Neutralization of Bacteriophage by the Properdin System

Vunakis, Helen Van; Barlow, James L.; Levine, Lawrence

doi:10.1073/pnas.42.7.391

Cited by 32 publications

(11 citation statements)

References 6 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It was also observed that almost all normal rabbit sera (NRS) contained low background neutralizing activity to several viral antigens, among them PV (2). In the present study the physicochemical nature, cross-reactivity, and avidity of the serum factor responsible for this background neutralization of PV and its relationship to the rapid 19S antibody response, were explored.Normal antibody has been reported to differ from immune antibody in specificity (3) and thermostability (3)(4)(5)(6). While in those studies normal antibody was compared with immune antibody collected at a fairly late stage in the course of immunization, in the present study, normal antibody was compared with the earliest detectable induced antibody.…”

mentioning

confidence: 88%

“…Normal antibody has been reported to differ from immune antibody in specificity (3) and thermostability (3)(4)(5)(6). While in those studies normal antibody was compared with immune antibody collected at a fairly late stage in the course of immunization, in the present study, normal antibody was compared with the earliest detectable induced antibody.…”

mentioning

confidence: 88%

“…The origin and specificity of such background or normal antibodies has long been a matter of controversy. The distinction between normal and immune antibody has been based on reported differences in specificity and thermostability (3)(4)(5)(6).…”

Section: Kinetics and Thermodynamic Parameters Of Neutralization Of Pmentioning

confidence: 99%

See 2 more Smart Citations

The Formation and Properties of Poliovirus-Neutralizing Antibody

Svehag¹

1964

The Journal of Experimental Medicine

View full text Add to dashboard Cite

A previous communication (1) in this series reported that transient, very rapid macroglobulin antibody responses could be induced in rabbits by small doses of poliovirus (PV) antigen. 19S antibody appeared 8 to 16 hours following immunization in such responses. It was also observed that almost all normal rabbit sera (NRS) contained low background neutralizing activity to several viral antigens, among them PV (2). In the present study the physicochemical nature, cross-reactivity, and avidity of the serum factor responsible for this background neutralization of PV and its relationship to the rapid 19S antibody response, were explored.Normal antibody has been reported to differ from immune antibody in specificity (3) and thermostability (3-6). While in those studies normal antibody was compared with immune antibody collected at a fairly late stage in the course of immunization, in the present study, normal antibody was compared with the earliest detectable induced antibody. Under these circumstances it was found that the physicochemical properties, cross-reactivity, and avidity of normal and early immune antibody to PV were similar, if not identical. The thermodynamic data for the neutralization of PV by normal antibody were the same as those of serological reactions employing immune antibody. Materials and MethodsVirus.--Type 1 (Brunhilde), type 2 (MEF-1), and type 3 (Saukett) of poliovirus and Coxsaekie viruses B-4 and B-51 were used. Poliovirus, types I and 2, were cloned on HeLa monolayers. All viruses were grown on HeLa monolayers, and virus stocks were partly purified and concentrated by differential centrifugation prior to their storage at -20°C. The virus stocks contained about 1 Dg protein per 106.~ plaque-forming units (PFU).

show abstract

mentioning

confidence: 88%

mentioning

confidence: 88%

See 1 more Smart Citation

The Formation and Properties of Poliovirus-Neutralizing Antibody

Svehag¹

1964

The Journal of Experimental Medicine

View full text Add to dashboard Cite

show abstract

“…(Later, several other factors were recognized.) Numerous reports indicated that the 'properdin system' kills certain bacteria, neutralizes some viruses and, possibly, plays a role in cancer immu nity [44][45][46][47][48][49].…”

Section: Discovery Of the Alternative Activation Pathwaymentioning

confidence: 99%

Complement

Mayer¹

1984

Complement

View full text Add to dashboard Cite

“…Curiously it has been discovered that phage "antibodies" are present in germfree guinea pigs.57 Similar "antibodies" have been found in normd human ~erum. [58][59] At first it might be suspected that such "antibodies" are formed in response to virus leakage through the intestinal wall. However, the finding of such "antibodies" in germ-free animals, which contain no bacteria to form phage in their gastrointestinal (GI) tract, makes it unlikely that these "antibodies" arose in response to phage in the GI tract.…”

Section: Antiphage Antibodiesmentioning

confidence: 99%

Bacteriophage Interactions With Higher Organisms

Merril

1974

Trans NY Acad Sci

View full text Add to dashboard Cite

Bacteriophages were discovered 59 years ago. Despite the widespread distribution of these viruses, the extent of their interaction with higher organisms, including man, is still largely unknown. Recently, it has been shown that some phages carry genes that code for toxins, as in the case of phage β, which codes for diphtheria toxin. There have also been reports of phage genome transcription and translation in in vitro eukaryotic systems. The word bacteriophage conveys the historical nature of the discovery of this class of viruses, it does not define the limits of the viruses effects or host range. The recent discoveries of bacteriophage in commercial fetal calf serum and human vaccines should stimulate efforts to learn of phage interactions with eukaryotic systems.

show abstract

Neutralization of Bacteriophage by the Properdin System

Cited by 32 publications

References 6 publications

The Formation and Properties of Poliovirus-Neutralizing Antibody

The Formation and Properties of Poliovirus-Neutralizing Antibody

Complement

Bacteriophage Interactions With Higher Organisms

Contact Info

Product

Resources

About