Neutralisation of cholera toxin (CT) in jejunal ligated loops (JLL) of rats immunized with a mouse monoclonal (MC) auto-anti-idiotypic (ID) antibody (Ab) against anti-CT ABS

Lucas, G; Cambiaso, Cesar; Vaerman, Jean‐Pierre

doi:10.1007/978-94-009-1848-1_103

Cited by 2 publications

(4 citation statements)

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“…In a similar test, but with a different anti-CT MAb-Lx (5B5-Lx) and a different anti-id MAb (namely, the autoanti-id MAb studied previously [23]), the addition of CT did not influence the agglutination of the anti-CT MAb-Lx by this other auto-anti-id MAb (Fig. 2C).…”

Section: Resultsmentioning

confidence: 63%

“…For some experiments, another auto-anti-id [anti-(anti-CT)] MAb, a monomeric IgM (1) secreted by a clone isolated directly from the spleen of CT-immunized mice (23), was also used.…”

Section: Methodsmentioning

confidence: 99%

“…Several examples of successful anti-id immunizations against various viruses, bacteria, and parasites have already been reported (for reviews, see references 7 and 10), but none has yet been reported against CT. In a preliminary study (23), we reported that rats, after repeated intraperitoneal (i.p.) injections of a mouse auto-anti-id monoclonal antibody (MAb), not only acquired serum and bile anti-CT Ab titers but were also significantly protected against a CT challenge in their ligated gut loops.…”

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confidence: 99%

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Protection of rat intestine against cholera toxin challenge by monoclonal anti-idiotypic antibody immunization via enteral and parenteral routes

1991

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A mouse monoclonal anti-idiotypic (anti-id) immunoglobulin M (IgM) antibody, called MAb2, was raised against a mouse monoclonal anti-cholera toxin (anti-CT) antibody (MAbl). The MAb2 was shown, by competition with CT for MAbl, to bear the internal image of an epitope of CT. MAb2 immunization of rats was performed via the intraperitoneal, intragastric, and intrajejunal routes and compared with immunization of rats with either a control, isotype-and allotype-matched MAb or with CT via the same routes. Both serum IgG and bile IgA anti-CT Ab3's were detected by enzyme-linked immunosorbent assay in anti-id MAb2immunized rats, although their titers were lower than those in CT-immunized rats. No anti-CT antibodies were detected in sera and bile of rats immunized with the control MAb. When tested for degree of gut protection against a CT challenge, rats immunized with MAb2 by the intrajejunal route showed a rather high degree of protection, which was only slightly lower than that of rats immunized with CT via the same route; all rats but one immunized with the control MAb were unprotected. There was, however, no correlation between serum or bile anti-CT titers and degree of gut protection in MAb2-immunized rats. Their serum anti-CT Ab3's were purified by adsorption and elution from a CT immunosorbent and resembled anti-CT MAbl in their unique reactivity with MAb2. This constitutes to our knowledge the second report of protection against a pathogen by anti-id immunization via the enteric route.

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Section: Resultsmentioning

confidence: 63%

“…For some experiments, another auto-anti-id [anti-(anti-CT)] MAb, a monomeric IgM (1) secreted by a clone isolated directly from the spleen of CT-immunized mice (23), was also used.…”

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

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Protection of rat intestine against cholera toxin challenge by monoclonal anti-idiotypic antibody immunization via enteral and parenteral routes

1991

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“…Anti-idiotypic antibodies are also useful to search for a pathogenic role of anti-receptor antibodies in autoimmune diseases [18,19], [2tX) Brussels, Belgium, sitic [22] and bacterial [23,24] infections. We partly succeeded [25,26] in protecting rats against an intestinal CT-challenge by anti-idiotype vaccination. Here, we characterize a new mouse anti-idiotypic MoAb, mAb2, that mimics a receptor-binding epitope of CT and elicits anti-CT antibodies, Ab3, after injections into rabbits.…”

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confidence: 99%

Characterization of an anti-idiotypic MoAb bearing an internal image of the receptor-binding epitope of cholera toxin

Lucas

Cambiaso

Vaerman

1992

Clinical and Experimental Immunology

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A mouse anti-cholera toxin (CT) MoAb, mAb1, specific for the GM1-binding epitope of CT, was used to raise a syngenic anti-idiotypic MoAb, mAb2. Purified mAb2 was specific for mAb1 as shown by latex particle counting immunoassay and ELISA. Several experiments of competition between mAb2 and CT for binding to mAb1 demonstrated that mAb2 bore an internal image of the GM1-binding epitope of CT. Binding of mAb2 to GM1 unambiguously corroborated the mAb1-paratopic specificity of mAb2. Furthermore, mAb2 acted as a CT-surrogate antigen: rabbits injected with mAb2 produced some anti-CT antibodies, Ab3, which resembled mAb1 in specificity as expected. The potential use of this mAb2 as vaccine or as prophylactic agent to prevent CT from binding to its cellular receptor is discussed.

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Neutralisation of cholera toxin (CT) in jejunal ligated loops (JLL) of rats immunized with a mouse monoclonal (MC) auto-anti-idiotypic (ID) antibody (Ab) against anti-CT ABS

Cited by 2 publications

References 5 publications

Protection of rat intestine against cholera toxin challenge by monoclonal anti-idiotypic antibody immunization via enteral and parenteral routes

Protection of rat intestine against cholera toxin challenge by monoclonal anti-idiotypic antibody immunization via enteral and parenteral routes

Characterization of an anti-idiotypic MoAb bearing an internal image of the receptor-binding epitope of cholera toxin

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