Neurotrophins and neurotrophin receptors in some neural crest‐derived tumours (ganglioneuroma, phaeochromocytoma and paraganglioma)

Pello,; Guate,; Escaf,; Vega, J.

doi:10.1046/j.1365-2559.1999.00605.x

Cited by 9 publications

(3 citation statements)

References 60 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…54,55 RT-PCR results and results from immunohistochemistry (also at lower antibody dilutions), however, suggest additional expression sites. Low titers of BDNF, NT-4/5, and Trk-A mRNA transcripts and high titers of NT-3 transcripts were found in rat hepatocytes (Fig.…”

Section: Discussionmentioning

confidence: 91%

Human and Rat Hepatic Stellate Cells Express Neurotrophins and Neurotrophin Receptors

Cassiman¹,

Denef²,

et al. 2001

View full text Add to dashboard Cite

Section: Discussionmentioning

confidence: 91%

Human and Rat Hepatic Stellate Cells Express Neurotrophins and Neurotrophin Receptors

Cassiman¹,

Denef²,

et al. 2001

View full text Add to dashboard Cite

“…Pello et al [ 30 ] were unable to demonstrate the NT-3 presence on the CB of adult human subjects by immunohistochemistry. The immunopositivity for NT-3 was later demonstrated by Atanasova and Lazarov [ 20 ] in rat CB ( Table 1 ).…”

Section: Nerve Growth Factor Familymentioning

confidence: 99%

Growth Factors in the Carotid Body—An Update

Stocco

Barbon

Tortorella

et al. 2020

IJMS

View full text Add to dashboard Cite

The carotid body may undergo plasticity changes during development/ageing and in response to environmental (hypoxia and hyperoxia), metabolic, and inflammatory stimuli. The different cell types of the carotid body express a wide series of growth factors and corresponding receptors, which play a role in the modulation of carotid body function and plasticity. In particular, type I cells express nerve growth factor, brain-derived neurotrophic factor, neurotrophin 3, glial cell line-derived neurotrophic factor, ciliary neurotrophic factor, insulin-like-growth factor-I and -II, basic fibroblast growth factor, epidermal growth factor, transforming growth factor-α and -β, interleukin-1β and -6, tumor necrosis factor-α, vascular endothelial growth factor, and endothelin-1. Many specific growth factor receptors have been identified in type I cells, indicating autocrine/paracrine effects. Type II cells may also produce growth factors and express corresponding receptors. Future research will have to consider growth factors in further experimental models of cardiovascular, metabolic, and inflammatory diseases and in human (normal and pathologic) samples. From a methodological point of view, microarray and/or proteomic approaches would permit contemporary analyses of large groups of growth factors. The eventual identification of physical interactions between receptors of different growth factors and/or neuromodulators could also add insights regarding functional interactions between different trophic mechanisms.

show abstract

“…Even though NGF production has been observed in a variety of normal tissues (Santambrogio et al, 1994;Kokaia et al, 1993;Pincelli et al, 1997;Nilsson et al, 1997), very few investigators have found NET cells of neuroblastic phenotype that produce and secrete active NGF (for review see Vinores and Perez Polo, 1983). Even when NET cells seem to produce bioactive NGF, the levels are insufficient to activate the most sensitive receptors (Gill et al, 1998) or are completely undetectable (Pello et al, 1999). This is not surprising, insofar as NGF exerts a differentiating effect on immature neuroblasts, and production by tumor cells of neuroblastic origin would be disadvantageous for their growth.…”

mentioning

confidence: 99%

Retroviral transfer of the ?-nerve growth factor gene into murine neuroectodermal tumor cells modulates cell proliferation rate, neurite formation, and NGF binding site expression

Castellon

Mirkin²

2000

Journal of Neuroscience Research

View full text Add to dashboard Cite

The response of wild-type and genetically engineered neuroectodermal tumor (NET) cells to exogenous and endogenously synthesized nerve growth factor (NGF) was investigated. Differences in cell proliferation rate, neurite formation, and expression of NGF binding sites were quantitatively determined. Ecotropic retroviral vectors were used to transfer the genes for beta-galactosidase (beta-GAL) and NGF into wild-type C-1300 and Neuro-2A murine neuroblastoma (MNB) and rat pheochromocytoma (PC-12) cells. Conditioned media obtained from NET cells infected with the NGF gene contained biologically active NGF, whereas media from beta-GAL infected cells did not. Infection with the NGF vector induced a short-term decrease in cell proliferation rate and increased neurite formation in wild-type, substrate-adherent PC-12 and Neuro-2A MNB cells (P > 0.05). Incubation of wild-type C-1300, Neuro-2A MNB, and PC-12 cells with NGF (0-200 ng/ml) for 5 days significantly reduced proliferation rates in a concentration-dependent manner and increased neurite extrusion. All NGF-NET cells had a significantly diminished response to the antiproliferative action of exogenous NGF. Ligand binding assays with 125I-NGF demonstrated a marked reduction in the number of NGF binding sites on NGF-NET cells compared to wild type. The attenuated response of NGF-NET cells to exogenous NGF correlated positively with the down-regulation of NGF binding sites. In conclusion, beta-NGF gene transfer into wild-type NET cells induces the synthesis and secretion of NGF, temporarily decreases cell proliferation rate, increases neurite extrusion, down-regulates NGF binding sites, and reduces NET cell responsiveness to NGF. A putative role for NGF may be the modulation of NET cell proliferation and differentiation.

show abstract

Neurotrophins and neurotrophin receptors in some neural crest‐derived tumours (ganglioneuroma, phaeochromocytoma and paraganglioma)

Cited by 9 publications

References 60 publications

Human and Rat Hepatic Stellate Cells Express Neurotrophins and Neurotrophin Receptors

Human and Rat Hepatic Stellate Cells Express Neurotrophins and Neurotrophin Receptors

Growth Factors in the Carotid Body—An Update

Retroviral transfer of the ?-nerve growth factor gene into murine neuroectodermal tumor cells modulates cell proliferation rate, neurite formation, and NGF binding site expression

Contact Info

Product

Resources

About