Neutralization of Clostridium difficile Toxin A with Single-domain Antibodies Targeting the Cell Receptor Binding Domain

Hussack, Greg; Arbabi‐Ghahroudi, Mehdi; Faassen, Henk van; Songer, J. Glenn; Ng, Kenneth; MacKenzie, Ross; Tanha, Jamshid

doi:10.1074/jbc.m110.198754

Cited by 131 publications

(152 citation statements)

References 98 publications

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“…Indeed, after immunization of Camelidae species, milk instead of blood serum might be a dietary source of single-domain antibody fragments (V H Hs) able to bind therapeutic targets. For example, llama's V H Hs can specifically target the cell receptor domains of toxins of Clostridium difficile (Hussack et al, 2010). In addition, the V H Hs of small size (ca.…”

Section: Resultsmentioning

confidence: 99%

Fast protein liquid chromatography of camel ?-lactalbumin fraction with radical scavenging activity

Halima

Jrad

Khorchani

et al. 2014

Emir. J. Food Agric

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The aim of this study was to investigate the radical-scavenging properties towards a stable radical cation, ABTS, of Camelus dromedarius whey proteins (CWP) separated onto a cation-exchanger by fast protein liquid chromatography. The highest activities were found for CWP and fraction F1 mainly composed of α-lactalbumin. Fractions F2, F3 and F4 contained a mixture of lactoferrin, immunoglobulins G and probably camel whey basic protein (CWBP). These three fractions displayed low radical-scavenging activities. Lactoferrin was eluted almost pure in the last fraction (F5) but did not possess detectable radical-scavenging activity. The present results suggested that the cation-exchange chromatography is of great interest to yield, in a single step, whey protein fractions with various biological activities, i.e. a highly-enriched α-lactalbumin fraction displaying efficient antioxidant activity, a fraction (pool of F2-F4) mainly composed of heavy-chain immunoglobulins potentially interesting for human therapy and a fraction of pure lactoferrin having numerous biological activities such as antimicrobial and immunomodulating properties.

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Section: Resultsmentioning

confidence: 99%

Fast protein liquid chromatography of camel ?-lactalbumin fraction with radical scavenging activity

Halima

Jrad

Khorchani

et al. 2014

Emir. J. Food Agric

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“…By far, nanobodies for antiscorpion toxins, antibacterial toxins, and anti-snake venom are actively being investigated. [83][84][85][86][87] Owing to their small size and extended CDR3, nanobodies also showed special advantages as therapeutics for infectious disease, including the infection of viruses, bacteria, and parasites, over conventional antibodies that usually obstruct the access of hidden and essential epitopes on pathogens. [88][89][90][91][92][93][94][95][96] The added value of the nanobodies as targeting therapeutics stems from their capacity to distinguish the cognate target from closely related variants.…”

Section: Nanobodies As Targeting Therapeuticsmentioning

confidence: 99%

Nanobody-derived nanobiotechnology tool kits for diverse biomedical and biotechnology applications

Wang

Fan

Shao

et al. 2016

IJN

125

107

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Owing to peculiar properties of nanobody, including nanoscale size, robust structure, stable and soluble behaviors in aqueous solution, reversible refolding, high affinity and specificity for only one cognate target, superior cryptic cleft accessibility, and deep tissue penetration, as well as a sustainable source, it has been an ideal research tool for the development of sophisticated nanobiotechnologies. Currently, the nanobody has been evolved into versatile research and application tool kits for diverse biomedical and biotechnology applications. Various nanobody-derived formats, including the nanobody itself, the radionuclide or fluorescent-labeled nanobodies, nanobody homo- or heteromultimers, nanobody-coated nanoparticles, and nanobody-displayed bacteriophages, have been successfully demonstrated as powerful nanobiotechnological tool kits for basic biomedical research, targeting drug delivery and therapy, disease diagnosis, bioimaging, and agricultural and plant protection. These applications indicate a special advantage of these nanobody-derived technologies, already surpassing the “me-too” products of other equivalent binders, such as the full-length antibodies, single-chain variable fragments, antigen-binding fragments, targeting peptides, and DNA-based aptamers. In this review, we summarize the current state of the art in nanobody research, focusing on the nanobody structural features, nanobody production approach, nanobody-derived nanobiotechnology tool kits, and the potentially diverse applications in biomedicine and biotechnology. The future trends, challenges, and limitations of the nanobody-derived nanobiotechnology tool kits are also discussed.

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“…In addition, several Nbs targeting bacterial toxins and snake venoms have been reported. 42,77 A range of other human pathogens, such as Vibrio cholerae and Salmonella enterica, might also be suitable for Nb targeting.…”

Section: Siontoroumentioning

confidence: 99%

Nanobodies as novel agents for disease diagnosis and therapy

Siontorou¹

2013

IJN

126

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Neutralization of Clostridium difficile Toxin A with Single-domain Antibodies Targeting the Cell Receptor Binding Domain

Cited by 131 publications

References 98 publications

Fast protein liquid chromatography of camel ?-lactalbumin fraction with radical scavenging activity

Fast protein liquid chromatography of camel ?-lactalbumin fraction with radical scavenging activity

Nanobody-derived nanobiotechnology tool kits for diverse biomedical and biotechnology applications

Nanobodies as novel agents for disease diagnosis and therapy

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