Neutrophil Activation by Helicobacter pylori Lipopolysaccharides

Nielsen, Henrik; Birkholz, S.; Andersen, Lis Smedegaard; Moran, Anthony P.

doi:10.1093/infdis/170.1.135

Cited by 103 publications

(64 citation statements)

References 36 publications

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“…Gram-negative rods contain lipopolysaccharide (LPS). LPS is a well-known virulence factor which promotes infection by interference with the host immune response (12) and can activate neutrophils (12,23,27). Although the reason why growth of the microflora in the small intestine was changed by the BFMeT treatment is still unknown.…”

Section: Discussionmentioning

confidence: 99%

Protective Effects of a Culture Supernatant of Lactobacillus acidophilus and Antioxidants on Ileal Ulcer Formation in Rats Treated with a Nonsteroidal Antiinflammatory Drug

Bing

Kinouchi

Kataoka

et al. 1998

Microbiology and Immunology

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Ileal ulcers and thiobarbituric acid (TBA)‐reactive substances in the ileal mucosa were induced in rats treated with a nonsteroidal antiinflammatory drug, 5‐bromo‐2‐(4‐fluorophenyl)‐3‐(4‐methylsulfonylphenyl)thiophene (BFMeT), at a dose of 1,000 mg/kg administered with tap water as drinking water. However, the formation of ileal ulcers and TBA‐reactive substances in the ileal mucosa was repressed by giving the animals a culture supernatant of Lactobacillus acidophilus as drinking water. We measured the antioxidative activity of the culture supernatant and found that the supernatant inhibited the formation of t‐butyl hydroperoxide‐induced TBA‐reactive substances in erythrocyte membrane ghosts. Therefore, the effects of various known antioxidative compounds on the ileal ulcer formation induced by BFMeT were investigated. While α‐tocopherol, t‐butyl‐1,4‐hydroxyanisole and allopurinol did not repress ulcer formation after BFMeT treatment, ascorbic acid, dimethyl sulfoxide, glutathione and β‐carotene significantly inhibited formation. Among these compounds, ascorbic acid was the most effective. Accumulation of TBA‐reactive substances in the ileal mucosa after BFMeT treatment also decreased significantly in rats treated with ascorbic acid. In addition, the percentage of Gram‐negative rods in the ileal contents of rats treated with BFMeT and tap water was dramatically increased, but it was not increased in rats treated with BFMeT and these antioxidants. A positive correlation between the percentage of Gram‐negative rods and the number of ileal ulcers was also observed. These results suggest that lipid peroxidation mediated by oxygen radicals plays an important role in the induction of ileal ulcers by BFMeT in rats, and that lipopolysaccharide‐activated neutrophils probably produce highly reactive hypochlorous acid and hydrogen peroxide, which are inactivated by ascorbic acid and glutathione, respectively.

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Section: Discussionmentioning

confidence: 99%

Protective Effects of a Culture Supernatant of Lactobacillus acidophilus and Antioxidants on Ileal Ulcer Formation in Rats Treated with a Nonsteroidal Antiinflammatory Drug

Bing

Kinouchi

Kataoka

et al. 1998

Microbiology and Immunology

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“…Expression profiling analysis of the B. contaminans bceF::Tp mutant indicated an upregulation of the lpxO gene, which encodes a putative dioxygenase required for acyl chain hydroxylation (7), suggesting that different degrees of 2-hydroxylation might be present with bceF::Tp mutant and wild-type strains. Modified lipid A structures with different phosphorylation or acylation patterns elicit different host immune responses (65,66). Nevertheless, Palfreyman and coauthors showed that B. cepacia complex cell-free supernatants can also increase IL-8 production, suggesting that other factors besides LPS can enhance the immune response (67).…”

Section: Discussionmentioning

confidence: 99%

The Tyrosine Kinase BceF and the Phosphotyrosine Phosphatase BceD of Burkholderia contaminans Are Required for Efficient Invasion and Epithelial Disruption of a Cystic Fibrosis Lung Epithelial Cell Line

