2022
DOI: 10.1038/s41467-022-28544-w
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Neutralization against Omicron SARS-CoV-2 from previous non-Omicron infection

Abstract: The spread of the Omicron SARS-CoV-2 variant underscores the importance of analyzing the cross-protection from previous non-Omicron infection. We have developed a high-throughput neutralization assay for Omicron SARS-CoV-2 by engineering the Omicron spike gene into an mNeonGreen USA-WA1/2020 SARS-CoV-2 (isolated in January 2020). Using this assay, we determine the neutralization titers (defined as the maximal serum dilution that inhibited 50% of infectious virus) of patient sera collected at 1- or 6-months aft… Show more

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Cited by 107 publications
(74 citation statements)
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“…The mNG gene was inserted into the open-reading-frame-7 (ORF7) of the viral genome to enable the development of a fluorescent focus reduction neutralization test (FFRNT). 4 Our historical data set indicate that the high-throughput FFRNT produces comparable neutralization results as the gold standard plaque-reduction neutralization test (PRNT; using SARS-CoV-2 without the mNG reporter)…”
Section: Main Textmentioning
confidence: 93%
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“…The mNG gene was inserted into the open-reading-frame-7 (ORF7) of the viral genome to enable the development of a fluorescent focus reduction neutralization test (FFRNT). 4 Our historical data set indicate that the high-throughput FFRNT produces comparable neutralization results as the gold standard plaque-reduction neutralization test (PRNT; using SARS-CoV-2 without the mNG reporter)…”
Section: Main Textmentioning
confidence: 93%
“…The details of FFRNT protocol were reported previously. 4 Briefly, 2.5×10 4 Vero E6 cells per well were seeded in 96-well plates (Greiner Bio-one™). The cells were incubated overnight.…”
Section: Fluorescent Focus Reduction Neutralization Test (Ffrnt)mentioning
confidence: 99%
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“…The standard two‐dose regimen of COVID‐19 vaccination showed limited protective effects against the Omicron variant. 123 The protective effect, neutralizing activity, against the Omicron variant is lost in 50% of individuals within 3 months after two doses of the mRNA‐1273 and BNT162b2 vaccines. 124 However, a significant increase in neutralizing antibody levels was observed after a booster dose.…”
Section: Strategies Against the Omicron Variantmentioning
confidence: 99%
“…The mutation related changes greatly affects the transmissibility of the virus, the symptoms of infection, and host immune response 1719 . In addition to general immune evasion from neutralizing antibodies, Omicron also broadly caused dramatic reduction in the protective efficacy of vaccines and sera 2022 22-24, which leads to a reduction in the options of responding to the SARS-CoV-2 pandemic and forces the generation of more passive prevention policies. Many neutralizing antibodies are disabled due to the large number of and widely scattered mutation sites in the N-terminal domain (NTD) and RBD region of the Omicron variant 23,24 .…”
Section: Discussionmentioning
confidence: 99%