Neutralizing monoclonal antibodies directed to infectious bovine rhinotracheitis virus

Abstract

Infectious bovine rhinotracheitis virus (IBRV) has been shown in this report to have thirty-three polypeptides. Ten of the eleven polypeptides which can be labeled with (3H)-glucosamine are located on the surface of the virus since they can be surface labeled with sodium boro(3H)hydride. In order to define the immunologically important viral proteins, monoclonal antibodies were prepared against the virus and selected for their ability to neutralize infectivity. Four such hybridoma lines were obtained for characterization of the antigens that elicit neutralizing antibodies. The viral polypeptides were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and the specificity of each monoclonal antibody was determined by "Western" blot analysis and/or by immunoprecipitation of (35S)-methionine and (3H)-glucosamine labeled infected cell lysates by the monoclonal antibodies. One monoclonal antibody reacted with two glycoproteins, gp135 and gp78a, on the "Western" blot but immunoprecipitated three glycoproteins, gp135, gp78a, and gp54 from labeled infected cell lysates. The other three monoclonal antibodies immunoprecipitated a single glycoprotein, gp78b, from (3H)-glucosamine labeled infected cell lysates but not from (35S)-methionine labeled infected cell lysates.

Bovine herpesvirus type 1 gp87 mediates both attachment of virions to susceptible cells and hemagglutination

“…Like some other herpesviruses, BHV-1 can cause a long-term, latent infection in sensory ganglia and can be reactivated after stress or corticosteroid treatment [1,13,30,34,37]. BHV-1 specifies at least three glycopr0teins with molecular weights of 117-130kd (a disulfide-linked complex composed of two different sized subunits), 87-97kd (which possibly forms a dimer), and 71-105kd, each of which induces the corresponding neutralizing antibodies [4,6,25,31,42]. These glycoproteins have also been reported to be involved in an-tibody and complement-mediated cytolysis [27, 421 and antibody-dependent cell-mediated cytotoxicity [41 ].…”

Applications of Monoclonal Antibodies in Animal Health and Production

“…The authors further demonstrated that a combination of MAbs had a synergistic effect on virus neutralization, showing that more than one epitope was involved in this biological property. Chang et al (1986) also used MAbs to demonstrate which BHV-1 glycoproteins are involved in virus neutralization; but unfortunately differences in nomenclature assigned by the two groups prevent direct comparison of their data.…”

Gene mapping of infectious bovine rhinotracheitis viral DNA genome

“…BHV-I specifies more than 25 structural polypeptides of which probably 11 are glycosylated [2,16]. The major glycoproteins are gI, a 130kDa protein constituted of 74 and 55kDa subunits, gII, a 108 kDa polypeptide, gIII, a 90 kDa protein, and gIV, a 71 kDa protein [3,5,26]. Glycoproteins, being located on the viral envelope and on the surface of virus-infected cells [8,17,28], play important roles during viral infection such as recognizing receptor sites for attachment and penetration of virus in cells.…”