Neurotrophins and their receptors in the tench retina during optic nerve regeneration

Caminos, Elena; Becker, Elena; Martı́n-Zanca, Dionisio; Vecino, Elena

doi:10.1002/(sici)1096-9861(19990215)404:3<321::aid-cne4>3.0.co;2-y

Cited by 55 publications

(31 citation statements)

References 72 publications

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“…Fish express the NTs commonly found in higher vertebrates (Hashimoto and Heinrich 1997;Hallböök et al 1998;2006) and other species-specific (Gotz and Schartl 1994;Lai et al 1998;Nilsson et al 1998;Caminos et al 1999). They also express Trk NT receptors (Martin et al 1995(Martin et al , 1998Caminos et al 1999;Heinrich and Lum 2000).…”

Section: Discussionmentioning

confidence: 99%

“…They also express Trk NT receptors (Martin et al 1995(Martin et al , 1998Caminos et al 1999;Heinrich and Lum 2000). Of particular interest for our research, BDNF and TrkB have been detected throughout zebrafish during embryonic development Lum et al 2001;Heinrich and Pagtakhan 2004) and also in neuromasts (Hashimoto and Heinrich 1997;Lum et al 2001).…”

Section: Discussionmentioning

confidence: 99%

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Expression of Brain-Derived Neurotrophic Factor and TrkB in the Lateral Line System of Zebrafish During Development

et al. 2010

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The neuromasts of the lateral line system are regarded as a model to study the mechanisms of hearing, deafness, and ototoxicity. The neurotrophins (NTs), especially brain-derived neurotrophic factor (BDNF), and its signaling receptor TrkB are involved in the development and maintenance of neuromasts. To know the period in which the BDNF/TrkB complex has more effects in the neuromast biology, the age-related changes were studied. Normal zebrafish from 10 to 180 days post-fertilization (dpf), as well as transgenic ET4 zebrafish 10 and 20 dpf, was analyzed using qRT-PCR, western blot, and immunohistochemistry. BDNF and TrkB mRNAs followed a parallel course, peaking at 20 dpf, and thereafter progressively decreased. Specific immunoreactivity for BDNF and TrkB was found co-localized in all hairy cells of neuromasts in 20 and 30 dpf; then, the number of immunoreactive cells decreased, and by 180 dpf BDNF remains restricted to a subpopulation of hairy cells, and TrkB to a few number of sensory and non-sensory cells. At all ages examined, TrkB immunoreactivity was detected in sensory ganglia innervating the neuromasts. The present results demonstrate that there is a parallel time-related decline in the expression of BDNF and TrkB in zebrafish. Also, the patterns of cell expression suggest that autocrine/paracrine mechanisms for this NT system might occur within the neuromasts. Because TrkB in lateral line ganglia did not vary with age, their neurons are potentially capable to respond to BDNF during the entire lifespan of zebrafish.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Expression of Brain-Derived Neurotrophic Factor and TrkB in the Lateral Line System of Zebrafish During Development

et al. 2010

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“…Neurotrophins also influence the central growth of connections from peripheral sensory receptors to the brain (Buckland and Cunningham, 1998;Caminos et al, 1999;Patel et al, 2003). Regarding a role for neurotrophins in peripheral innervation density by primary sensory nerves, BDNF-OE mice, in particular, exhibit greater than normal innervation of hair follicles.…”

Section: Neurotrophins and Innervation Densitymentioning

confidence: 99%

“…Gemmal and ganglion cell numbers are disrupted in the absence or overexpression of BDNF, or its receptor, tyrosine kinase B (TrkB) (Fritzsch et al, 1997;Nosrat et al, 1997;Cooper and Oakley, 1998;Oakley et al, 1998;Mistretta et al, 1999;Ringstedt et al, 1999;Krimm et al, 2001;Sun and Oakley, 2002). Thus, neurotrophins and their receptors influence, somehow, the development and maintenance of peripheral neural elements in taste as in other sensory systems (Schimmang et al, 1995;Fundin et al, 1997;Buckland and Cunningham, 1998;Caminos et al, 1999). Whether neurotrophins influence receptoneural connections has not been reported.…”

Section: Introductionmentioning

confidence: 99%

Exuberant Neuronal Convergence onto Reduced Taste Bud Targets with Preservation of Neural Specificity in Mice Overexpressing Neurotrophin in the Tongue Epithelium

