The influence of the mass difference between the charged and neutral pions on the low-energy characteristics of nucleon-nucleon interaction in the 1 S 0 spin-singlet state is studied within the framework of the effective-range approximation. By making use of the experimental singlet neutron-proton scattering parameters and the experimental value of neutron-neutron virtualstate energy, the following values were obtained for the neutron-neutron scattering length and effective range: a nn = −16.59(117) fm, r nn = 2.83(11) fm. The calculated neutron-neutron scattering length a nn is in good agreement with one of the two well known and differing experimental values of this quantity, and the calculated effective range r nn is also in good agreement with present-day experimental results.