Neurotrophins promote motor neuron survival and are present in embryonic limb bud

Henderson, Chris; Camu, William; Mettling, Clément; Gouin, Annie; Poulsen, Kristian Arild; Karihaloo, Mona; Ruilamas, Janette; Evans, Tony; McMahon, Stephen B.; Armanini, Mark; Berkemeier, Lucy R.; Phillips, Heidi S.; Rosenthal, Arnon

doi:10.1038/363266a0

Cited by 594 publications

(475 citation statements)

References 32 publications

Supporting

Mentioning

446

Contrasting

Unclassified

Order By: Relevance

“…Indeed, after an initial maintenance period with CNTF a switch to BDNF or NT-3 revealed a distinct survival effect of these two members of the NGF gene family (Hughes, unpublished results) . In contrast to chick motoneurons, E-15 rat embryonic motoneurons showed a very marked response to BDNF, NT-3, and NT-4/5 (34,39). Conversely, the survival effect of CNTF in these rat motoneurons was distinctly below the effects observed in chick motoneurons (3,34,39).…”

Section: Discordant Results Originating From Species Differences and mentioning

confidence: 79%

“…The rapidly increasing number of molecules which exhibit neurotrophic actions on motoneurons has also left u s with a se ries of di sco rda nt findings which , a t least for the in vitro observa tions , can be explained by diffe rences in the culture conditions (e. g., serum vs serumfree medium), th e preparation and sel ect ion of m oto neurons, the "time window" se lected for the preparation of motoneurons, and al so hy spec ie s differences (3,10,23,24,33,34,39,68). Mo reove r, there are substa nti a l di screpancies between in vitro and in vivo effects for given molecules.…”

Section: Discordant Results Originating From Species Differences and mentioning

confidence: 99%

“…Thus, LIF, interleukin-6, and oncostatin-M deserve a thorough evaluation as additional potential neurotrophic molecules for motoneurons. Indeed, in vitro and in vivo effects of LIF on motoneurons have been observed (34,39,58). However, the physiological relevance of these observations remains to be established, particularly in view of the fact that a first evaluation of LIF -/-mice did not show any changes in spinal motoneurons either at the morphological or functional levels (84).…”

Section: Thoenen Hughes and Sendtnermentioning

confidence: 99%

“…However, when it became apparent that the mRNAs of BDNF, NT-3, and NT-4 / 5 are expressed in rat skeletal muscle during embryonic development and also adulthood (9,36,55), that the signal-transducing units mediating the effects of BDNF, NT-3, and NT-4/5, namely trkB and trkC, are expressed by motoneurons (26,34), and that the specific retrograde axonal transport of labelled BDNF and NT-3 was demonstrated (18), a reevaluation of the negative results obtained in vitro with chick motoneurons was mandatory. Indeed, independently four laboratories demonstrated an in vivo effect of BDNF on motoneurons (45,78,96,107).…”

Section: Neurotrophic Effects Of Members Of the Ngf Gene Familymentioning

confidence: 99%

“…Progress was predominantly determined by an improvement of the motoneuron cell culture procedures (3,10,12,33,34,39) and the identification of neurotrophic molecules in adult and/or embryonic skeletal muscles with already known neurotrophic actions on other populations of neurons in vitro and in vivo (3,13,34,36,38,39,45,54,58,80). The importance of appropriate culture systems for motoneurons is demonstrated by the fact that, for a long time, no single, defined molecule could be demonstrated to have a convincing trophic action on motoneurons.…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Trophic Support of Motoneurons: Physiological, Pathophysiological, and Therapeutic Implications

Thoenen¹,

Hughes²,

Sendtner³

1993

Experimental Neurology

View full text Add to dashboard Cite

Section: Discordant Results Originating From Species Differences and mentioning

confidence: 79%

Section: Discordant Results Originating From Species Differences and mentioning

confidence: 99%

Section: Thoenen Hughes and Sendtnermentioning

confidence: 99%

Section: Neurotrophic Effects Of Members Of the Ngf Gene Familymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Trophic Support of Motoneurons: Physiological, Pathophysiological, and Therapeutic Implications