Zaidi

Krimm²,

Whitehead³

2007

J. Neurosci.

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A mouse fungiform taste bud is innervated by only four to five geniculate ganglion neurons; their peripheral fibers do not branch to other buds. We examined whether the degree or specificity of this exclusive innervation pattern is influenced by brain-derived neurotrophic factor (BDNF), a prominent lingual neurotrophin implicated in taste receptoneural development. Labeled ganglion cells were counted after injecting single buds with different color markers in BDNF-lingual-overexpressing (OE) mice. To evaluate the end-organs, taste buds and a class of putative taste receptor cells were counted from progeny of BDNF-OE mice crossbred with green fluorescent protein (GFP) (gustducin) transgenic mice. Fungiform bud numbers in BDNF-OE mice are 35%, yet geniculate neuron numbers are 195%, of wild-type mice. Neurons labeled by single-bud injections in BDNF-OE animals were increased fourfold versus controls. Injecting three buds, each with different color markers, resulted in predominantly single-labeled ganglion cells, a discrete innervation pattern similar to controls. Thus, hyper-innervation of BDNF-OE buds involves many neurons innervating single buds, not increased fiber branching. Therefore, both wild-type and BDNF-OE mice exhibit, in fungiform buds, the same, "discrete" receptoneural pattern, this despite dramatic neurotrophin overexpression-related decreases in bud numbers and increases in innervation density. Hyperinnervation did not affect GFP positive cell numbers; proportions of GFP cells in BDNF-OE buds were the same as in wild-type mice. Total numbers of ganglion cells innervating buds in transgenic mice are similar to controls; the density of taste input to the brain appears maintained despite dramatically reduced receptor organs and increased ganglion cells.

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“…[33][34][35] Trk-A (NGF receptor) protein and mRNA also increase in level and peak at 30 d after ON lesion in tench retina. 36) Ciliary neurotrophic factor (CNTF) also promotes neurite outgrowth of fish RGCs. Although other neurotrophic factors such as NT-6, 37) NT-7, 38) brain derived neurotrophic factor (BDNF) 39) and glial cell line derived neurotrophic factor (GDNF) 40) have been identified in fish, the functional role of these factors in ON regeneration is not yet known.…”

Section: ) Neurotrophic Factors Nerve Growth Factor (Ngf)mentioning

confidence: 95%

Axonal Regeneration of Fish Optic Nerve after Injury

Matsukawa

Arai

Koriyama

et al. 2004

Biological & Pharmaceutical Bulletin

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In the adult mammalian central nervous system (CNS), axonal injury leads to retrograde neuronal degeneration and death in cell soma.1,2) For instance, transection of the rat optic nerve (ON) induces cell death of retinal ganglion cells (RGCs) within 2 weeks. 3,4) In contrast, injury to the fish ON never leads to widely spreading death of RGCs. Goldfish regrow the axons of their RGCs towards the tectum and finally restore their vision, even after ON transection. 5,6) It has been shown that some rat RGCs can regenerate their axons through peripheral nerve grafts.7) This result implies that the inability of CNS neurons to regenerate in mammals is mainly due to a surrounding inhibitory environment, but not to the nature of the neurons themselves. Therefore, two approaches to the reconstruction of injured ONs in mammals have been studied intensively: 1) the use of nerve grafts or transplantation of stem cells [8][9][10] and 2) studying the regeneration mechanism of the mammalian peripheral nervous system (PNS) and fish CNS.11-13) Our goal here is aimed to introduce the works of goldfish ON regeneration after injury and show up the difference between fish and mammals.Since the first description of fish ON regeneration by Sperry's group in the 1950s, there has been a significant increase in the understanding of morphological changes in RGCs and factors which promote neurite outgrowth in optic axons after ON transection. In contrast, there is little knowledge regarding the molecular (genetic) mechanisms and cellular signaling pathways involved in fish ON regeneration after injury. Very recently, we apply a molecular cloning technique to find out ON regenerating molecules in goldfish after nerve injury. We obtain cDNA clones that are upregulated in the fish retina and tectum during ON regeneration. In this article, we review the neurite outgrowth-promoting factors reported by other investigators and our strategies for searching ON regenerating molecules in the goldfish visual system after injury. MORPHOLOGICAL ASPECTS OF FISH ON REGENER-ATIONIn goldfish, unmyelinated sproutings occur at the cut end of the ON within 3 d, and then bundles of 20-30 axonal sproutings penetrate into the cutting edge by 6 d after ON lesion.14) Outgrowth of the leading axons proceeds at 0.3 mm/d after axotomy.15) The regenerating fibers first reach the optic lobe at 10-12 d after ON lesion, and the plexiform layer formed by the regenerating fibers is visible in all areas of the optic tectum at 14-18 d after ON lesion.16) Although the retinotectal connection is formed 20-40 d after ON section, the regenerated retinotectal projection lacks topographic order for up to 40 d. Thereafter, the retinotectal topography slowly improves over several months.17) Thus, the retinotectal connection is initially very rough and then becomes very refined after a long time. This refinement process of the regenerating fibers can be traced using local injections of wheat germ agglutinin conjugated to horse radish peroxidase (WGA-HRP) in the retina. After goldfish ON...

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Neurotrophins and their receptors in the tench retina during optic nerve regeneration

Cited by 55 publications

References 72 publications

Expression of Brain-Derived Neurotrophic Factor and TrkB in the Lateral Line System of Zebrafish During Development

Expression of Brain-Derived Neurotrophic Factor and TrkB in the Lateral Line System of Zebrafish During Development

Exuberant Neuronal Convergence onto Reduced Taste Bud Targets with Preservation of Neural Specificity in Mice Overexpressing Neurotrophin in the Tongue Epithelium

Axonal Regeneration of Fish Optic Nerve after Injury

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