Neutron Spin Echo Spectroscopy as a Unique Probe for Lipid Membrane Dynamics and Membrane-Protein Interactions

Kumarage, Teshani; Nguyen, Julie; Ashkar, Rana

doi:10.3791/62396

Cited by 5 publications

(3 citation statements)

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“…Another classical method for elucidating the structural and dynamic changes that happens during lipid protein interaction include neutron scattering techniques such as inelastic and quasielastic neutron scattering (Swenson et al, 2008;Wood and Zaccai, 2008;Armstrong et al, 2012) as well as neutron spin echo (Kumarage et al, 2021). The quasielastic neutron scattering data can be converted to the dynamic nature of lipid membranes based on scattering laws (Qian et al, 2020).…”

Section: Neutron Scattering Studiesmentioning

confidence: 99%

Pore Forming Protein Induced Biomembrane Reorganization and Dynamics: A Focused Review

Ponmalar

Sarangi

Basu

et al. 2021

Front. Mol. Biosci.

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Pore forming proteins are a broad class of pathogenic proteins secreted by organisms as virulence factors due to their ability to form pores on the target cell membrane. Bacterial pore forming toxins (PFTs) belong to a subclass of pore forming proteins widely implicated in bacterial infections. Although the action of PFTs on target cells have been widely investigated, the underlying membrane response of lipids during membrane binding and pore formation has received less attention. With the advent of superresolution microscopy as well as the ability to carry out molecular dynamics (MD) simulations of the large protein membrane assemblies, novel microscopic insights on the pore forming mechanism have emerged over the last decade. In this review, we focus primarily on results collated in our laboratory which probe dynamic lipid reorganization induced in the plasma membrane during various stages of pore formation by two archetypal bacterial PFTs, cytolysin A (ClyA), an α-toxin and listeriolysin O (LLO), a β-toxin. The extent of lipid perturbation is dependent on both the secondary structure of the membrane inserted motifs of pore complex as well as the topological variations of the pore complex. Using confocal and superresolution stimulated emission depletion (STED) fluorescence correlation spectroscopy (FCS) and MD simulations, lipid diffusion, cholesterol reorganization and deviations from Brownian diffusion are correlated with the oligomeric state of the membrane bound protein as well as the underlying membrane composition. Deviations from free diffusion are typically observed at length scales below ∼130 nm to reveal the presence of local dynamical heterogeneities that emerge at the nanoscale—driven in part by preferential protein binding to cholesterol and domains present in the lipid membrane. Interrogating the lipid dynamics at the nanoscale allows us further differentiate between binding and pore formation of β- and α-PFTs to specific domains in the membrane. The molecular insights gained from the intricate coupling that occurs between proteins and membrane lipids and receptors during pore formation are expected to improve our understanding of the virulent action of PFTs.

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Section: Neutron Scattering Studiesmentioning

confidence: 99%

Pore Forming Protein Induced Biomembrane Reorganization and Dynamics: A Focused Review

Ponmalar

Sarangi

Basu

et al. 2021

Front. Mol. Biosci.

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“…Efforts are currently underway to see if this effect can be measured using known geometric inhibitors (e.g., lysolipids), [27] by using layers of spherical vesicles (e.g., 120 nm SUVs, Figure S19, Supporting Information), [38] or through neutron spin echo spectroscopy. [39] Figure 8. Graphical summary of the interaction and impact of amphipathic copolymers with lipid interfaces.…”

Section: Interaction Of Copolymers With Lipid Bilayersmentioning

confidence: 99%

Measuring the Interactions and Influence of Amphipathic Copolymers with Lipid Monolayers and Bilayers as Models of Biological Membranes

Frost,

Wiedbrauk,

Graham

et al. 2023

Macro Chemistry & Physics

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Amphipathic copolymers are useful materials for nanomedicine, owing to their ability to self‐assemble into nanoparticles, act as surfactants for inorganic materials, or for their favorable interactions with lipid membranes. Despite their widespread use, there is still a range of questions about the physicochemical properties that are necessary to drive their interactions at biological interfaces. To fully understand these interactions requires a diverse range of complementary analytical techniques. In this work, a library of neutral amphipathic methacrylate copolymers is synthesized by reversible addition‐fragmentation chain‐transfer polymerization (RAFT) polymerization, to investigate the effect of polymer composition and nature of the hydrophobic comonomer on interactions with model lipid membranes. These materials are shown to interact with Langmuir lipid monolayers, and neutron reflectometry demonstrates that hydrophobic interactions lead to the polymers intercalating with the monolayers. More complex models of lipid bilayers are studied using an in situ quartz crystal microbalance (QCM) model and shows while the composition and hydrophobic comonomer affect the stability of these interactions, there is no effect on the viscoelasticity of the lipid membranes. The in‐depth understanding of these interfacial interactions afforded by this suite of analytical tools will allow for more complex copolymers to be studied, providing a greater understanding of key processes in nanomedicine, such as cellular entry and endosomal escape.

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“…This is a consequence of the wavelength dependence of both Q and the accessible Fourier times. 117 Therefore, measurements at higher Q (i.e. shorter length scales) have a lower upper limit of accessible timescales.…”

Section: Neutron Spectroscopy: a Unique Tool For Probing Molecular An...mentioning

confidence: 99%

The dynamic face of lipid membranes

Gupta

Ashkar

2021

Soft Matter

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Neutron Spin Echo Spectroscopy as a Unique Probe for Lipid Membrane Dynamics and Membrane-Protein Interactions

Cited by 5 publications

References 0 publications

Pore Forming Protein Induced Biomembrane Reorganization and Dynamics: A Focused Review

Pore Forming Protein Induced Biomembrane Reorganization and Dynamics: A Focused Review

Measuring the Interactions and Influence of Amphipathic Copolymers with Lipid Monolayers and Bilayers as Models of Biological Membranes

The dynamic face of lipid membranes

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