Thoenen¹,

Hughes²,

Sendtner³

1993

Experimental Neurology

View full text Add to dashboard Cite

Regional distribution of neurotrophin receptors in the developing auditory brainstem

1996

View full text Add to dashboard Cite

Neuron survival and axonal regeneration become severely limited during early postnatal development. In conjunction with our recent organotypic analysis of regeneration in the auditory midbrain, we wished to determine whether neurotrophins could serve as a trophic substance during the postnatal period. Therefore, the current study examines the development of three neurotrophin receptor tyrosine kinases (TrkA, TrkB, and TrkC) in the gerbil auditory brainstem. Immunoreactivity to TrkA, the nerve growth-factor receptor, was observed in nonneuronal cells during the first two postnatal weeks. In the cochlear nucleus of mature animals, however, there was a TrkA-positive neuronal subpopulation. In contrast, immunoreactivity to TrkB and TrkC (the receptors for brain-derived neurotrophic factor and neurotrophin-3, respectively) displayed a widespread distribution in the auditory brainstem. At postnatal day 0, TrkB and TrkC staining was virtually absent from auditory nuclei, although immunopositive neurons were present in the mesencephalic trigeminal nucleus. By postnatal day 7, TrkB- and TrkC-positive neurons were present in most brainstem auditory nuclei. At postnatal day 15, TrkB immunoreactivity was observed throughout the inferior colliculus (IC), the cochlear nucleus, the medial and lateral nuclei of the trapezoid body, and the lateral superior olive, whereas TrkC labeled only a subpopulation of neurons within the central nucleus of the IC. The TrkB immunoreactivity was present on both neuronal somata and dendrites, whereas TrkC was generally restricted to cell bodies. At postnatal day 30, TrkB immunostaining was observed on most neurons of the IC. The medial and lateral nuclei of the trapezoid body displayed extremely strong TrkB staining, followed by the cochlear nucleus. In contrast, the TrkC immunostaining was decreased dramatically by postnatal day 21. Observations at the ultrastructural level confirmed a neuronal localization of TrkB and TrkC. Immunostaining for both receptors was restricted largely to the postsynaptic density of synaptic profiles in both dendrites and somata. In summary, this study illustrates a differential pattern of immunoreactivity between three neurotrophin receptors during development. The general increase of TrkB expression is well correlated with the onset of sound-evoked activity in this system, and its synaptic localization suggests that it may be involved in the modulation or maintenance of postsynaptic physiology.

show abstract

Gephyrin accumulates at specific plasmalemma loci during neuronal maturation in vitro

1996

View full text Add to dashboard Cite

The distribution of glycine receptor (GlyR)-associated gephyrin has been investigated in rat spinal cord neurons maintained in vitro by means of immunocytochemical techniques. Gephyrin, which is crucial for the stabilization of postsynaptic GlyR microdomains, is present in mature neurons at postsynaptic differentiations. With immunofluorescence, discontinuous patches of gephyrin were detected within the neuronal soma of spinal cord neurons on the 1st day after plating. Subsequently, gephyrin was present at membrane areas that correspond to points of contact between cells or with the culture dish. By the 5th day, gephrin was mostly associated with the MAP2-positive somatodendritic compartment. With immunoelectron microscopy, gephyrin blobs detected at the earliest stages (1-3 days after plating) were found within the cytoplasm or associated with the plasma membrane. Asymmetrically immunostained intercellular contacts were only detected after 5 days, and gephyrin was found in association with clearly differentiated postsynaptic membranes at 7 days. At later stages, we observed gephyrin immunoreactivity only at some synapses. Our results suggest that gephyrin accumulates initially at the locus of cell-to-cell contacts involved in adhesion processes. These localizations may define hot spots for later accumulation of the GlyR and possibly other receptors.

show abstract

Neurotrophins promote motor neuron survival and are present in embryonic limb bud

Cited by 594 publications

References 32 publications

Trophic Support of Motoneurons: Physiological, Pathophysiological, and Therapeutic Implications

Trophic Support of Motoneurons: Physiological, Pathophysiological, and Therapeutic Implications

Regional distribution of neurotrophin receptors in the developing auditory brainstem

Gephyrin accumulates at specific plasmalemma loci during neuronal maturation in vitro

Contact Info

Product

Resources

About