. It was revealed that after Semax administration the multidirectional activation of the expression of the genes under investigation in the hippocampus, frontal cortex, and retina was observed. The expression of both neurotrophin genes was decreased in rat hippocampus and retina 20 min after Semax administration and was increased in the frontal cortex. The expression levels of NGF remained practically constant in the retina at the initial stage, whereas the expression levels of BDNF were significantly increased 90 min after Semax administration.

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Pharmacotranscriptomics of peptide drugs with neuroprotective properties

Dergunova

Filippenkov

Limborska

et al. 2020

Medicinal Research Reviews

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Here we present a review of studies on the effects of peptides with neuroprotective properties on gene transcription in nerve cells. The few published works in this area clearly demonstrate massive changes in cell transcriptomes induced by peptides under normal conditions and under conditions of experimental brain ischemia. These changes significantly affect signaling and metabolic pathways, affecting various body systems and confirming the multiple target actions of peptides. The importance of noncoding RNAs in the regulation of these processes is shown, and we discuss the prospects of research for determining the main mechanisms of peptide regulation, which is necessary for the further development of drugs with targeted neuroprotective effects.

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Neurotrophin gene expression in rat brain under the action of Semax, an analogue of ACTH4–10

Cited by 15 publications

References 21 publications

Semax and Pro-Gly-Pro Activate the Transcription of Neurotrophins and Their Receptor Genes after Cerebral Ischemia

Semax and Pro-Gly-Pro Activate the Transcription of Neurotrophins and Their Receptor Genes after Cerebral Ischemia

Comparison of the Temporary Dynamics of NGF and BDNF Gene Expression in Rat Hippocampus, Frontal Cortex, and Retina Under Semax Action

Pharmacotranscriptomics of peptide drugs with neuroprotective properties

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