Neutral pH Hydrogen-Enriched Electrolyzed Water Achieves Tumor-Preferential Clonal Growth Inhibition Over Normal Cells and Tumor Invasion Inhibition Concurrently With Intracellular Oxidant Repression

Abstract

The properties and effects of neutral pH hydrogen-enriched electrolyzed water (NHE water) on tumor cells were examined. NHE water diminished hydroxyl radicals as demonstrated by ESR in a cell-free system. Human tongue carcinoma cells HSC-4 were inhibited for either colony formation efficiencies or colony sizes by NHE water without significant inhibition to normal human tongue epithelial-like cells DOK. Furthermore, NHE water caused growth inhibition, cell degeneration, and inhibition of invasion through the reconstituted basement membrane to human fibrosarcoma cells HT-1080. Intracellular oxidants such as hydroperoxides and hydrogen peroxides were scavenged in HSC-4 or HT-1080 cells by NHE water. In the human oral cavity, a dissolved hydrogen concentrations (DH) of NHE water was drastically declined from 1.1 to 0.5 ppm, but settled to 0.3-0.4 ppm until 180 s, upon static holding without gargling. Thus, NHE water was shown to achieve tumor-preferential growth inhibition and tumor invasion together with scavenging of intracellular oxidants, and is expected as a preventive material against tumor progression and invasion.

α-tocopheryl phosphate suppresses tumor invasion concurrently with dynamic morphological changes and delocalization of cortactin from invadopodia

“…Furthermore, ROS in cells act as the second messengers and play important roles in intracellular signaling cascades, which induce and maintain the oncogenic phenotype of cancer cells (29). On the other hand, it is also known that antioxidants can inhibit tumor cell proliferation (13,38,39), invasion and metastasis (14,40,41). Thus, these findings suggest the possibility that scavenging of ROS by antioxidant materials can suppress the tumorigenic actions of ROS and the development of certain cancers.…”

“…Invasiveness of HT-1080 cells into the reconstituted BM Matrigel™ (BD Biosciences, MA, USA) was determined as described previously (13,14). Briefly, cells were detached with phosphate-buffered saline (PBS)-EDTA and gently pipetted to form single-cell suspension in culture medium.…”

The hydrogen molecule as antioxidant therapy: clinical application in hemodialysis and perspectives

“…Studies designed to test the biological activity of electrolyzed water have demonstrated tumor antiproliferative effects [77][78][79] and antidiabetic actions in a diabetic model by amelioration of beta cell oxidative injury [80][81][82]. Drinking enriched water ad libitum ameliorated disuse muscular atrophy after paralysis in rats [58] and protected against cardiac and kidney fibrosis by ischemic/reperfusion of kidney [59] and aging [83] in Dahl SS rats.…”

Effect of electrolyzed water produced using carbon electrodes on HeLa cell proliferation

“…①-W (control) medium was also filtered in the same way as that for ②-W medium ( Figure 3A); this filtration had no effect under the microscope.This process generated 6 types of media (①-W, ①-EW, ②-W, ②-EW, ③-W and ③-EW) that were each sterilized by autoclaving at 121°C for 15 min and then supplemented with 10% FBS, 0.584 g/L glutamine, 100 µg/mL streptomycin, 100 U/mL penicillin, and 10% sodium hydrogen carbonate. 8.05. EW media had a slightly lower pH than that of W media, but all media were still alkaline.…”

“…Recently, several researchers have reported the generation of electrolyzed water of neutral pH by an electrolysis chamber without a diaphragm. Neutral pH hydrogen-enriched electrolyzed water inhibited efficient colony formation of human tongue squamous cell carcinoma-derived HSC-4 cells and growth of human fibrosarcoma HT-1080 cells (8). Slightly acidic electrolyzed water (pH 5.0 to 6.5) produced in an electrolysis chamber without a diaphragm containing a 2% to 6% hydrogen chloride solution exhibited antibacterial effects on oral pathogens and inhibited the proliferation of human gingival fibroblasts (9).…”