et al. 2015

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Bacterial tyrosine kinases and their cognate protein tyrosine phosphatases are best known for regulating the biosynthesis of polysaccharides. Moreover, their roles in the stress response, DNA metabolism, cell division, and virulence have also been documented. The aim of this study was to investigate the pathogenicity and potential mechanisms of virulence dependent on the tyrosine kinase BceF and phosphotyrosine phosphatase BceD of the cystic fibrosis opportunistic pathogen Burkholderia contaminans IST408. The insertion mutants bceD::Tp and bceF::Tp showed similar attenuation of adhesion and invasion of the cystic fibrosis lung epithelial cell line CFBE41o-compared to the parental strain B. contaminans IST408. In the absence of bceD or bceF genes, B. contaminans also showed a reduction in the ability to translocate across polarized epithelial cell monolayers, demonstrated by a higher transepithelial electrical resistance, reduced flux of fluorescein isothiocyanate-labeled bovine serum albumin, and higher levels of tight junction proteins ZO-1, occludin, and claudin-1 present in monolayers exposed to these bacterial mutants. Furthermore, bceD::Tp and bceF::Tp mutants induced lower levels of interleukin-6 (IL-6) and IL-8 release than the parental strain. In conclusion, although the mechanisms of pathogenicity dependent on BceD and BceF are not understood, these proteins contribute to the virulence of Burkholderia by enhancement of cell attachment and invasion, disruption of epithelial integrity, and modulation of the proinflammatory response.

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“…This indicates that ROS was probably induced (in at least some neutrophil populations) by LPS or cytokines in the circulation as a result of preinoculation with 1326/28⌽ r . Stimulation of human neutrophil ROS has previously been shown to occur in response to LPS (27,43), particularly rough forms (33), and the stimulatory effect of gamma interferon on neutrophil gp91-induced ROS has been widely studied with respect to chronic granulomatous disease (12,32,34). TNF-␣ production in response to Staphylococcus aureus also stimulates neutrophil ROS, although the ROS pathway in the latter case appears to involve myeloperoxidase rather than NADPH phox (24).…”

Section: Discussionmentioning

confidence: 99%

Stimulation of gp91 Phagocytic Oxidase and Reactive Oxygen Species in Neutrophils by an AvirulentSalmonella entericaSerovar Infantis Strain Protects Gnotobiotic Piglets from Lethal Challenge with Serovar Typhimurium Strain F98 without Inducing Intestinal Pathology

Foster¹,

Hulme²,

Lovell³

et al. 2005

Infect Immun

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Preinoculation of susceptible 5-day-old gnotobiotic piglets with Salmonella enterica serovar Infantis strain 1326/28⌽ r stimulates neutrophil migration into the intestine, which rapidly protects the pigs against a subsequent (normally lethal) challenge with S. enterica serovar Typhimurium strain F98. Here we show that inoculation with either 1326/28⌽ r or F98 activated reactive oxygen species (ROS) in neutrophils via NADPH pathways in vivo and in vitro and that the survival of both Salmonella strains was increased if neutrophils were cocultured with the ROS inhibitor N-acetylcysteine (captopril). Neither F98 nor 1326/28⌽ r significantly increased reactive nitrogen species (RNS) levels in neutrophils isolated from uninfected pigs. Our results indicate the following: (i) rapid protection of highly susceptible gnotobiotic piglets against F98-induced gastroenteritis by preinoculation with 1326/28⌽ r is likely to be due to stimulation of ROS-producing neutrophils in the intestinal epithelium prior to challenge with the lethal strain; (ii) pathological lesions of the intestine during severe gastroenteritis are not necessarily induced by neutrophil migration per se; and (iii) if neutrophil migration into the intestine is responsible for pathology, then neither increased production of ROS or RNS (in pigs inoculated with the lethal strain) nor reduced production (in protected pigs in which pathological lesions are ameliorated by preinoculation with 1326/28⌽ r ) can account for this phenomenon.

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Neutrophil Activation by Helicobacter pylori Lipopolysaccharides

Cited by 103 publications

References 36 publications

Protective Effects of a Culture Supernatant of Lactobacillus acidophilus and Antioxidants on Ileal Ulcer Formation in Rats Treated with a Nonsteroidal Antiinflammatory Drug

Protective Effects of a Culture Supernatant of Lactobacillus acidophilus and Antioxidants on Ileal Ulcer Formation in Rats Treated with a Nonsteroidal Antiinflammatory Drug

The Tyrosine Kinase BceF and the Phosphotyrosine Phosphatase BceD of Burkholderia contaminans Are Required for Efficient Invasion and Epithelial Disruption of a Cystic Fibrosis Lung Epithelial Cell Line

Stimulation of gp91 Phagocytic Oxidase and Reactive Oxygen Species in Neutrophils by an AvirulentSalmonella entericaSerovar Infantis Strain Protects Gnotobiotic Piglets from Lethal Challenge with Serovar Typhimurium Strain F98 without Inducing Intestinal Pathology

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